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by
B.A. Padder |
Total Records (
2 ) for
B.A. Padder |
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B.A. Padder
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M.D. Shah
,
Mushtaq Ahmad
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T.A. Sofi
,
F.A. Ahanger
and
Aflaq Hamid
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Apple scab, caused by Venturia inaequalis, is one of the most damaging diseases worldwide on apple and is currently managed mainly by scheduled applications of fungicides. The aim of the present study was to understand the pathogen population structure in Jammu and Kashmir which is important for breeding and deployment of resistant cultivars. Twenty-seven isolates of V. inaequalis were sampled from commercial apple growing areas to estimate differences in pathogen populations using Random Amplified Polymorphic DNA (RAPD) 10 -and 20-mer primers. RAPD data analysis grouped 27 isolates in to three major clusters accommodating 10, 10 and 7 isolates each. The categories did not follow any geographic or source cultivar pattern. Allele frequencies among the three populations varied from 0.00 to 1.00. The average genetic diversity within each population (HS) over all loci studied was 0.21, 0.23 and 0.20 in Ganderbal, Pulwama and Srinagar, respectively thereby showing high genetic diversity within each population. The average genetic differentiation at a single locus among all sampled populations (GST) was 0.12. The overall gene flow (Nm) was 3.54 while the gene flow values at a single locus ranged from 0.70 to 69.32. Pair-wise genetic differentiation values (FST) among all loci were low, thereby indicating high diversity among the three populations. Analysis of molecular variance (AMOVA) obtained after clustering the isolates at district level showed highly significant genetic differentiation among populations with 5.46 and 94.54% genetic variability recorded among and within district populations, respectively. Population genetic analysis of V. inaequalis is the first molecular analysis of this pathogen from the India and especially from Jammu and Kashmir, a north-western Himalayan state of India. |
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M.S. Dar
,
Irtefa Mohammed
,
T.A. Sofi
,
F.A. Ahanger
,
M.D. Shah
,
Mushtaq Ahmad
,
Aflaq Hamid
,
A.A. Mir
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Asha Nabi
and
B.A. Padder
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Apple scab caused by a fungus Venturia inaequalis cause enormous losses to growers both qualitatively and quantitatively. In order to manage the crop, farmers routinely spray 8-12 fungicides from pink bud till harvest. Hence, the best strategy to manage the disease is planting apple scab resistant cultivars. In the present study, four races viz., (0), (1), (2) and (1, 2) were reported from various commercial apple growing regions of Kashmir. Apple scab races were spatially distributed in all apple growing regions of Kashmir. Race 1 was most prevalent among the four races. Screening of 31 apple genotypes under controlled conditions revealed majority of commercial cultivars susceptible. Genotypes carrying RVi3 to RVi13 scab resistance genes along with the cultivars American Apirouge, H27, Shireen and Firdous (latter two contain RVi6 scab resistance gene) were found resistant to all the four races present in Kashmir. Resistant genotypes reported in the present study can easily be exploited by breeders for management of apple scab resistance using marker assisted selection approach. |
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