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Articles by Brajesh Kumar
Total Records ( 3 ) for Brajesh Kumar
  Brajesh Kumar , Gopal Singh , Vinit Pratap Singh , Jaydeep Patil , Prashant Mishra , Debjani Choudhury and Seweta Srivastava
  Background and Objective: Due to the ability of Pleurotus species to convert crop residues into food protein, oyster mushrooms are least expensive commercial mushrooms and also easy to grow at various temperature ranges from 20-26°C with 75-85% relative humidity. With respect to these facts the present study was undertaken to assess the influence of different inorganic additives (0.075%) viz., salicylic acid, potassium di-hydrogen orthophosphate, magnesium sulphate, zinc sulphate, potassium chloride, ferrous sulphate and copper sulphate on the yield of two Pleurotus species i.e., P. florida and P. flabellatus. Materials and Methods: The experiment was carried out by using seven inorganic additives in wheat straw for cultivation of Pleurotus species (P. florida and P. flabellatus). Observations were recorded and biological efficiency was calculated for cropping period, days for spawn run, days of pin head initiation, number of loab, number of fruiting body, average weight of fruiting body (g/FB) and yield (g kg–1 dry substrate). Results: Data revealed that 0.075% concentration of different inorganic additives was significantly increased the yield compared to control. Maximum yield (g kg–1 dry substrate) was observed in magnesium sulphate 695.00 g followed by potassium di-hydrogen orthophosphate 660.00 g while minimum yield was found in zinc sulphate 505.00 g of P. florida. In case of P. flabellatus maximum yield observed in potassium di-hydrogen orthophosphate was 665.00 g followed by magnesium sulphate 625.00 g while minimum yield was found in zinc sulphate 517.00 g. Conclusion: It was concluded from the above findings that the magnesium sulphate and potassium di-hydrogen orthophosphate were proved as potential inorganic additives followed by zinc sulphate causing significant increase in spawn run, cropping period and yield of the two test species of oyster mushroom viz., P. florida and P. flabellatus. These chemical additives were also very cost effective and having no residual effect on the quality and taste of mushroom.
  Vipul Kumar , Vinit Pratap Singh , Brajesh Kumar , Seweta Srivastava and Bipen Kumar
  Background and Objective: Sugarcane (Saccharum spp.) is a commercially important cash crop of India utilized for the production of sucrose, ethanol, biofuel and fiber-related commodities. Amongst the foliar diseases, the pokkah boeng disease is becoming the major disease of sugarcane. In view of importance of this disease in sugarcane cultivation in India, a plan has been outlined for its study on different parameters of soil properties and their management by using some chemotherapeutants. Materials and Methods: Study on the different inoculation techniques on suitable host variety of sugarcane was done by inoculating with Fusarium moniliforme under suitable temperature (25-30°C), humidity (80-85%) and rainfall (300-400 mm.) under natural conditions. Sugarcane is grown on varieties of soil from sandy loam to heavy clay but is grown best on well drained sandy loam soil. Results: Data revealed that sandy clay loam soil and ph 6.5-7.5 exhibited highest incidence of disease among all type of soils. It was also observed that the percentage pokkah boeng incidence increased with the increase in moisture content of soil. In the soil temperature study, results revealed that 24-29°C temperature was favourable for disease development but maximum disease incidence was recorded at 27.5°C. Copper oxychloride was found more effective against pathogen out of all the chemical fungicides used during the experiment. Conclusion: Sandy clay loam exhibited highest incidence of disease and copper oxychloride was found more effective fungicide against Fusarium moniliforme.
  Thomas G. Gillette , Brajesh Kumar , David Thompson , Clive A. Slaughter and George N. DeMartino
  The 26 S proteasome is an energy-dependent protease that degrades proteins modified with polyubiquitin chains. It is assembled from two multi-protein subcomplexes: a protease (20 S proteasome) and an ATPase regulatory complex (PA700 or 19 S regulatory particle) that contains six different AAA family subunits (Rpt1 to -6). Here we show that binding of PA700 to the 20 S proteasome is mediated by the COOH termini of two (Rpt2 and Rpt5) of the six Rpt subunits that constitute the interaction surface between the subcomplexes. COOH-terminal peptides of either Rpt2 or Rpt5 bind to the 20 S proteasome and activate hydrolysis of short peptide substrates. Simultaneous binding of both COOH-terminal peptides had additive effects on peptide substrate hydrolysis, suggesting that they bind to distinct sites on the proteasome. In contrast, only the Rpt5 peptide activated hydrolysis of protein substrates. Nevertheless, the COOH-terminal peptide of Rpt2 greatly enhanced this effect, suggesting that proteasome activation is a multistate process. Rpt2 and Rpt5 COOH-terminal peptides cross-linked to different but specific subunits of the 20 S proteasome. These results reveal critical roles of COOH termini of Rpt subunits of PA700 in the assembly and activation of eukaryotic 26 S proteasome. Moreover, they support a model in which Rpt subunits bind to dedicated sites on the proteasome and play specific, nonequivalent roles in the asymmetric assembly and activation of the 26 S proteasome.
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