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Articles by B.Z. Guo
Total Records ( 2 ) for B.Z. Guo
  P.M. Dang , B.T. Scully , M.C. Lamb and B.Z. Guo
  In the Southeastern US, peanut plants are often naturally infected with different viruses resulting in economic losses. Limited information is available concerning the identification and characterization of various viruses in peanut. A collaborative effort has resulted in the production of 44,064 expressed sequence tags (ESTs) from developing seeds at three reproductive stages (R5, R6 and R7) of two peanut genotypes, Tifrunner and GT-C20 and from field collected leaf tissues using the same two genotypes. EST sequences were analyzed, assembled and putative functions were determined based on the comparison of these unique peanut sequences with the National Center of Biotechnology Information (NCBI) non-redundant (nr) database using BLASTX program. Sequences matched viral or viral associated proteins were selected for further study. We analyzed the extent of viral sequences in these peanut ESTs and detected the sequences of Peanut mottle virus (PMV), Peanut stripe virus (PStV) and Tomato spotted wilt virus (TSWV). A total of 942 sequences were identified that matched viral sequences in GenBank to PMV (606), PStV (330) and TSWV (6). We also identified peanut plant sequences with homology to pea (Pisum sativum) plant-specific Potyvirus VPg-interacting protein (PVIP), Arabidopsis thaliana tobamovirus multiplication protein 3 (TOM3) and a tobacco TMV helicase domain-binding protein. These host proteins are essential for viral multiplication and movement from cell to cell in host plants. Further study, we examined and identified the presence of these viral and plant sequences in peanut leaves, seeds and roots using RT-PCR. The potential use of these putative peanut virus host responsive proteins may lead to the development of potentially new strategies to control these virus diseases by silencing or disrupting these genes to prevent virus infection and multiplication in host plants.
  X.Q. Liang , M. Luo and B.Z. Guo
  Preharvest aflatoxin contamination in peanut is a serious and world-wide problem concerning food safety and human health. Aflatoxin contamination is known to be influenced by numerous factors. Drought and high temperatures are conducive to Aspergillus flavus infection and aflatoxin contamination. Plant-host resistance is a highly desirable tactic that can be used to manage this problem. Screening, identification and field evaluation of germplasm for resistant traits are important techniques. This review summarizes research progress in the area of host resistance mechanisms to aflatoxin contamination in peanut. Resistance mechanism in peanut to aflatoxin contamination has been studied for nearly 30 years. Some resistance mechanisms and resistant factors have been reported to contribute to the resistance to aflatoxin-producing fungi, but no effective efforts have been made to breed for these traits because the mechanisms to resistance traits might not be still fully understood. Future researches are expected to use advanced biotechnology to understand the comprehensive mechanisms governing the resistance pathways in order to use the information in breeding programs for crop improvement and control of preharvest aflatoxin contamination. The risk of aflatoxin contamination could be prevented before the contaminated grains get into the food chain.
 
 
 
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