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Articles by Bytul M. Rahman
Total Records ( 3 ) for Bytul M. Rahman
  Bytul M. Rahman , M.S.A. Bhuiyan , M. Golam Sadik and A.S.M. Anisuzzman
  The sub-acute toxicity study of 3,6-dimethyl-4-ethyl-0-acetyl benzene (BM-5), was carried out on Long Evan’s rats using daily administration (300 μg/rat/day) of compound for 14 consecutive days. Non-significant differences between weight of compound receiving rats and control rats (48±0.816 vs 46±0.816) were found. The change in hematological parameters was found to be nonsignificant (total count of RBC, 5.1±0.081 vs 4.63±0.094; white blood cell, 7.33±0.124 vs 5.73±0.124; platelets, 361666±10274 vs 261666±8498, (cell/ml) x106; percentage of hemoglobin 65.67±1.60 vs 56±0.816; ESR, 25±0.816 vs 19.67±0.471 for experimental and control rats, respectively) and biochemical parameters (serum glutamate pyruvate transaminase, 8.5±0.408 vs 8.33 ±0.471 IU L-1; serum glutamate oxaloacetate transaminase, 9.83±0.235 vs 9.33±0.234 IU L-1; bilirubin, 0.36±0.104 vs 0.34±0.016 μg dL-1; creatinine, 0.67 ±0.089 vs 0.61±0.009 mg dL-1; urea, 18.83±0.235 vs 18.5±0.408 mg dL-1; for experimental and control rats). Therefore, the changes in body weight, hematological and biochemical parameters were statistically non-significant. No detectable abnormalities were found in histopathology of heart, kidney, liver and lung in experimental group of rats as compared with that of the control group of rats.
  Bytul M. Rahman , M.S.A. Bhuiyan , M.A.A. Al-Bari and A.S.M. Anisuzzaman
  This investigation was an attempt to determine the primary selection of the compounds as therapeutic agents, isolated from an antagonistic Streptomyces species. The antimicrobial metabolites were extracted by CHCl3 from the fermentation broth of organism. The antimicrobial activity profile of the crude metabolites (M) as well as two isolated compounds (BM-3 and BM-5) from the crude metabolites, was interesting against some pathogenic bacteria. The minimum inhibitory concentration (MIC) of the crude metabolite (M) and compounds against six pathogenic bacteria was found to be between 16 and 64 Fg ml-1 respectively.
  Masuder Rahman , Bytul M. Rahman and Bahanur Rahman
  A total of 17 Escherichia coli isolated from 24 fresh faecal samples of broiler and layer were screened for their antibiograms and plasmid profiles. The overall recovery rate of E. coli from faecal samples was 70.83%. All E. coli strains were analyzed to determine their susceptibility patterns to 8 commonly used antibiotics (ampicillin, cephradine, chloramphenicol, ciprofloxacin, gentamicin, streptomycin, tetracycline and sulphamethoxazole) belonging to different groups. From the antibiogram study it was revealed that 87.50% E. coli isolated from broiler were resistant to both ampicillin and sulphamethoxazole. Only 37.50% broiler isolates were highly sensitive to gentamicin and 50% isolates to chloramphenicol. All the E. coli isolates of layer were completely resistant (100%) to sulphamethoxazole and about fifty 5% of the isolates (55.55%) were resistant to both streptomycin and tetracycline. E. coli isolated from layer were found to be highly sensitive (44.44%) to chloramphenicol and 66.66% were also highly sensitive to gentamicin. Plasmid profile of 17 isolates was analyzed by 0.8% agarose gel electrophoresis. A total of 8 different plasmid bands of different size were estimated by eye comparing to reference marker. The estimated size of the bands were 3.25, 5.20, 6.00, 8.00, 15.0, 30.0, 33.5 and 38.0 kbp. Plasmid profile analysis of the isolated E. coli revealed that the isolates carrying multiple plasmids which might be the cause of various degrees of antibiotic resistant. The plasmids were distributed at random in the isolated E. coli strains and there was no remarkable interrelationship between antibiotic resistance and plasmid present. In most of the cases, strains having similar plasmid bands but confer resistant to different antibiotics. In some cases, isolates showed resistance to antibiotics but did not harbor any plasmid indicating that chromosomal DNA may carry the genes that confer resistance to antibiotics.
 
 
 
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