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| Articles
by
Bing Han |
Total Records (
3 ) for
Bing Han |
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Bing Han
,
Hui-Ling Zhang
,
Li Zeng
,
Bin Yang
,
Reyanggu Abula
,
Xiao-Li Xu
and
Yong Chen
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The beta-adrenergic receptors (β-AR) are a class of metabotropic G protein-coupled receptors that responsible for the muscle growth, fat deposition or lactation characteristics of domestic animals. The aim of this article was to scan nucleotide mutations in partial coding region of β2-AR gene on cattle. In this study, the polymorphisms of β2-AR gene on cattle were detected by methods of Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) and DNA sequencing in two cattle breeds in China, Xinjiang Brown cattle and Chinese Holstein cows. The results revealed that there were two genotypes and homozygote variant was not found in the two breeds. The genotypic frequencies at the Sma I locus were significantly different between the two breeds. The wild-type allele was ascendancy in the two breeds but its allele frequency was distinctly lower in the Chinese Holstein cows than that in the Xinjiang Brown cattle. DNA alignment results showed that three single nucleotide polymorphisms (SNPs), C11A, G53A and C129T were found in 5-coding region of β2-AR gene. The C11A mutation resulted in disappearance of Sma I cleavage site. In addition, the C11A and G53A mutations caused two amino acid residues replacement at the 4th and 18th of β2-AR. However, the C129T mutation was a synonymous one. In conclusion, the heterozygous frequency in Chinese Holstein cows was obviously higher than that in the Xinjiang Brown cattle. The Sma I locus would be a potential genetic marker for lactation performance of cattle. |
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Bao-Sheng Liu
,
Gui-Guan Li
,
Zhan-Qiao Yu
,
Bing Han
and
Ri-Jun Zhang
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In order to pre-evaluate the p ossibility of bacteriocin resistance
when utilizing a bacteriocin complex against pathogenic bacteria, Staphylococcus
aureus was used to develop a resistant variant by stepwise method. The spontaneous
frequency of S. aureus resistance to bacteriocin complex was about 10-5.
The stability of resistant variant was confirmed by hundred successive transfers
without bacteriocin complex stress. The growth rate of resistant variant was
similar as that of its original strain. Interestingly in co-culture system,
even inoculated with 90% of resistant variant, the resistant variant was cleared
out by wild strain in eight consecutive transfers. When treated with either
antibiotics or nisin, the resistant variant was more susceptible than the wild
strain to all tested antimicrobial agents except chloramphenicol. However, only
the tetracycline and chloramphenicol tested groups showed statistically significant
increase and decrease of antibacterial potency (p<0.05), respectively. The
cellular membrane fatty acid composition of resistant variant was similar to
that of wild strain when cultured without bacteriocin complex suggested that
the alterations of membrane fatty acid composition in resistant variants might
not be the primary defense against bacteriocin. It is proposed that the bacteriocin
complex can be used in animal disease preventions and therapeutics as an alternative
or complement to traditional antibiotics. |
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Yaohui Shi
,
Bing Han
,
Xiaofeng Yu
,
Shaochun Qu
and
Dayuan Sui
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Context: Panax ginseng C. A. Mey (Araliaceae) has been widely used in clinic for treatment of cardiovascular diseases in China. Ginsenoside Rb3 is the main chemical component of Panax ginseng. Objective: The aim of this study was to evaluate the effect of ginsenoside Rb3 on myocardial ischemia-reperfusion injury in rats. Methods: Sprague−Dawley rats were orally treated with Rb3 (5, 10 or 20 mg/kg) daily for 3 days followed by subjecting to left anterior descending coronary artery ligation for 30 min and reperfusion for 24 h. Results: This study showed that ginsenoside Rb3 treatment resulted in a reduction in myocardial infarct size. Ginsenoside Rb3 significantly attenuated the changes of creatine kinase activity and lactate dehydrogenase activity. The cardioprotective effect of ginsenoside Rb3 was further confirmed by histopathological examination. Ginsenoside Rb3 alleviated the increase of malondialdehyde content and the decrease of superoxide dismutase activity in left ventricle. Treatment with ginsenoside Rb3 also decreased plasma endothelin and angiotensin II levels. Conclusion: These findings suggested that ginsenoside Rb3 possesses the effect against myocardial IR injury and the underlying mechanism is related to its antioxidant activity and microcirculatory improvement. |
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