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Articles by Boubaker Hassan
Total Records ( 4 ) for Boubaker Hassan
  Cherifi Khalil , Boufous El Houssein , Boubaker Hassan and Msanda Fouad
  Objective: The purpose of this study was to assess and compare the seed germination response of six Acacia species under different NaCl concentrations in order to explore opportunities for selection and breeding salt tolerant genotypes. Methodology: Germination of seeds was evaluated under salt stresses using 5 treatment levels: 0, 100, 200, 300 and 400 mM of NaCl. Corrected germination rate (GC), germination rate index (GRI) and mean germination time (MGT) were recorded during 10 days. Results: The results indicated that germination was significantly reduced in all species with the increase in NaCl concentrations. However, significant interspecific variation for salt tolerance was observed. The greatest variability in tolerance was observed at moderate salt stress (200 mM of NaCl) and the decrease in germination appeared to be more accentuated in A. cyanophylla and A. cyclops. Although, A. raddiana, remains the most interesting, it preserved the highest percentage (GC = 80%) and velocity of germination in all species studied in this study, even in the high salt levels. This species exhibited a particular adaptability to salt environment, at least at this stage in the life cycle and could be recommended for plantation establishment in salt affected areas. On the other hand, when ungerminated seeds were transferred from NaCl treatments to distilled water, they recovered largely their germination without a lag period and with high speed. This indicated that the germination inhibition was related to a reversible osmotic stress that induced dormancy rather than specific ion toxicity. Conclusion: This ability to germinate after exposure to higher concentrations of NaCl suggests that studied species, especially the most tolerant could be able to germinate under the salt affected soils and could be utilized for the rehabilitation of damaged arid zones.
  Hallouti Ayoub , Zahidi Abdelaziz , Lamharchi El Hassane , Boubaker Hassan , El Mousadik Abdelhamid and Ait Benaoumar Abdellah
  Background and Objective: Ceratitis capitata is considered as one of the most economically destructive pest. Several alternatives have been used but efficacy remains limited. The objective consists in the search for other natural enemies such as parasitoids and evaluate parasitism rate of larval stages from infested fruits by medfly collected in argane forest. Materials and Methods: About 400 fly larvae per sample and per locality were isolated and placed in breeding boxes at optimal temperature until the emergence of adults. Number of Ceratitis adults and parasitism rate have been observed. Morphological and molecular identification using COI-F/COI-R primers of collected parasitoids were also occurred. Results: Morphological and molecular analyses confirmed the occurrence of a new haplotype of the larval parasitoid belonging to Psyttalia genus in argane forest. In addition, phylogenetic analyses revealed that these specimens were 92% (E-value = 2e-85) identical to Psyttalia lounsburyi. The presence of this parasitoid was noted in all studied localities with a variable parasitism rate which reached a value of 21.5% in the localities where agricultural activities are present. Conclusion: It can be concluded that this natural parasitoid could be native to argane forest and will be used as biological control agent against Ceratitis capitata and Bactrocera oleae in Morocco. Also in Mediterranean region which constitute a handicap to the extension of biological agriculture of fruit trees.
  Lamharchi El Hassane , Zahidi AbdelAziz , Hallouti Ayoub , Akchour Abdellah , El Finti aissam , Boubaker Hassan and El Mousadik Abdelhamid
  Background and Objective: The California Red Scale (CRS) is a serious pest of citrus in many parts of the world. Different control methods have been adopted, but their effectiveness remains limited. The objective was to isolate fungi from the CRS corpses and determine the efficacy of two isolated strains, Aspergilllus flavus and Fusarium oxysporum against different CRS developmental stages. Materials and Methods: Fungal isolates obtained from RSC corpses were identified by studying their macroscopic and microscopic aspects. Mycelial biomass was used for genomic DNA extraction according to kit protocol. Polymerase Chain Reaction (PCR) with specific primers on a partial sequence was used for each fungal isolate. Each PCR product was sequenced in the forward and reverse directions using an Applied Biosystem ABI 3130×l sequencer. Results: The result of the study informs that most isolated strains belonged to five genera of Aspergillus, Alternaria, Fusarium, Rhizopus and Cladosporium. At concentration of 106 conidia mL1, pre-nymph male and adult male under shield showed greater mortality ranging from 84.02-93%, but for female stages, this rate did not exceed 58%. Adult males, male pre-pupae and mobile larvae (68.3-100%) are more affected by Fusarium than Aspergillus strain. Conclusion: It can be concluded that the two entomopathogenic fungi tested can be considered as good candidates for biological control of California Red Scale (CRS). Therefore, they will be used as microbial agents in Integrated Pest Management (IPM) strategies.
  Askarne Latifa , Talibi Idriss , Boubaker Hassan , Serghini Mohammed Amine , Boudyach El Hassane and Ait Ben Aoumar Abdellah
  Control of Citrus blue mold, caused by Penicillium italicum, has been accomplished by postharvest application of synthetic fungicides. However, the development of resistant fungal strains and increasing public concern over food safety and the environment are driving a search for alternative disease control strategies. In vitro trials were conducted to evaluate the effect of several organic acid and salt compounds on mycelial growth, sporulation, spore germination and germ tube elongation of Penicillium italicum. Among 28 tested compounds, sodium carbonate, ammonium carbonate, copper sulfate, EDTA and sodium metabisulfite completely inhibited mycelial growth and sporulation of Penicillium italicum at only 0.02 M. The lowest Minimum Inhibitory Concentration (MIC), Minimum Fungicidal Concentration (MFC) and EC50 values were recorded in sodium metabisulfite treatment. All tested compounds, except Nicotinic acid, strongly inhibited spore germination and germ tube elongation in a dose-dependent manner. Results from this study provide an important basis for further study into the uses of salt compounds for the control of blue mold of Citrus fruit under semi-commercial conditions.
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