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Articles by B.S. Oladele
Total Records ( 2 ) for B.S. Oladele
  Y.D. Dashe , M.A. Raji , P.A. Abdu , B.S. Oladele and M.Y. Sugun
  Antibiotic resistance is often encountered despite multiple antibiotics being used for the treatment of fowl cholera in Jos. This study was conducted to determine the antibiotic resistant profile of Pasteurella multocida isolated from chickens in Jos. A total of 2000 samples consisting of bone marrow, heart, liver, lung and spleen (400 each) were collected from 400 clinically sick chickens between November, 2010 and October, 2011 for the isolation of P. multocida. Swab from each sample was cultured on 7% defibrinated sheep blood, MacConkey and casein sucrose yeast agar. Presumptive colonies of P. multocida were subjected to biochemical characterization. Isolates identified by biochemical tests were further subjected to Microbact GNB 24E test. Disk diffusion method was employed to test the sensitivity of all the twelve P. multocida isolates confirmed by biochemical and Microbact GNB 24E test. The twelve pure isolates of P. multocida were tested for their sensitivity against fifteen different antibiotics. Drug sensitivity test conducted on P. multocida isolates showed that some of the isolates were resistant to penicillin 11 (73%), microlides 9 (60%), sulfanomides 8 (53.3%), cephalosporins 3 (20%) and other new groups of antibiotics 4 (27%). High resistance of P. multocida was recorded for ampicillin (91.7%) followed by amoxicillin/clavulanic acid (83.3%), trimethoprim/sulfamethoxazole (66.7%), erythromycin and anicillin (58.3%) each, while tylosin was (33.3%). This study revealed that there is an emergence of multidrug resistance in some P. multocida strains among chickens in Jos, Nigeria. It is therefore recommended that antibiotic sensitivity test should be incorporated on a routine bases as part of measure to control fowl cholera and minimize the emergence of P. multocida resistance.
  Y.G. Dashe , M.A. Raji , P.A. Abdu , B.S. Oladele and D. Olarinmoye
  Aeromonas species are increasingly incriminated in clinical cases in livestock and humans in Nigeria and the world at large. This investigative study was carried out between November, 2010 and October, 2011 in Jos, Nigeria to determine the isolation rate of Aeromonas species in clinically sick and apparently healthy commercial chickens. A total of 2000 postmortem samples consisting of bone marrow, heart, liver, lung and spleen (400 each) were aseptically collected from 400 clinically sick chickens suspected to be suffering from various clinical conditions and cultured for Aeromonas organisms. Four hundred oro-pharyngeal swabs were also collected from 400 apparently healthy chickens for bacteriological analysis. Swab from each sample was cultured on 7% defibrinated sheep blood and MacConkey. From the bacteriological cultures of the bone marrow, heart and liver of the sick chickens, a total 11 (0.5%) Aeromonas hydrophila isolates were identified by biochemical characterization and Macrobact™ test. Aeromonas organism was not isolated at all from the apparently healthy chickens. The co-occurrence of Aeromonas hydrophila with other pathogens in the sick chickens could have contributed to the observed exacerbation of clinical signs and mortalities in some of the investigated flocks during the study period.
 
 
 
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