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Articles by Bo Wu
Total Records ( 2 ) for Bo Wu
  Zengbing Xu , Jianping Xuan , Tielin Sh , Bo Wu and Youmin Hu

This paper presents a novel intelligent diagnosis method based on multiple domain features, modified distance discrimination technique and improved fuzzy ARTMAP (IFAM). The method consists of three steps. To begin with, time-domain, frequency-domain and wavelet grey moments are extracted from the raw vibration signals to demonstrate the fault-related information. Then through the modified distance discrimination technique some salient features are selected from the original feature set. Finally, the optimal feature set is input into the IFAM incorporated with similarity based on the Yu’s norm in the classification phase to identify the different fault categories. The proposed method is applied to the fault diagnosis of rolling element bearing, and the test results show that the IFAM identify the fault categories of rolling element bearing more accurately and has a better diagnosis performance compared to the FAM. Furthermore, by the application of the bootstrap method to the diagnosis results it can testify that the IFAM has more capacity of reliability and robustness.

  Tomoya Saito , Manami Nishi , Muoy I. Lim , Bo Wu , Takuya Maeda , Hisayuki Hashimoto , Tsutomu Takeuchi , David S. Roos and Takashi Asai
  We previously reported a cytosolic pyruvate kinase (EC [EC] 0) from Toxoplasma gondii (TgPyKI) that differs from most eukaryotic pyruvate kinases in being regulated by glucose 6-phosphate rather than fructose 1,6-diphosphate. Another putative pyruvate kinase (TgPyKII) was identified from parasite genome, which exhibits 32% amino acid sequence identity to TgPyKI and retains pyruvate kinase signature motifs and amino acids essential for substrate binding and catalysis. Whereas TgPyKI is most closely related to plant/algal enzymes, phylogenetic analysis suggests a proteobacterial origin for TgPyKII. Enzymatic characterization of recombinant TgPyKII shows a high pH optimum at 8.5, and a preference for GDP as a phosphate recipient. Catalytic activity is independent of K+, and no allosteric or regulatory effects were observed in the presence of fructose 1,6-diphosphate, fructose 2,6-diphosphate, glucose 6-phosphate, ribose 5-phosphate, AMP, or ATP. Unlike TgPyKI, native TgPyKII activity was exclusively associated with the membranous fraction of a T. gondii tachyzoite lysate. TgPyKII possesses a long N-terminal extension containing five putative start codons before the conserved region and localizes to both apicoplast and mitochondrion by immunofluorescence assay using native antibody and fluorescent protein fusion to the N-terminal extension. Further deletional and site-directed mutagenesis suggests that a translation product from 1st Met is responsible for the localization to the apicoplast, whereas one from 3rd Met is for the mitochondrion. This is the first study of a potential mitochondrial pyruvate kinase in any system.
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