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Articles by Billy Kepel
Total Records ( 2 ) for Billy Kepel
  Fatimawali , Billy Kepel and Trina Ekawati Tallei
  Background and Objective: Efforts at organic mercury detoxification can be carried out using resistant bacteria that can live in an environment contaminated with the compound. This study aimed at isolating and identifying resistant bacteria from mercury-contaminated environments and analysing their ability to detoxify organic mercury. Materials and Methods: Soil samples were obtained from 3 gold processing locations that make use of mercury in Tanoyan Village, Bolaang Mongondow district, North Sulawesi province. The identification was carried out on the mercury-resistant bacteria through morphological and molecular tests. Bacteria which were highly resistant to mercury were examined for their ability to detoxify phenyl mercury (organic mercury). Results: The study showed that 8 mercury-resistant bacterial colonies could be isolated from the three soil samples. The bacteria were able to grow in LB broth containing 10 mg L1 of phenyl mercury. Four isolates (AA, BB, CC and DD) were even able to grow in 40 mg L1 of phenyl mercury. According to the identification tests, those bacteria were Pseudomonas sp. (AA, DD), Pseudomonas aeruginosa (BB) and Proteus mirabilis (CC). Testing of organic mercury against isolates of bacteria which are highly resistant to it in order to determine their detoxification capacity revealed that all four isolates could reduce levels of the compound in media, based on the results, starting from the highest was Pseudomonas sp. 74.99%, then Pseudomonas aeruginosa 60.23% and Proteus mirabilis 47.59% after 24 h of incubation. Conclusion: The study suggested that there are four bacteria that have potentials to remediate organic mercury contamination sites.
  Billy Kepel , Widdhi Bodhi , Fatimawali and Trina Ekawati Tallei
  Background and Objective: North Sulawesi is rich in minerals, among them gold is also present. The gold mining in the Buyat area produces heavy metal waste which can pollute the environment, among others is arsenic. Arsenic is a heavy metal that is very toxic to humans, so an agent is needed for the remediation process. The aim of this study was to isolate and identify arsenic-resistant bacteria from the Buyat estuary and beach to analyze the isolates’ ability to detoxify arsenic. Materials and Methods: Soil sediment samples were obtained from Buyat estuary and beach in North Sulawesi. Isolation of arsenic-resistant bacteria was carried out by growing the samples in LB broth media containing 100, 500 and 1000 ppm arsenite. Indentification of arsenic-resistant bacteria was carried out by microbiological, biochemical and biomolecular analysis. The ability to detoxify arsenite was analyzed by CVAFS. Results: The study showed that there were 4 isolates of arsenic-resistant bacteria isolated from the soil samples. All isolates are rod-shaped, Gram-negative and non-motile bacteria. BLAST results showed that isolates A was Stenotrophomonas sp., isolate B was Stenotrophomonas maltophilia, isolate C was Pseudomonas sp. and isolate D was Pseudomonas putida. All isolates reduced the levels of arsenic in media by almost 100% within 72 h. Conclusion: The study suggested that Stenotrophomonas sp., S. maltophilia, Pseudomonas sp. and P. putida had the potentials to be used in the bioremediation of arsenic.
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