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Articles by Bambang Ariyadi
Total Records ( 2 ) for Bambang Ariyadi
  J. Prastowo , O. Herawati , Bambang Ariyadi and Kurniasih
  Background and Objective: Ascaridia galli (A. galli) infection can depress the body weight and egg production of domestic chickens. Synthetic anthelmintics are usually used as a treatment but A. galli resistance to synthetic anthelmintics is rising. The aim of this research was to understand the effects of combinations of Areca catechu seed and Anredera cordifolia leaf on the number of eggs per gram (EPG) of feces, body weight, number of Ascaridia galli worms, histopathology and immunoexpression of CD4+lymphocytes in the intestine. Materials and Methods: Three groups of fifteen chickens infected with A. galli were used in this research. For seven days, group 1 was orally administered a combination of 2 mg kg–1 b.wt., of Areca catechu and 1 mg kg–1 b.wt., of Anredera cordifolia, group 2 was given a combination of 5 mg kg–1 b.wt., of Areca catechu and 2.5 mg kg–1 b.wt., of Anredera cordifolia and group 3 was not treated. This research had ethical clearance No. 00040/04/LPPT/IV/2017. On the 10th day, the chickens were euthanized and examined for the number of EPG of feces, body weight, number of A. galli, histopathology and immunoexpression of intestinal CD4+lymphocytes. The data were analyzed statistically and the histopathological changes were analyzed descriptively. Results: The results showed that the combination of Areca catechu and Anredera cordifolia significantly reduced EPG of feces and eliminated A. galli in 60% of the chickens in group 1 but there was no significant difference in body weight among groups. In group 2, the alkaloids and saponins contained in Areca catechu and Anredera cordifolia display antagonistic activity. Thus, high doses do not reduce EPG levels in group 2. Immunohistochemistry showed that wound healing is improved in the treated groups. Conclusion: Ascaridia galli infection might be reduced by a combination of Areca catechu and Anredera cordifolia.
  Zainal Choiri , N. Danar Dono , Bambang Ariyadi , Chusnul Hanim , Ronny Martien and Zuprizal
  Objective: This study aimed to analyze the effect of nano-encapsulation of noni (Morinda citrifolia) fruit extract in drinking water on antibacterial activity and the morphology of intestinal villi in laying hens. Materials and Methods: The experiment was conducted in a completely randomized design consisting of 6 treatments and 5 replicates, with 12 chickens (20 weeks) in each replicate pen. The treatments were: P0 = Water without an additive (negative control), P1 = Water+50 mg/hen of tetracycline (positive control), P2 = Water+0.5% extract of noni fruit, P3 = Water+0.5% nano-encapsulation of noni fruit extract, P4 = Water+1% nano-encapsulation of noni fruit extract and P5 = Water+1.5% nano-encapsulation of noni fruit extract. At the end of the experiment (5 weeks), parameters included bacterial populations in the small intestine (Escherichia coli, Bacillus subtilis and Salmonella sp.) and the morphology of intestinal villi (villus height, villus width, crypt depth and villus height: Crypt depth) were observed. Data were analyzed statistically using one-way ANOVA. Orthogonal contrasts were used to analyze all data with significant differences. Results: The results showed that the addition of 1% nano-encapsulation of noni fruit extract in drinking water did not affect the morphology of the intestinal villi or the populations of Bacillus subtilis and Salmonella sp., but it reduced the population of Escherichia coli (p<0.05). Conclusion: Supplementation with nano-encapsulation of noni fruit extract in the drinking water of laying hens reduced colonization by Escherichia coli and might be used as an alternative to antibiotics in laying hens.
 
 
 
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