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Articles by Bo WANG
Total Records ( 9 ) for Bo WANG
  Shi Shu , Cheng Xia , Hongyou Zhang , Zhaolei Sun , Jiannan Liu and Bo Wang
  Milk fever is an important metabolic disorder of dairy cows at calving and is characterized by hypocalcemia during the transition period. The aim of this study is to investigate novel changes in the plasma proteomics of cows with milk fever. Surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) was used for many field as a novel proteomics teachnology. So far, the plasma proteomics of milk fever has not been investigated using SELDI-TOF-MS. Plasma samples were obtained from twenty-one Holstein cows with milk fever (T) and fifty-nine Holstein cows without milk fever (C) at a dairy farm in Heilongjiang, China. Twenty-four differential peptide peaks in the plasma of T and C cows were isolated by SELDI-TOF-MS. Ten of these peaks were identified using the Swissport Protein Database. The peptide peaks represented ten unique proteins and showed significant alterations in their peaks as determined by analysis using the Wilcoxon Rank Sum Test. The four up-regulated proteins were identified as complement c3 frag, hepcidin, amyloid bata a4 protein, serum albumin frag and fibrinogen. Complement c3 frag and hepcidin regulate the inflammatory response. Amyloid beta a4 protein is involved in Alzheimer’s disease. Serum albumin frag acts as a transport protein. Fibrinogen beta chain participates in blood coagulation. The two down-regulated proteins were plasma protease c1 inhibitor frag and apolipoprotein a-2 which are associated with, respectively, blood coagulation and cardiovascular disease. The four proteins that were both up-regulated and down-regulated were fibrinogen alpha chain frag, neurosecretory protein vgf frag, serun amyloid a protein and cystatin-c. Based on SELDI-TOF-MS, identify novel plasma proteins that may be closely associates with milk fever in cows. These findings may reveal previously unidentified metabolic changes in cows with milk fever.
  Yongbin Lai , Bo Wang , Xiu Chen , Yinnan Yuan , Lei Zhong , Xing Qiao , Yuqi Zhang , Menghong Yuan , Junfeng Shu and Peng Wang
  Palm oil and rapeseed oil were transesterified with methanol using NaOH as catalyst to obtain Palm Methyl Ester (PME) and Rapeseed Methyl Ester (RME). To investigate the combustion characteristics of PME and RME, the chemical composition was analyzed by Gas Chromatography-Mass Spectrometry (GC-MS); fuel properties including density, kinematic viscosity, flash point, cold filter plugging point, sulfur content and copper strip corrosion were characterized; combustion experiment was performed in the furnace of Thermogravimetry-Differential Scanning Calorimetry (TG-DSC) simultaneous thermal analyzer. Combustion characteristic index was put forward for describing biodiesel combustion characteristic. The study showed that the biodiesel was mainly composed of Fatty Acid Methyl Esters (FAMEs): C14:0-C24:0, C16:1-C22:1, C18:2 and C18:3. The Saturated Fatty Acid Methyl Esters (SFAMEs) content of PME and RME were 35.86 and 14.69 wt.%, respectively. Biodiesel is oxygenated fuel had a good burnability. Combustion characteristic indexes of PME, RME were 4.97E-05 and 3.65E-05, respectively. The combustion characteristic had relation to chemical composition. The combustion characteristic of biodiesel was better with increasing SFAMEs length of carbon-chain, was poorer with increasing Unsaturated Fatty Acid Methyl Esters (UFAMEs) and unsaturated degree. The combustion characteristic of PME was better than that of RME.
  Hai-Hong Wang , Zhen Zhao , Bo Wang and Jie Yin
  The economic activity and assessment methods of the Mariculture Functional Zones (MFZ) in Qingdao are investigated. A System Dynamic (SD) model of the prediction and the utilization Effectiveness for Qingdao MFZ is established to provide decision-making for managers. A fishery impact assessment for the MFz is set up to estimate the total incomes and outcomes and thus the cause and effect feedback chart is obtained. Data are processed by statistical method and World Wind Java. The causal loop diagram is presented to express the feedbacks. Simulations are conducted to verify the validation of the model proposed based on Vensim.
  Linjie Wang , Li Li , Bo Wang and Hongping Zhang
  Insulin like Growth Factors (IGF-I and IGF-II) play an important role in regulation of skeletal muscle growth and act as mediator of growth hormone during muscle development and differentiation. In this study, the expression profiling of goat IGF-I and IGF-II genes was investigated in liver and skeletal muscle at six postnatal development stages (3, 15, 30, 60, 90 and 120 days) and two types of muscles (longissimus dorsi muscle, LD; triceps brachii muscle, TB) using relative real-time quantitative PCR. The results showed that IGF-I gene was expressed at the highest level in the liver with relatively high expression levels in spleen, skeletal muscle and heart. IGF-II gene was predominantly expressed in spleen, followed by liver and longissimus dorsi muscle, whereas expression in heart was relatively weak. IGF-I and IGF-II genes had different expression profiles during the postnatal period of the goat liver and skeletal muscle development. Moreover, IGF-I was expressed at higher levels in triceps brachii muscles compared with longissimus dorsi muscles. The present study provided the comprehensive understanding of IGF-I and IGF-II expression during liver and muscle development and laid the basis for further studies on their roles in goat liver and skeletal muscle development.
  Cheng Xia , Shi Shu , Bo Wang , Chuang Xu , Hong-You Zhang , Ling Wu and Jia-San Zheng
  This study assessed the effect of anionic salts in the prevention of hypocalcaemia in dairy cows. Fifty multiparous Holstein cows were randomly divided into 5 groups: Control group (C) and 4 treatment (T1-T4) groups. The T groups were fed different doses of anionic salts added to their diets from day 21 pre-calving and The Dietary Cation-Anion Difference (DCAD) was 85 mEq kg-1 DM (C), -30 mEq kg-1 DM (T1), -80 mEq kg-1 DM (T2), -130 mEq kg-1 DM (T3) and -180 mEq kg-1 DM (T4). Plasma Ca2+, Hydroxyproline (HYP), 1,25-Dihydroxyvitamin D3 (DHVD), Parathyroid Hormone (PTH) and pH were determined during the transition period. The results revealed that there was a relatively high incidence of hypocalcaemia (80%) in the C group compared with the T groups (35-65%). Furthermore, plasma concentrations of Ca2+ and HYP in the T groups were higher than those of the C group at calving (p<0.05) and plasma pH value (p<0.05) and PTH concentration (p<0.05 or p>0.05) in the T groups were lower than those of the C group at calving. These results suggest that anionic salts may induce metabolic acidosis and reduce the incidence of hypocalcaemia. Compared to all groups, cows in the T3 group had the lowest incidence of hypocalcaemia and plasma PTH concentrations (p<0.05) and the highest plasma Ca (p<0.05) and HYP (p<0.05) concentrations at calving indicating that cows fed a DCAD diet of -130 meq kg-1 DM prior to calving may efficiently activate Ca homeostatic mechanisms. In conclusion, anionic salts prior to calving may enhance Ca homeostasis and reduce the incidence of hypocalcaemia in dairy cows.
  Bo Wang , Shiwei He and Pengwei Lin
  With the development of railway freight station, there must be some optimization problems of resources allocation under different situations. Different layout optimization objectives which aimed at minimizing the total cost based on the different situations are put forward under the constraints of freight demand, capacity of rail freight station and scale of the whole station. SoftwareILOG is used to solve this mixed integrate programming model. This study showed that the optimization ideas and method of this study can provide basis for decision making of freight station optimization problem.
  Bo WANG , Tao LAI , Qi-Wei HUANG , Xing-Ming YANG and Qi-Rong SHEN
  Endogenous hormones play an important role in the growth and development of roots. The objective of this research was to study the effect of four types of N fertilizers on the root growth of strawberry (Fragaria ananassa Duchesne) and the endogenous enzymes of indole-3-acetic acid (IAA), abscisic acid (ABA), and isopentenyl adenosine (iPA) in its roots and leaves using enzyme-linked immunosorbent assay. Application of all types of N fertilizers significantly depressed (P ≤ 0.05) root growth at 20 d after transplanting. Application of organic-inorganic fertilizer (OIF) as basal fertilizer had a significant negative effect (P ≤0.05) on root growth. The application of OIF and urea lowered the lateral root frequency in strawberry plants at 60 d (P ≤0.05) compared with the application of two organic fertilizers (OFA and OFB) and the control (CK). With the fertilizer treatments, there were the same concentrations of IAA and ABA in both roots and leaves at the initial growth stage (20 d), lower levels of IAA and ABA at the later stage (60 d), and higher iPA levels at all seedling stages as compared to those of CK. Thus, changes in the concentrations of endogenous phytohormones in strawberry plants could be responsible for the morphological changes of roots due to fertilization.
  Xu Zhang , Qin-jian Xie , Xin Wang , Bo Wang and Hong-yu Li
  Acidithiobacillus ferrooxidans is a Gram-negative, chemolithoautotrophic bacterium involved in metal bioleaching. It is used for the extraction of coarse medical realgar, which is converted into an aqueous solution. To prove its feasibility as an anticancer drug candidate, extracted realgar (ER/Af) was evaluated for its antitumor activities both in vitro and in vivo. In cytotoxicity tests, ER/Af displayed significant inhibition on cell proliferation of HepG2, SMMC7721, and H22 cells in a time and dose dependent manner. Remarkable tumor growth inhibition and survival time prolongation effects, along with no obvious toxicity, were observed in antitumor experiments against H22 cell-bearing mice. Apoptosis induction was also confirmed as one of the mechanisms involved in the efficacy of ER/Af both in vitro and in vivo. The most important observation is that ER/Af showed high selective affinity to tumor tissues with about eight-fold higher arsenic accumulations at the tumor site of mice than those of the arsenic trioxide (ATO)-treated group at the same dose (57.8 ± 3.34 μg/g dry tissue vs. 7.6 ± 0.88 μg/g dry tissue). In conclusion, A. ferrooxidans could be successfully used for the extraction of realgar and ER/Af was proved to be a promising anticancer drug candidate, which is valuable for further study and clinical trials.
  Mohd W. Nasser , Jharna Datta , Gerard Nuovo , Huban Kutay , Tasneem Motiwala , Sarmila Majumder , Bo Wang , Sau Suster , Samson T. Jacob and Kalpana Ghoshal
  Micro-RNAs are ∼21–25-nucleotide-long noncoding RNAs that regulate gene expression primarily at the post-transcriptional level in animals. Here, we report that micro-RNA-1 (miR-1), abundant in the cardiac and smooth muscles, is expressed in the lung and is down-regulated in human primary lung cancer tissues and cell lines. In situ hybridization demonstrated localization of miR-1 in bronchial epithelial cells. The tumor suppressor C/EBPα, frequently suppressed in lung cancer, reactivated miR-1 expression in the lung cancer cells. Repressed miR-1 was also activated in lung cancer cells upon treatment with a histone deacetylase inhibitor. These observations led us to examine the antitumorigenic potential of miR-1 in lung cancer cells. Expression of miR-1 in nonexpressing A549 and H1299 cells reversed their tumorigenic properties, such as growth, replication potential, motility/migration, clonogenic survival, and tumor formation in nude mice. Exogenous miR-1 significantly reduced expression of oncogenic targets, such as MET, a receptor tyrosine kinase, and Pim-1, a Ser/Thr kinase, frequently up-regulated in lung cancer. Similarly, the levels of two additional targets, FoxP1, a transcription factor with oncogeneic property, and HDAC4 that represses differentiation-promoting genes, were reduced in miR-1-expressing cells. Conversely, depletion of miR-1 facilitated N417 cell growth with concomitant elevation of these targets. Further, ectopic miR-1 induced apoptosis in A549 cells in response to the potent anticancer drug doxorubicin. Enhanced activation of caspases 3 and 7, cleavage of their substrate PARP-1, and depletion of anti-apoptotic Mcl-1 contributed to the sensitivity of miR-1-expressing cells to doxorubicin. Thus, miR-1 has potential therapeutic application against lung cancers.
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