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Articles by Benthotage Chamara Jayasankha De Silva
Total Records ( 2 ) for Benthotage Chamara Jayasankha De Silva
  Mitchell Wendt , Benthotage Chamara Jayasankha De Silva and Gang-Joon Heo
  Background and Objectives: A gram-negative zoonotic bacterium, Pseudomonas aeruginosa, is well-known for its antimicrobial resistance and ubiquity in nature. The current study aimed to examine pet turtle-borne P. aeruginosa for its virulence determinants and the antimicrobial resistance. Methodology: Twenty-four turtles purchased from pet shops and online markets in Korea were examined to determine whether they excreted P. aeruginosa. Presumptive P. aeruginosa was isolated from the fecal samples of pet turtles by selective media incubation and verified with biochemical testing. Seventeen isolates were genetically characterized by 16S rRNA sequencing and confirmed as P. aeruginosa. These strains were further tested for antimicrobial resistance by disk diffusion test and PCR assays were conducted to detect virulence genes. Results: All tested strains showed susceptibility to ciprofloxacin and ofloxacin but were completely resistant to amoxicillin, colistin, streptomycin, cephalothin, trimethoprim-sulfamethoxazole, chloramphenicol, imipenem, cefoxitin and nalidixic acid. Against gentamycin, tetracycline, ceftriaxone and amikacin, some strains showed complete resistance while some were intermediate resistant. The PCR assay detected the presence of virulence genes, toxA (100%), lasB (100%) and exoS (53%), which aid in pathogenicity against humans. Conclusion: All the results indicated that the pet turtles pose a potential public health risk due to prospective zoonotic P. aeruginosa infection.
  Sudu Hakuruge Madusha Pramud Wimalasena , Gee-Wook Shin , Hansani Nilupama Kumari Senarath Pathirana , Benthotage Chamara Jayasankha De Silva , Sabrina Hossain and Gang-Joon Heo
  Background and Objective: Drug resistance in bacteria is a challenge both in human and veterinary medicine. This study was conducted to characterize quinolone resistant determinants in Morganella morganii isolated from pet turtles. Materials and Methods: Antimicrobial susceptibility of twenty-two M. morganii isolates against nalidixic acid, ciprofloxacin, ofloxacin and levofloxacin was examined by disk diffusion assay and the Minimum Inhibitory Concentration (MIC). Substitutions of the Quinolone Resistance Determining Region (QRDR) and Plasmid Mediated Quinolone Resistance (PMQR) genes were detected using conventional PCR assays and sequencing. Results: Three isolates were resistant to the all tested quinolones and one isolate was resistant only to nalidixic acid. In QRDR substitution analysis, three isolates displayed the Ser463Ala, Ser464Tyr and novel Glu466Asp substitutions in gyrB and the Ser80Ile substitution in parC. Two isolates displayed only Ser463Ala substitution in gyrB. The unique PMQR gene detected was qnrD, which was found in 59% of the isolates. The aac-(6’)-Ib-cr gene variant was identified in 50% of the isolates. In addition, neighbor-joining phylogenetic tree derived using gyrB gene sequences exhibited two distinct clads comprising, first; present study isolates with a quinolone-resistant isolate of human clinical origin and second; isolates of environmental origin. Conclusions: All results suggest healthy pet turtles might serve as a potential reservoir for quinolone-resistant M. morganii due to the high prevalence of PMQR determinants, especially, qnrD and target gene alterations in QRDR together with a novel mutation in gyrB.
 
 
 
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