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Articles by Bo Yu
Total Records ( 3 ) for Bo Yu
  Bo Yu , Huojun Cao , Chunjiang Zhao , Changxin Wu and Xuemei Deng
  Map-based positional cloning of Drosophila melanogaster genes is hampered by both the time-consuming, labor-intensive, error-prone nature of traditional methods for genetic mapping-SNP mapping is costly and P element mapping is low-resolution for limit number of P elements. Here we used two strategies to locate a spontaneous mutant screened in our lab. One is P element mapping to map the mutant in a 33 k-interval on the right arm of the second chromosome; another is combination of P element mapping and a Single-Nucleotide Polymorphism (SNP) mapping together to map the mutant in a very small interval, about 5 kb, on the right arm of the second chromosome efficiently. At last, we guessed that the mutant phenotype maybe resulted from the overexpression of twist gene. Comparing the two strategies, the method combining the P insertion mapping and SNP mapping could ward off their drawbacks. Therefore, the strategy of combination of the two methods provides a rapid protocol for mapping mutations.
  Hongwei Li , Chunjiang Zhao , Haiyue Xu , Bo Yu and Changxin Wu
  Classic taxonomy of animal mainly depends on genetic, morphology, fossil and distribution studies, so the study aims at developing a method which can rapidly identify to what species unknown animals are closely related by comparison of genomic sequences. With more complete sequenced animal genomes available, genomic information will play a key role on classifying unknown animals. Here, we put forward a method how to rapidly identify unknown animals. The method includes: (1) selecting a vector which is convenient for cloning and sequencing; (2) digesting the total genomic DNA from unknown animals and the vector with the same two enzymes which have recognition sites on the vector; (3) ligating the digested DNA to the vector; (4) transforming the ligation products to competent cell; (5) selecting positive clones to sequence and blast with sequenced animal genomes; (6) then finding out the most close relative species to the unknown animals. In the paper we took a kind of unknown wild flies which captured in nature, for example, to display the method. By analysis of data from blast output, results showed that eleven query sequences (1, 2, 4, 5, 6, 7, 9, 10, 12, 13, 14) from unknown flies have the highest similarities with subject sequences on the D. ananassae genome, but only minor parts of No. 2, 4~7, 9, 10 and 12 sequences have similarities with subject sequences on eleven drosophila genomes and the unknown flies are the most closely related to D. ananassae in evolutionary contexts. it will be a promising power in rapidly identifying to what species unknown animals is closely related when more complete sequenced animal genomes are available .
  Qing Zhai , Xiao-Lan Bian and Bo Yu
  The administration of carbon tetrachloride (CCl4) to mice produced hepatotoxicity, showing a significant increase in the serum levels of alanine transaminase (ALT) and aspartate aminotransferase (AST). Mice pretreated with Jiang-Zhi-Li-Gan (JZLG, 100–900 mg/kg, p.o.), a domestic remedy for liver disease in Rui-Jin Hospital, showed a significant decrease in serum ALT and AST levels when compared to the group treated with CCl4 alone. The standard drug, bifendate (200 mg/kg, p.o.), also exhibited similar results. In the acute toxicity study, JZLG did not show any mortality up to a dose of 32 g/kg body weight. Based on the results obtained, it can be concluded that JZLG seems to possess hepatoprotective activity in mice. These results support the use of this prescription against chemical hepatic injury.
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