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Articles by B.C. Li
Total Records ( 6 ) for B.C. Li
  H.H. Musa , B.C. Li , G.H. Chen , T.P. Lanyasunya , Q. Xu and W.B. Bao
  Traditional karyotyping is invented in animal research for several decades depend on the analysis of characteristic banding patterns along the length of chromosome. In the present study chicken metaphase chromosomes were obtained by peripheral blood lymphocyte culture techniques, G-band patterns were obtained with trypsin and Giemsa, C-band patterns were treated with barium and the nuclear organizer regions (NORs) were identified by silver staining. All species studied presented a diploid number of 78 chromosomes, with 10 pairs of macro chromosomes including the sex chromosome and 29 pairs of micro chromosomes. G-band patterns were found quite different between breeds. The dark stained of C-band was observed on micro chromosome and W chromosome. Karyotype resemblance near coefficient was possible for breeds clustering. The position of centromers, relative length, arm ratio and the evolutionary distance of chicken breeds was estimated. The application of chromosome karyotype and banding techniques was used to study the origin, evolution and relationship of species, also used for gene location and sex determination. While, in the Medical field was used to identify genetic disease. The techniques was consider as a base for further molecular research, for example FISH.
  B.C. Li , G.H. Chen , J. Qin , X.S. Wu , S.L. Wu and Z.T. Cai
  The aim of this study was to evaluate the efficiency of isolation and culture of PGCs from various tissues of chicken embryos at specific developmental stages including: the circulating blood of stage 14 embryos (hatched for 48-52hrs), the genital ridge of stage 19 embryos (hatched for 68-72hrs) and the gonad of stage 28 embryos (hatched for 128-132hrs). Ficoll density-gradient centrifugation is a standard method for the purification of PGCs from fetal blood. The genital ridge and gonadal tissue contain more PGCs in total but must first be digested with trypsin-EDTA to give a single cell suspension containing a mixture of PGCs and other contaminating cell types. In these experiments, we cultured PGCs from the genital ridge and from gonadal tissue before and after Ficoll density-gradient purification. In all cases, PGCs were subsequently cultured in TCM-199 medium supplemented with 10% fetal calf serum. The results demonstrated that trypsin-EDTA alone of the genital ridge of stage 19 embryos yielded a total of2.7 x 104 per embryo of which 89.5% were viable. After Ficoll density-gradient purification of these cells the yield was 1.8 x 104 of which 87.5% were viable. Processing of the gonadal tissue of stage 28 embryos yielded a total of 3.1 x 104 PGCs per embryo of which 90.0% were viable. It was clear that the PGC yield with trypsin-EDTA alone was higher (P< 0.01) than the yield of the full procedure which included the Ficoll density-gradient purification step. The results of PGC culture from the three developmental stages indicated that the survival time was longest (80-88 hours) for PGCs obtained from stage 19 embryos. At this stage, a large number of PGCs had accumulated in the genital ridge which facilitated the isolation procedure. These results suggest that the highest yield of PGCs per embryo can be achieved by trypsin-EDTA treatment of genital ridge tissue from stage 19 chicken embryos.
  H.H. Musa , G.H. Chen , J.H. Cheng , B.C. Li and D.M. Mekki
  Anka and Rugao chicken breed were reared under the same environment and management. During 12 weeks age the growth rate of Anka breed was found better than Rugao, and similarly within breeds Males were grow faster than females. The results shows that (live weight, carcass weight, dressing out percentage, semi-eviscerated weight, eviscerated weight, breast muscle weight, leg muscle weight, heart weight, liver weight and abdominal fat weight) were significantly (P< 0.01) different in Anka and Rugao breed, however the Abdominal fat % was statistically non significant (P>0.05) between breeds. Males compared to females shows significantly (P>0.01) higher live weight, carcass weight, semi-eviscerated weight, eviscerated weight, breast muscle weight, liver weight and abdominal fat weight within two breeds. However, Leg muscle weight, heart weight were non significantly different (P>0.05). In addition dressing out percentage was significantly (P< 0.05) different between males and females in Anka breed and non significantly in Rugao breed. In Anka chicken breed abdominal fat weight was positively correlated with live weight, carcass weight, breast muscle weight, and percentage of abdominal fat weight; and it was negatively correlated with leg weight. In Rugao breed abdominal fat weight was positively correlated with live weight, carcass weight, breast muscle weight, leg muscle weight and percentage of abdominal fat. However, the percentage of abdominal fat weight was negatively correlated with live weight, carcass weight, breast muscle weight and positively with leg muscle weight and abdominal fat weight.
  H.H. Musa , J.H. Cheng , W.B. Bao , J.T. Shu , B.C. Li and G.H. Chen
  Tissues specimens include (liver, kidney, small intestine and abdominal fat) were excised from lean (Rugao) and fat (Anka) chickens, both breeds were raised under the same conditions. The expression of LDL receptor mRNA in various tissues of genetically fat and lean chickens were determined by semi-quantitative RT-PCR. The level of gene expression was determined as the ratio of integrated peak area for each individual gene PCR product relative to that of the co-amplified ß-actin internal standard. The results shows that the expression of low density lipoprotein receptor mRNA was significantly (p< 0.05) different between abdominal fat and liver tissues, while they were non significantly different between the other tissues. The levels of LDLR1 mRNA expression in intestine, kidney and abdominal fat tissues were none significantly different between genetically fat and lean, while it was significantly (p< 0.05) different in liver. In addition, the levels of LDLR5 mRNA expression in various tissues were non significantly different between genetically fat and lean chickens.
  Musa, H.H , A.H. Suleiman , F.M. El amin , G.H. Chen , D.M. Mekki and B.C. Li
  The West African sheep was originated in the West African countries, brought to Sudan in 1930. These breeds include (Uda, Sankara and Weila) strains. They are normally reared to gather as mixed pastoral flocks, followed different routes during summer and autumn seasons. In the first phase of this study 75 females and 10 males were selected with typical characteristics of the breed, brought to the University of Nyala experimental farm. In the second phase basic information from tribal breeders and their sheep flocks were collected. For each phase flock statistics were recorded and then some reproductive and productive traits were estimated. Season and age effect on mortality of sheep flock was estimated. Likewise feed lot experiment was done to examine the possibility of sheep for improvement of meat production. Body length, heart girth, height at withers and chest depth were measured for lambs at birth to one year age and similar measurements were taken for adult flocks.
  H.H. Musa , G.H. Chen , K.H. Wang , B.C. Li , D.M. Mekki , J.T. Shu and H.P. Ju
  Serum cholesterol, lipoprotein concentration and carcass characteristics data were taken in 30 fat and 40 lean chickens. Breeds were differed significantly (p<0.001) in carcass traits and non significantly (p>0.05) in carcass portions. Color density and pH were also differed significantly (p<0.05), whereas water holding capacity (WHC) and shear force value were non significant (p>0.05). Both breeds showed positive correlation for carcass traits, while abdominal fat was negatively related with breast muscle in lean breed. Significant difference (p<0.05) were observed in cholesterol and LDL and non significant (p>0.05) in triglyceride, VLDL and HDL level. Cholesterol and triglyceride were respectively related with LDL and VLDL. HDL was observed negatively related with triglyceride and cholesterol. Sex was effect significantly (p<0.05) in triglycerides and VLDL level and non significantly (p>0.05) in cholesterol, HDL and LDL level in fat breed. However, lean breed observed non significant difference (p>0.05) for lipoprotein concentrations. Correlation of lipoprotein concentrations and carcass trait were determined. In both breeds lipoprotein concentrations were negatively related with carcass traits. LDL was positively related with breast muscle and abdominal fat weight and triglyceride with live weight in fat breed. In lean breed cholesterol and LDL were presented positive correlation with all carcass traits.
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