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Articles by Bao Wen-Bin
Total Records ( 6 ) for Bao Wen-Bin
  Bao Wen-Bin , Wu Sheng-Long , Zhang Hong-Xia , Luan De-Qin , H.H. Musa and Chen Guo-Hong
  Sexes of cranes cannot be distinguished visually due to the morphological similarities between male and female. To facilitate the protection program for the endangered birds, red-crowned cranes in particular, genomic DNA was extracted from feather samples and a PCR-based sexing method was established using three primer combinations of avian sex-specific EE0.6 sequence. The specificity of the PCR was confirmed by amplifying genomic DNA from 22 known-sex cranes of seven species. After 30 cycles of amplification, easily distinguishable single bands for males and two bands for females were displayed by agarose gel electrophoresis. The sexes of 50 unknown-sex cranes of seven species were successfully identified using the PCR. The noninvasive PCR-based sexing method can not only facilitate the human-assisted breeding of cranes, but also provide useful reference for accurate sexing of other endangered birds.
  Wu Sheng-Long , H.H. Musa , Bao Wen-Bin , Wang Ke-Hua , Zhu Guo-Qian and Chen Guo-Hong
  The aim of this experiment was to investigate the effect of mutation in apoVLDL-II (apo very low density lipoprotein II) gene on meat quality traits of genetically fat (Anka) and lean (Rugao) chicken breeds. Meat quality traits such as Water Holding Capacity (WHC), pH, Color Density (CD) and tenderness (Shear value (kg cm 2)) were analyzed from breast muscle. Polymerase chain reaction single strand conformation polymorphism (PCR-SSCP) technique was developed to analyze a 280 bp region of the apoVLDL-II gene (exon 4). The average gene frequency of two breeds estimated was found to be 0.076±0.008 for allele A and 0.932±0.008 for allele B. The effective number of allele, gene diversity and Shannon`s Information index were 1.1642±0.0195, 0.1408±0.0144 and 0.2691±0.0212, respectively. Mutation in the apoVLDL-II gene was found to significantly (p<0.05) affect meat tenderness, whereas no significant effect was observed on OD, pH and WHC.
  Ji Ting , Yin Ling , Liu Min , Bao Wen-bin and Chen Guo-hong
  Analysing genetic diversity of Apis cerana cerana and Apis mellifera ligustica in China could provide a theoretical foundation for the research on the conservation and reasonable utilization of honey bee populations, but there have not public reports on this topic by using microsatellite markers until now. Genetic diversity of Changbaishan bee (Apis cerana cerana) and Pinghu royal jelly bee (Apis mellifera ligustica) in China were evaluated with 21 microstaellite loci, the genetic variability within breeds and genetic differentiation between breeds were estimated, the results showed that in 21 microstaellite loci, 171 alleles were found, the number of alleles per locus ranged from 3-13, the average expected heterozygosity and PIC of all loci were 0.7175 and 0.6755, respectively. Mean numbers of alleles of Changbaishan bee and Pinghu royal jelly bee were 1.86 and 6.27, the average of genetic differentiation measured as FST value, was 51.9% (p<0.001) and all loci contributed significantly (p<0.001) to this differentiation. It can also, be seen that the deficit of heterozygotes was very high (0.574) (p<0.001). Reynolds’ distance values between 2 populations were 0.734.
  Yin Ling , Ji Ting , Liu Min , Bao Wen-Bin and Chen Guo-Hong
  Genetic diversity and the relationship between genetic distance and geographical distance of 6 Apis cerana cerana populations in China were evaluated with 11 microstaellite loci, the results showed that the number of alleles per locus ranged from 7-21, the average expected heterozygosity and PIC of all loci were 0.7339 and 0.7082, respectively. Average number of alleles/locus was ranged from 2.3636 in Guangxi bee and Jiling bee to 6.4545 in Zhejiang bee. The overall expected heterozygosity of 6 Apis cerana cerana populations was 0.7339±0.0344, all populations showed relative large heterozygosity. In the whole population, the average of genetic differentiation among population was 32.4% (p<0.001). The geographical elements may own to the close relationship for particular population pairs, however, the equation FST/(1-FST) = -1.5904 + 1.0604 ln (d) and the result from Mantel’s test (p = 0.130) did not provide enough support for a significant correlation between the genetic and geographical pair wise distances.
  Shu Jing-Ting , Bao Wen-Bin , Cheng Jin-Hua and Chen Guo-Hong
  The genomes of 500 individuals in F2 population of Recessive White Chicken and Xianju Chicken were screened using 26 microsatellite markers. The correlation between microsatellite markers and meat quality traits in chickens were analyzed. The results showed that: ADL212 on chromosome 2 was significantly associated with pH value and tenderness. MCW004 on chromosome 3 and MCW223 on chromosome 5 were significantly associated with meat color. Four microsatellite markers were significantly associated with water lost rate, they were: MCW264 and ADL212 on chromosome 2, MCW223 on chromosome 5 and ADL211 on chromosome 9.
  Ji Ting , Yin Ling , Liu Min , Bao Wen-Bin and Chen Guo-Hong
  Genetic diversity of Pinghu royal jelly bee (higher royal jelly producing bee) and Suwang No.1 bee (lower royal jelly producing bee) were evaluated with 23 microstaellite loci, the genetic variability within breeds and genetic differentiation between breeds were estimated, the results showed that in 23 microstaellite loci, 211 alleles were found, the number of alleles per locus ranged from 3-14, the average expected heterozygosity and PIC of all loci were 0.6894 and 0.6560, respectively. Mean numbers of alleles of Pinghu royal jelly bee and Suwang No.1 bee were 6.65 and 6.13, inbreeding index (FST), Reynolds' genetic distance and gene flow between two populations were 0.194, 0.2146 and 1.0447, respectively, these results indicated that the heterozygosity and the genetic diversity of 2 bee populations were very high.
 
 
 
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