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Articles by B.O. Hayek
Total Records ( 2 ) for B.O. Hayek
  M.A. Ibrahim , N.D. Al-Hmoud , H. Al-Rousan and B.O. Hayek
  Taking into account the impact of monitoring food adulteration on the quality of food products, the aim of this study was to use polymerase chain reaction technology to detect possible adulteration of durum wheat pasta products in the Jordanian market. Cetyltrimethyl ammonium bromide method was applied for extracting genomic DNA from twenty six randomly collected pasta products, the results suggested the suitability of this method for DNA extraction from pasta products. Specific primers were used to ascertain the presence or absence of Dgas44 sequence in the extracted genomic DNA from pasta products, this sequence is known to be present in the genome of common bread wheat and absent in the genome of durum wheat. The obtained results showed that 65.4% of tested pasta products sold in the Jordanian markets are not made solely from durum wheat as indicated by manufacturers.
  N. Al-Hmoud , H. Al-Rousan , B.O. Hayek and M.A. Ibrahim
  The objective of this study was to survey for the genetically modified maize and soybean food products in the Jordanian market. The study was designed to extract genomic DNA of maize and soybean products by cetyltrimethylammonium bromide (CTAB) method and to identify specific genes for maize and soybean, expression control specific genes 35S promoter and NOS terminator by polymerase chain reaction analysis. For confirmation test of the genetically modified maize and soybean food products, nested polymerase chain reaction experiments were performed using internal primers for the detection of the E35S promoter and the hsp70 exon1/intron1 region of maize MON810 and Cp4 EPSPS gene of soybean. Three out of 19 maize food products were identified as carrying amplified DNA fragments of 35S promoter region, the nested PCR test confirmed the presence of MON810 event. One of three soybean food products was identified as carrier DNA fragments of 35S promoter region, Cp4 EPSPS event was not detected.
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