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Articles by Biman Kumar Dutta
Total Records ( 2 ) for Biman Kumar Dutta
  Arunava Das , Yahya Mazumder , Biman Kumar Dutta , Bibek Ranjan Shome , Komal Molla Bujarbaruah and Ashok Kumar
  This paper reports the investigation of necrotic enteritis (NE) in six broiler chickens of age two to three weeks old, died in Jowai poultry farm, Meghalaya, India. Initially, scanning electron microscopy (SEM) was performed to observe the morphological changes within the intestine. Intestinal contents and liver samples from dead chicken were investigated for isolation of bacteria and their virulence determinant. The SEM analysis of infected intestine revealed massive necrosis and complete destruction of the intestinal villi within the intestinal mucosa. Bacterial isolation confirmed the causative agent as C. perfringens in NE. All the isolates harboured single and double plasmid deoxyribonucleic acid with identical 45.2kb common plasmid. In polymerase chain reaction (PCR) assay all 10 clinical isolates harboured alpha toxin gene (cpa) of C. perfringens, however, four isolates also carried additional beta2 toxin gene (cpb2). None of the isolates were positive for beta, epsilon, iota and enterotoxin genes. PCR analysis revealed that all isolates derived from NE belonged to C. perfringens type A. The partial cpa gene sequence analysis showed 97.6 to 100% homology among the C. perfringens isolates. The study confirmed that C. perfringens type A is the most predominant one associated with necrotic enteritis in broiler chickens in Meghalaya, India and the alpha toxin (CPA) might play a significant role in the pathogenesis of the disease in broiler chicken.
  Kholhring Lalchhandama , Bishnupada Roy and Biman Kumar Dutta
  The anthelmintic activity of the ethanol extract of Acacia oxyphylla Graham ex Bentham (Mimosaceae) stem bark was tested against Ascaridia galli Schrank (Nematoda), the intestinal roundworm of domestic fowl. Different concentrations of the extract (0.5, 1, 2, 5, 10 and 20 mg/mL) were prepared in 0.9% phosphate buffered saline (PBS) with 1% dimethylsulfoxide (DMSO). In vitro treatment of the adult roundworms indicated concentration-dependent efficacy of the plant extract. Similar concentrations of a broad-spectrum antiparasitic drug, albendazole, were used as a standard reference. Control experiments consisted of nematodes maintained in 0.9% PBS with 1% DMSO. Albendazole was significantly effective (P < 0.05) at all concentrations tested in causing mortality of A. galli. However, the plant extract showed concentration-dependent efficacy only at the concentrations of 2, 5, 10, and 20 mg/mL. In order to ascertain the anthelmintic effect, scanning electron microscopy was performed, which indicated devastating structural alterations on the fine topography of A. galli treated with 20 mg/mL of the plant extract, when compared with that of the control specimen. Severe shrinkage of the cuticle, loosening and collapse of the lips, and extensive irregular wrinkles all over the body surface were very distinct on the plant extract-treated nematode. Moreover, high magnification of the cuticle revealed formation of a number of small swellings or blebs, which apparently marked the initiation of disintegration of the entire cuticle.
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