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Articles by B.O. Oboh
Total Records ( 6 ) for B.O. Oboh
  B.O. Oboh , B. Ogunkanmi and N. Agwu
  This study was conducted to develop a rapid and efficient method for the isolation of genomic DNA from the tropical woody tree, Terminalia catappa L. Fresh young leaves from 35 trees were sampled for the extraction of genomic DNA. The methodology employed excluded the use of liquid nitrogen and an ultracentrifuge with various modifications in the quantities and reagents used. The result of the extraction showed that genomic DNA of good quality and quantity with a spectrophotometric ratio of between 1.7-2.0 for the trees sampled. Result further showed that the extracted DNA on 1% agarose gel had high molecular weight bands following electrophoresis. Thus, we concluded that the modified protocol used for the extraction of genomic DNA in T. catappa which can be easily adapted to other crops produced DNA of good quality and quantity which can be used for PCR based studies.
  L.A. Ogunkanmi , W.O. Okunowo , O.O. Oyelakin , B.O. Oboh , O.O. Adesina , K.O. Adekoya and O.T. Ogundipe
  The genus Corchorus is one of the most important fibre crops, in order to improve this largely neglected crop, it is essential to understand the pattern of diversity in this important crop species. In this study, inter and intra genetic diversity analysis of 40 genotypes from C. olitorius and 40 genotypes from C. incisifolus was carried out using sixteen RAPD markers. The molecular study of the two species classified C. olitorius into two main clusters with five genotypes unresolved while there are three clusters with all the genotypes clearly separated in C. incisifolus. The results of both inter and intra genetic diversities revealed a higher level of allelic diversity in C. incisifolus than in C. olitorius. In the morphological study, higher mean and higher upper range values for all the five quantitative traits in C. incisifolus indicated its superiority over C. olitorius. However, both species show divergent attributes in four qualitative traits but similar in two characters. This could be attributed to higher level of natural outcrossing in C. incisifolus relative to that of C. olitorius. Results of this study therefore show that RAPD marker is a useful tool for assessing inter and intra genetic diversity in the two species of Corchorus. Therefore selection could be made from the diverse genotypes as parents for crosses designed for breeding improved jute cultivars and for producing mapping populations for QTL analysis.
  A.E. Tony-Odigie , K.O. Adekoya , S.C.O. Makinde , B.O. Oboh , L.A. Ogunkanmi and M.A. Fowora
  The genus, Amaranthus, is a typical annual flowering plant valued as vegetables, grains and ornamentals. It exhibits a high degree of interspecies variability and as such enhances biodiversity. Two common grain types (A. caudatus and A. cruentus) and three major weedy types (A. hybridus, A. spinosus and A. viridis) were studied. Phenotypic traits were determined using FAO descriptors for both qualitative and quantitative traits. Extracted DNA samples were amplified through the PCR technique using four RAPD primers (OPA-02, OPA-04, OPB-08 and OPE-01). Statistical analyses for morphological and molecular data were done using the NTSYS Version 2.02j software. Qualitative morphological characters did not significantly discriminate among the five species except for floral morphology. Quantitative characters, however, exhibited wide interspecies variation. Morphological cluster analysis showed that the five species were entirely distinct with a similarity coefficient of 0 except for A. cruentus and A. hybridus which shared an even very low coefficient of 0.093. The RAPD primers generated a total of 150 bands in the size range of 250-2000 bp. Molecular cluster analysis showed that all the species studied shared a similarity coefficient of 0.57 and some individuals within each species were clustered with individuals from other species. For example, most of the randomly selected plants from A. viridis were clustered with plants selected from A. spinosus at a similarity coefficient up to 0.81. This close relationship between A. viridis and A. spinosus may be of medicinal importance for both humans and animals especially since A. viridis is more appealing for consumption. The use of RAPD molecular marker systems in Amaranthus spp. should be advanced so as to impel specific linkage among genes controlling important traits.
  M.O. Sifau , L.A. Ogunkanmi , K.O. Adekoya , B.O. Oboh and O.T. Ogundipe
  The common name “Eggplant” is given to vegetables of several Solanum L. species that are important for human diet and health. The taxonomy and phylogenetic relationships among these taxa are currently unclear due to its large size and tropical center of diversity. This study assessed the genetic diversity in forty nine eggplant and related Solanum species’ genetic resources in Southern Nigeria using one nuclear (ITS) and two chloroplast genes. Analysis of DNA sequence data from the three regions (ITS, trnl C-trnl D and trnl E-trnl F) gave a high level of genetic variability (polymorphism) among the samples studied. A bootstrap value of 100 was observed between S. macrocarpon L. and S. torvum Sw. and between S. macranthum A. Rich. and S. indicum L., closely followed by a value of 99 between S. aethiopicum L. and S. dasyphyllum Schum. and Thonn. All these are indications of a close relationship between these species and a possibility of a common ancestor is strongly proposed. Solanum torvum was often separated out from S. melongena and even where they grouped together they have a low bootstrap value of 3 which is an indication of distant relatedness. The high level of intra and inter specific variations displayed within eggplant accessions and between its relatives as reported in this study could be effectively used in genetic improvement of cultivated eggplant varieties as well as in situ and ex situ conservation.
  K.L. Njoku , M.O. Akinola and B.O. Oboh
  The germination, survival and growth of four accessions each of Glycine max and Lycopersicon esculentum in crude oil polluted soil were investigated in this study. The TGX 1440-1E accession of G. max performed better than the other accessions of G. max while the DT 95/370 accession of L. esculentum performed better than the other accessions of L. esculentum. The accessions of L. esculentum survived more in the crude oil polluted soil than the accessions of G. max but the accessions of G. max grew faster than those of L. esculentum. General analysis of the performances of the accessions of the crops showed that TGX 1440-1E accession of G. max performed better than other accessions of G. max and L. esculentum used in the study. This suggests that intraspecific differences exist in the way plants respond to crude oil pollution. Such intraspecific differences can be utilized in planning for crops to be planted in crude oil polluted soil.
  A.F. Ayorinde , B.O. Oboh , O.A. Otubanjo , A.C. Alimba and P.C. Odeigah
  Although, toxicity of some mosquito repellents has been reported, effects of different doses on some parameters and different sexes is not clear. This study was aimed at determining the toxicity of a commonly used mosquito repellent in Lagos State, Nigeria. The effects studied in rats were based on some hematological parameters, body weight/organ indices (Relative organ weights) and histopathological analysis. The rats were divided into two groups of 6 males and 6 females each and a control of 2 males and 2 females. The two groups were exposed to one and two mosquito repellents per day for one, two and four weeks, respectively. Hematological analysis showed increase in White Blood Cell (WBC) counts in the females and males exposed for 2 weeks and above (p<0.05). Increase was also recorded in the percentage of Mixed Population of White Blood Cells (MXD%) of females and males rats exposed for 2 weeks and above (p<0.05). Platelets (PLT) counts in the female rats (after 2 weeks) also increased when compared with the control (p<0.05). For relative organ weights, significant difference was observed in relative spleen (p<0.05), heart (p>0.05) of both female and male animals and thymus (p<0.05) of the female animals only when compared with control. The histopathological studies showed a severe damage to the lungs whereas no damage was observed in the liver and kidney in both male and female rats. These results show that exposure to Black coil could be detrimental to health.
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