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Articles by B.F. Ogungbe
Total Records ( 2 ) for B.F. Ogungbe
  S.A. Adeola , O.S. Folorunso , O.O. Okedeyi , B.F. Ogungbe , O.B. Babatimehin and O.Z. Thanni
  The emergence of antibiotic resistant bacteria has made the treatments of pathogenic infections difficult. The discovery that some analytes of plant extracts are active against multi-drug resistant bacteria has opened ways to researching into the antimicrobial activity of these phytocomponents. Studies are designed to determine the antimicrobial and antioxidant potentials of the volatile oil of Ocimum gratissimum and its inhibition on partially purified and characterized extracellular protease of Salmonella enteritidis. The oil was extracted by hydrodistillation. Its antimicrobial effect was carried out using microdilution method while antioxidant effect was tested against 1,1-diphenyl-2-picrylhydrazyl (DPPH). The caseinolytic activity of the enzyme was studied with oil as inhibitor. The enzyme was partially purified with dialysis and gel filtration. The oil has scavenging activity of 66.98±1.78% compared to butylated hydroxyltoluene (positive control), 53.29±2.51% (sig. p<0.0001) against DPPH. Salmonella enteritidis was inhibited by this oil with IC50 of 3.98% (v/v). The enzyme had optimal activities at 45°C and pH 7.5. None of the metallic chloride tested produced any significant increase in the activity of the enzyme. Hg2+ and Pb2+ were inhibitors of this enzyme. The oil showed a non-competitive inhibition with Km = 0.33 mg mL-1, Vmax = 1.25x103 μmol min-1 (oil absent) and V’max = 2.50x102 μmol min-1 (oil present). The highest purification fold = 1.88 and the highest percentage yield = 51.74 as compared to the crude enzyme extract. Therefore, the volatile oil of Ocimum gratissimum possessed antimicrobial and antioxidant activities, its inhibition against this protease may be one of the ways of its antimicrobial effect.
  O.S. Folorunso , K.O. Amisu and B.F. Ogungbe
  The ability of microbial cell membrane to bind organic dye is an age long protocol that preludes the identification and characterization of bacteria cells and its components. We employed formation of keratoconjunctivitis, pigmentations and Congo red binding assay to assess the virulence of eight enteric pathogenic bacteria. Staphylococcus aureus and Salmonella typhimurium, at 3.0×108 CFU mL-1, was sufficient to cause keratoconjunctivitis in the eyes of Albino rat (Sereny test), while observing traces in Streptococcus faecalis, Klebsiella pneumoniae, Pseudomonas aeruginosa and Bacillus subtilis. Pigmentation (PCr+) occurred in Staphylococcus aureus, Streptococcus faecalis and Klebsiella pneumoniae. Highest protein secretion was found in Escherichia coli. Hydrophobicity of the microbial cell membrane increased in the order of chemical pre-treatments (0.9% w/v physiological saline, 50 mM Tris HCl, pH 7.8, 50 mM Tris HCl, pH 7.8+50 mM EDTA). Thus, Tris-EDTA adjunct increased hydrophobicity of the membrane to bind more Congo red than other treatments. Virulence strains of Gram-positive bacteria may likely cause a more severe keratoconjunctivitis. Pigmentations favoured Gram-positive than negative probably because of the membrane differentiation. The effect of multiple subculturing on the Congo red binding ability of these bacteria remained inconclusive as less than half of the tested bacteria complied with our hypothesis though, all were expectedly affected, when pre-treated. The virulence of bacteria is pre-determined by the inherent plasmid factors but their expression is a function of the bacteria cells to bind organic dye. Therefore, physico-chemical treatments that promote hydrophobicity of the bacteria cell membrane are likely to reduce their virulence.
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