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Articles by Bidhan Roy
Total Records ( 2 ) for Bidhan Roy
  Bidhan Roy and Asit B. Mandal
  The aim of this experiment was to induce profused microtillering of androgenic plantlets of elite indica rice (Oryza sativa L.) variety IR 72. Two microtillers were inoculated in each culture tube containing different medium and growth regulators. Various combinations of growth regulators were also used with each culture medium. Microtillering ability were noted on 45th day after inoculation of microtillers. MS medium with 4-8 mg L-1 of kinetin showed very fast microshooting with maximum of 94.7 shootlets per cultured shoot within 45 days of inoculation. Conversely, BAP at high dose (4-8 mg L-1) was found to exert inhibitory effects on shoot development, but induced large number of dormant embryos and pro-embryos. The caulogenic shoots that emerged from the base of the explants were successfully induced root on MS supplemented with 1 mg L-1 IAA. Evaluation of clonal fidelity of the microshoots through profiling of isozyme in respect of peroxidase and malate dehydrogenase and RAPD analysis was done to confirm true-to-type nature of the micropropagated plants. Microtillering and subsequent whole plant development may offer ample scope to develop large number of clones from a stock of choice for in vitro conservation, characterization and screening for diverse characters of agronomic importance. This approach may have far reaching consequences in transgenic development especially through direct bombardment of miniature shootlets en route biolistic and their subsequent multiplication en masse.
  Bidhan Roy , S.K. Noren , Asit B. Mandal and Asit K. Basu
  Abiotic stresses have become an integral part of crop production. One or other persist either in soil or in atmosphere. With the ultimate goal to raise the crop plants with better suitability towards rapidly changing environmental inputs, intense efforts are needed employing physiological, biochemical and molecular tools to improve tolerance ability under abiotic stresses. Attempts have been taken by plant breeders to develop tolerant varieties of different crops for specific abiotic stress. Appreciable improvement also has been done by the molecular biologists regarding to perturbations in gene expression and protein during stress. Employing transgenic technology, functional validation of various target genes involve in diverse processes, such as signaling, transcription, ion homeostasis, antioxidant defense etc. for enhanced abiotic stress tolerance has been attempted in various model system and some of them have been extended to crop plants. The information in the areas of gene and genetic engineering for improvement of crop plants against abiotic stresses are lying unorganized in different articles of journals and edited books. This information has been compiled in this review article in organized way with up-to-date citations, which will provide comprehensive literatures of recent advances.
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