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Articles by B.N. Selvakumar
Total Records ( 5 ) for B.N. Selvakumar
  R. Jasmine , P. Daisy and B.N. Selvakumar
  Methicillin Resistant Staphylococcus Aureus (MRSA) has gained much attention in the last decade, as the MRSA is a major cause of hospital acquired (nosoconical infections). β-lactam antibiotics are the preferred drugs against S. aureus infections, although S. aureus has developed resistance to the β-lactam antibiotics due to the production of chromosomal or plasmid mediated β-lactamases or by producing Pencillin Binding Proteins (PBPs). The Extended Spectrum β-Lactamase (ESBL) producers are highly resistant to several conventional antibiotics. This limits therapeutic options. Hence efforts are now taken to screen few medicinal plants, which are both economic and less toxic, against the ESBL producers. Among the several plants screened, we have chosen to screen the alcohol extracts of a traditional medicinal plant, Elephantopus scaber (Asteraceae) against several clinical strains of ESBL producing MRSA. ESBL producers were screened by double disc synergy test. Methanol, hexane and acetone extracts of Elephantopus scaber were investigated for their ability to inhibit the growth of the chosen ESBL producing multidrug-resistant bacteria by the disc diffusion method. Minimal Inhibitory Concentrations (MICs) were determined by micro broth dilution method. Synergistic interaction of plant extracts with certain antibiotics was also evaluated. On the basis of promising activity, acetone extracts were fractionated and their phytochemical analysis showed the presence of terpenoids, proteins and traces of steroids. TLC bioautography of the fraction showed the active compound to be terpenoids. The strong in vitro antibacterial activity of terpenoid derivatives against ES βL-producing MRSA bacteria suggests the compounds might find wide pharmaceutical use. Further investigations to elucidate the active compound are required.
  B.N. Selvakumar and R. Jasmine
  The aim of this study was to obtain data on susceptibility patterns of bacterial pathogens from patients who attended the CSI Mission General Hospital, in Tiruchirappalli, South India from June 2004-June 2005. During the one-year period of study, the most prevalent etiological agent was Escherichia coli (44.02%), followed by Klebsiella pneumoniae (14.53%) and other species of Enterobacteriaceae (41.45%). These isolates were susceptible to many antimicrobial agents, but those that produced extended spectrum ß-lactaniases (ESBLs) were resistant to most of the antimicrobials. Of all the Enterobacteriaceae tested over a period of 6 months, 236 (25.2%) among 936 were ESBL producers. ESBL production was determined by double disc synergy test. The antibiograms of the ESBL and non ESBL-producers were also compared. Many of the ESBL producers were found to be multidrug-resistant.
  R. Jasmine , P. Daisy and B.N. Selvakumar
  The extended spectrum β-lactamase producers are highly resistant to several conventional antibiotics. Hence efforts are now taken to screen few medicinal plants against the ESBL producers. Among the several plants screened, we have chosen to screen the alcohol extracts of a traditional medicinal plant, Elephantopus scaber (Asteraceae) against ESBL producers. ESBL producers were screened by double disc synergy test. Methanol, hexane and acetone extracts of Elephantopus scaber were investigated for their ability to inhibit the growth of extended spectrum β-lactamases (ESBL) producing multidrug-resistant enteric bacteria by the disc diffusion method. MICs were determined by micro broth dilution method. The crude plant extracts demonstrated zones of inhibition in the range of 5-16 mm against the chosen test bacteria. On the basis of promising activity, acetone extracts were selected to determine their efficacy in terms of Minimal Inhibitory Concentration (MIC), which ranged from 1.6-25 mg mL-1. The acetone extract was subjected to activity-guided fractionation. The most effective fraction had a MIC of 62.5-250 μg mL-1. Phytochemical analysis showed the presence of terpenoids, proteins and traces of steroids. TLC bioautography of the fraction showed the active compound to be terpenoids. The fraction was further tested for their in vivo cytotoxic activity to mammalian system using rats. No marked manifestations were observed. Normal liver and kidney functioning were also observed. The strong in vitro antibacterial activity of terpenoid derivatives against ESβL-producing Gram-negative bacteria suggests the compounds might find wide pharmaceutical use.
  R. Jasmine , P. Daisy and B.N. Selvakumar
  Bioactivity-guided fractionation of the acetone extract of Elephantopus scaber (ES) yielded a new terpenoid, 6-[1-(10,13-dimethyl-4,5,8,9,10,11,12,13,14,15,16,17-dodecahydro-1H-cyclopenta[α]phenanthren-17-yl)ethyl]-3-methyl-3,6-dihydro-2 H-2-pyranone. The structure of the above mentioned compound was elucidated by interpretation of their spectroscopic data. Biological testing of the compound demonstrated significant antibacterial activity against a few multi drug-resistant ESBL-producing clinical isolates. The crude plant extracts demonstrated zones of inhibition in the range of 5-16 mm against the chosen test bacteria. On the basis of promising activity, acetone extracts were selected to determine their efficacy in terms of Minimal Inhibitory Concentration (MIC), which ranged from 1.6-25 mg mL-1. The acetone extract was subjected to activity-guided fractionation. The most effective fraction had a MIC of 62.5-250 μg mL-1. Phytochemical analysis showed the presence of terpenoids, proteins and traces of steroids. TLC bioautography of the fraction showed the active compound to be terpenoids.
  A. Balasundaram , P. Rathna Kumari , George John and B.N. Selvakumar
  The objective of this study was to assess the antimicrobial activity of the methanolic leaf extracts of two Indian medicinal plants Clitoria ternatea and Achyranthes aspera on urinary pathogens. Urinary pathogens were isolated from 127 urine samples taken from subjects diagnosed with Urinary Tract Infection (UTI). Microorganisms were plated on Muller-Hinton agar. Plant extracts were tested by disc diffusion method and the zones of inhibition against pathogenic strains were measured. Clitoria ternatea and Achyranthes aspera showed antimicrobial activity against seven and five strains of urinary pathogens, respectively. Interestingly both plants showed antimicrobial activity against MRSA (Methicilin Resistant Staphylococcus aureus). These results showed that crude plant extracts has wide range of antibacterial activity against UTI pathogen particularly against MRSA.
 
 
 
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