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Articles by B.P. Singh
Total Records ( 2 ) for B.P. Singh
  Rupasi Tiwari , H. Dileep Kumar , Triveni Dutt , B.P. Singh , K. Pachaiyappan and K. Dhama
  Today about 7.08 billion people inhabit the earth. Presently to feed every one adequately the world needs 2800 million tonnes of cereals, against which global production is only 2100 million tonnes. This discrepancy in need and production has left over 868 million people undernourished worldwide and 850 million of them live in developing countries. On the other hand rapid urbanization, increasing purchasing power and shift in diet is pushing demand for richer diets and protein of animal origin. By 2050, consumption of meat and dairy products not the butter is projected to increase by 173 and 158%, respectively, as that of 2010. To meet the growing demand and to cope with 9 billion world population by 2050, agricultural production needs to increase by 60% (compared to 2005/2007 production) including of increase in animal production and animal products. Feeding this burgeoning population with the available 0.23 ha of cropland per capita is one of the biggest challenges. Apart from this, the world is facing another biggest challenge of climatic change. Simply, if the “business as usual scenario” continues it requires three earth planets to supply the resources for human population consumption and to assimilate associated wastes it generates in 2050. In the present review, an attempt has been made to throw light on the future challenges of population rise, limited natural resources and climate change to ensure food security in India and the world with major emphasis on sustainable livestock production.
  Aditi Thakur , Suman Sanju , Sundaresha Siddappa , Nidhi Srivastava , Pradeep K. Shukla , Debasis Pattanayak , Sanjeev Sharma and B.P. Singh
  RNAi based “host plant mediated pathogen gene silencing” has emerged as a novel strategy for the efficient control of pathogens infecting various important food crops. Artifical microRNAs (amiRNAs) represent a robust and recently developed miRNA based strategy for the effective posttranscriptional gene silencing in plants. Phytophthora infestans RXLR effector Avr3a suppresses hypersensitive cell death in host cells and responsible for virulence. In the present investigation, the effect of artificial microRNAs are studied on the target transcript, Avr3a gene of P. infestans. Five Avr3a amiRNA gene constructs developed targeting five different regions of Avr3a gene of P. infestans and were transformed into two popular Indian potato cultivars i.e., Kufri Khyati and Kufri Pukharaj. Screening analysis study revealed that most of the transgenic lines were susceptible (15) and few lines (4) were found to be moderately or partially resistance. Target effector gene expression level and the pathogen load were determined to identify whether the resistant observed was RNA mediated. Real time PCR analysis showed that there is reduction in pathogen load as well as in transcript level of Avr3a in resistant lines as compared to the non-transgenic control. This revealed that, the invading P. infestans withdraws the dsRNA/amiRNAs from the host cell leading to the silencing of the Avr3a gene expression causing pathogen death and/or loss of virulence. The amiRNA technology developed in this study appears to be potential and promising for durable and long lasting resistance in potato to combat the notorious oomycete, P. infestans.
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