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Articles by Bagher Yakhchali
Total Records ( 2 ) for Bagher Yakhchali
  Hassan Salimi , Parviz Owlia , Bagher Yakhchali and Abdolaziz Rastegar Lari
  Problem statement: Pseudomonas aeruginosa is an opportunistic pathogen that still develops life-threatening infections in patients with immunological system defects, like burn patients, especially in developing countries. Since this bacterium is naturally resistant to many drugs and is able to get resistance to all effective antibiotics, the infection with this organism is particularly problematic condition for patients. This study was design to evaluated drug susceptibility for determination of multidrug-resistant isolates and molecular epidemiology of P. aeruginosa colonization for investigation of the isolates routes in the burn unit of Shahid Motahhari Hospital in Tehran. Approach: About 127 clinical and 2 environmental P. aeruginosa isolates were collected during 6 months. All P. aeruginosa isolates were analyzed for drug susceptibility by disk diffusion method and molecular epidemiology assessment were done by RAPD-PCR analysis. Results: Drug susceptibility tests were shown high resistance for ceftizoxime (86.8%), aztreonam (80.6%), kanamycin (79.8%) and tetracycline (78.3%); furthermore, low resistance for some antibiotics like imipenem (30.2%), piperacillin/tazobactam (34.1%) and amikacin (41.1%) was showed. In addition, 42 multidrug-resistant P. aeruginosa isolates were recovered from clinical specimens and one isolate from environmental samples. Molecular typing revealed eight different profiles that include two profiles, RAPD1 and RAPD4, with environmental resource. The major RAPD profile was RAPD1 profile (n = 64, 50.4%), which includes 31 (72.1%) multidrug-resistant isolates with an environmental reservoir. Conclusion: In summery, we were found three different profiles for multidrug-resistant strains. Different RAPD profiles suggested the different resources of infection, two environmental resources were found, that one of them was multidrug-resistant strain. These findings highlighted the need for further attention to disinfection inanimate hospital environment and controlled contact between staff with patients to limit transfer of P. aeruginosa in this BU; moreover, use of some antimicrobial agents must be restricted due to existence of high resistance and using of combined effective antibiotics is recommend.
  Kambiz Morabbi Heravi , Fereshteh Eftekhar , Bagher Yakhchali and Fatemeh Tabandeh
  Lipase production in an indigenous lipolytic Bacillus sp. was detected in media containing Tributyrin-Tween 80 and Rhodamine B-Olive oil. The statistical Taguchi model was used to predict the optimum experimental conditions for bacterial growth and lipase production. Partial optimization was carried out for selection of salt base, oil, glucose, NH4Cl and yeast extract concentrations, inoculum density, pH and agitation. Maximum lipase activity was detected in the cell free supernatants of cultures grown in a medium containing 10 g L-1 yeast extract, 15 g L-1 NH4Cl, 3 g L-1 K2HPO4, 1 g L-1 KH2PO4, 0.1 g L-1 MgSO4.7H2O, 2 g L-1 glucose, 0.6 mM MgCl2 and 15 ml L-1 olive oil, pH 8.5 at 30 °C for 24 h and low agitation. The amount of lipase produced in the designed medium was in agreement with the predicted values by the statistical method. 16S rRNA cloning and sequencing identified the test organism as Bacillus pumilus.
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