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Articles by Bahy R. Bakhit
Total Records ( 1 ) for Bahy R. Bakhit
  Bahy R. Bakhit and Bahaa E. Abdel-Fatah
  A six-generation analysis was employed to estimate the genetic parameters for plant height, number of branches and pods/plant, 100-seed weight and seed yield/plant. The six generations were derived from the cross between Misr-1 (tolerant) x Giza-2 (susceptible) to Orobanche. The results indicated that F1 exceeded its high performing parent for all studied traits except number of branches/plant. Inbreeding depression was positive and significant for all studied traits. Additive (d) gene effect was significant for all the studied traits except seed yield/plant; also, dominance gene effects (h) were significant for all the studied characters except for number of pods/plant and 100 seed weight. Two of the non-allelic gene interactions analysis (i) and (l), were significant for all characters except number of pods and 100-seed weight, while the (j) component was significant for seed yield/plant only. Bulked Segregant Analysis (BSA) with three molecular marker systems (RAPD, ISSR and SRAP) was performed to identify markers related to Orobanche tolerance. DNA was isolated from the two contrasting parents; F1, two contrasting groups of F2 plants and two contrasting groups of BC1 (F1 x Misr-1). Nine RAPD primers, ten ISSR primers and nine SRAP primers were used in this study. The results showed that six RAPD markers (OPA01 345 bp, OPA06 272 bp, OPA07 669bp, 385, 292 and OPB05 253 bp), six ISSR (HB 359 bp, 231 bp, HB08 521, 207 bp and HB11 509bp and 358 bp) and seven SRAP (SRAP1 354 bp, SRAP2 643bp and 200 bp, SRAP3 99 , 362, 169 bp and SRAP4 242 bp) were associated with Orobanche tolerance in faba bean These markers can be used in faba bean breeding for Orobanche resistance in marker-assisted selection programs.
 
 
 
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