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Articles by B.C. LI
Total Records ( 3 ) for B.C. LI
  B.C. LI , H. Chen , X.J. Xiao , Wei Han , Qi Xu , Wu Xinsheng , Wenbin Bao and G.H. Chen
  The objective of this study was to evaluate the effect of three cryorotectants (at three concentration each) and two cryopreservation protocols on the preservation of chicken Primordial Germ Cells (PGCs) from gonads at stage 19 and stage 28. The PGCs were cryopreserved using Dimethylsulphoxide (DMSO; 10%, 20%, 30%), glycol (GLY; 10%, 20%, 30%), polyethylene glycol (PEG; 10%, 20%, 30%). In a first series of experiments, we compared viability after three cryorotectants based protocol I and found the viability of PGCs showed very significant percentage (86.53%) (p<0.01) in a freezing media IV. We then compared an cryopreserve protocol I with 5% dimethyl sulfoxide (DMSO)+5% Glycol versus a cryoprotectant protocol II and observed a better viability with the former protocol (85.9% versus 67.4%, p<0.05). Finally, we compared viability of PGCs at three concentration each cryorotectant and found no significant difference p>0.05 between the concentration of 10% and 15% except freezing media III. When the concentration was 20%, the viability of PGCs was the lowest and showed significant difference p<0.05 or very significant difference (p<0.01) compared to other concentration. In conclusion, 5% DMSO+5%GLY with cryopreserve protocol I was the most effective cryopreservation for chicken primordial germ cells.
  X.S. Wu , H. Wu , B.C. Li , G.Y. Zhou , S.Y. Sun , J. Qin , G.H. Chen and H.H. Musa
  To isolate, purify and culture spermotogonia from chicken testicular tissues, a procedure of enzymatic digestion and percoll density centrifugation was adopted for the single cell suspension to obtain purified spermatogonia. The results showed that, using the same purification method, the purity of spermatogonia gained from 6 days old chicken embryo was more than from 13, 15 and 19 days of age; adhesion purification step led to a harvest of 82% of total spermatogonia, which was 15.6% higher than that of direct isolation method; the adhesion time and survival time of spermotogonia before percoll density gradient centrifugation was earlier and longer than after precoll density gradient centrifugation.
  B.C. Li , F.Yu , Q. Xu , L.G. Ni , G.H. Chen , X.M. Cheng , H.H. Musa and T.Z. Liu
  Chicken embryos from stage 15 to stage 45 were studied by means of serial section and light microscopy in order to investigate the relationship between the spermatogonium and the testicular development in early chicken embryos. The results showed that the glycogen in the PGCs (primordial germ cells) cytoplasm reduced gradually at the stage 22-28 (3.5th-5th hatching day). On the stage 29 (6th hatching day), the gonad of the embryo appeared the feature of testis and the glycogen in the PGCs cytoplasm reduced further. On the stage 31 (7th hatching day), the differentiation of ovary or testis was obvious and the glycogen in the PGCs cytoplasm later disappeared. On the stage 34 (8th hatching day), the testicular cord had began to differentiate. The each cord had been solid tubule with the spermatogonium. On the stage 35-37 (9th-11th hatching day), the number of testicular cord had been increased following embryonic development. The spermatogoniums were monolayer located in cord. At this stage, fewer sustentacular cells had been differentiated, while difficult to distinguish. On the stage 38-40 (12th-14th hatching day), the seminiferous tubules had been formed typically, the number of spermatogonium increased as well as sustentacular cells and the interstitial cells in seminiferous distributed in groups. On the stage 40-45 (16th-19th hatching day), the testis on the right was a little bigger than that of the left, the number of spermatogonium increased obviously and appeared like clusters of grape in the middle of seminiferous. Lumina in seminiferous tubule had formed and spermatogonium aligned with different layers.
 
 
 
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