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Articles by Bahram Kazemi
Total Records ( 4 ) for Bahram Kazemi
  Adel Haghighi Khiabanian Asl , Mojgan Bandehpour , Zarrin Sharifnia and Bahram Kazemi
  For consideration of the contamination of rainbow trout propagation and breeding centers in Iran to SVC (Spring Viremia of Carp), we prepared tissue samples from gills and parenchyma`s organs. Samples of paranchymatous organs and gills were taken and prepared for Molecular and pathological testing by standard methods. Internal wall of air bladder, kidney, liver, intestinal curves and skeletal muscles of rainbow trout with the symptoms of disease in these centers and considered the prepared samples by both molecular and pathological techniques. Thirty six samples had the typical pathological clinical signs and 14 samples were diagnosed positively by PCR method and 1 sample had doubtful result. The results of this study revealed the frequency of SVC virus in some centers of rainbow trout propagation and breeding in Iran and therefore, the control and diagnosis of disease is necessary. This is the first study of the SVC infection in rainbow trout in propagation and breeding centers in Iran and can be useful for this virus isolation and consideration of the severity of its disorders.
  Adel Haghighi Khiabanian Asl , Mehdi Soltani , Bahram Kazemi , Iraj Sohrabi Haghdoust and Isa Sharifpour
  The aim of this research was investigation on infection in reproducing and breeding centers of Rainbow trout in Iran by infectious Necrosis haematpoietic virus. We collected 100 samples from 100 reproducing and breeding centers of Rainbow trout of the Iran that showed clinical sings or were healthy in appearance in larva stage and finger link. Samples were tested by immunohistochemistry and Nested-PCR methods. By immunohistochemistry methods 35 samples and by PCR 43 samples were positive. There were 38 samples with clinical symptoms of Infectious Haematpoietic Necrosis. The results of this study show the dispersion of Infectious hematopoietic necrosis virus in some reproducing and breeding centers of Rainbow trout in the Iran. Also the result of two methods had acceptable overlapping.
  Ramin Pazoki , Bahram Kazemi , Mohammad Reza Nazari , Jafar Masoud and Mohammad Reza Akbari
  Two different sequences of Echinococcus granulosus Antigen B, (A major hydatid cyst fluid antigen), acquired from Gene Bank and amplified via RT-PCR reaction. The amplified fragments (HI, HII) cloned into pTZ57R vector by T/A cloning and subcloned into pGEMEX-1 expression vector. The subcloned genes were expressed by IPTG. To confirm the expression of subcloned genes, The SDS-PAGE performed and production of about 35 KDa recombinant fusion proteins were confirmed for either two cloned genes. The immunoreactivity of the recombinant fusion proteins were tested using double diffusion and immunoblotting. Both recombinant fusion proteins derived from lysate of transformed bacteria, were reactive for antibodies in serum of cystic hydatid patient.
  Bahram Kazemi , Sanaz Khalili-Tehrani , Mojgan Bandehpour , Negar Seyed and Nariman Mosaffa
  In the present research, bacterial DNA of pathogenic Escherichia coli was used as co-adjuvant; sheep blood total immunoglobulin G (IgG) was used as the antigen and complete Freund’s adjuvant (CFA) was the adjuvant. Rabbits were injected subcutaneously in the muscle with sheep blood total IgG ± adjuvant in a volume of 2 mL. Forty microgram IgG was suspended in saline with 20 μg soluble bacterial DNA or mixed with CFA with or without bacterial DNA. The study also included two control groups: One group was injected with 1 mL CFA plus 1 mL distilled water, the other group received no injections. Measurement of the proliferative responses by gel diffusion showed that priming with IgG plus bacterial DNA suspended in CFA leads to strong secondary responses to sheep IgG, indicating a strong synergistic interaction between the bacterial DNA and CFA.
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