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Articles by B.A. John
Total Records ( 2 ) for B.A. John
  M. Rozihan , B.A. John , C.R. Saad and K.C.A. Jalal
  The effect of partial and complete substitution of fishmeal (FM) for Soy Bean Meal (SBM) on the growth and survival of Macrobrachium rosenbergii were studied. Post larvae (PL 28) with an average individual length (16.20±0.20 mm) and weight (0.032±0.002 g) were stocked in aquarium filled with 5.0 L of water for a period of eight weeks. Six types of diets with different percentage of FM:SBM ratios were formulated and fed to the test animals at the ratio of 0:100, 20:80, 40:60, 60:40, 80:20, 100:0 (diet 1-6, respectively). Significant differences in terms of body weight and orbital length of animal were observed between diets 2, 3, 4, 5 and diets 1 and 6. Larvae fed with diet 3 produced the highest mean weight gain 0.711 g. There was no significant difference in weight gain observed in experiment provided with diet 2, 4 and 5 (~0.4-0.5 g). Larvae fed with diet 1 and 6 showed less weight gain (~0.2 g) with highest percentage of survival (70%) in diet 6 experimental tank. Food Conversion Ratio (FCR), Protein Efficiency Ratio (PER) and Specific Growth Rate (SGR) were also calculated for each experimental diets besides analyzing their proximate composition. It can be concluded that soy bean (diet 3) could be used for the better growth of M. rosenbergii post larvae. It was also understood that complete removal or absolute utilization of fish meal (100%) in diet would directly affect the weight gain in fresh water prawn (p<0.05).
  C.P. Kumar , B.A. John , S.A. Khan , P.S. Lyla and A.S. Kamaleson
  Recent emergence of Chikungunya virus has attracted the researchers to explore its whole genome to predict the structural changes at the genetic level during disease outbreak. Present study was aimed to investigate the mutational patterns at the genome level in Chikungunya virus using advanced software programs. Pair-wise analysis of whole genome (n = 84) of Chikungunya virus revealed that structural genes mutated 2.6 times higher than non-structural genes. The chronological analysis showed that the Chikungunya virus prevalent during 2008 mutated higher than any other outbreaks recorded so far. Number of singleton sites in the variable region of viral genome was found higher when compared to number of parsimonous sites. Chronological and geographical scale analysis of the viral outbreak showed that about 30% of viral genome was subjected to mutation so far. Chikungunya virus isolated from Malaysian geography had underwent more mutations when compared anyother geographical locations. Highest number of singleton mutations were observed in Chikungunya viruses isolated from Malayasian geography whereas highest number of parsimonous informative sites were observed in Chikungunya isolates of India. The mutational analysis also revealed that viral genome contained two times more singleton sites compared to parsimonious sites. About 1207 nucleotides in the structural protein coding region of Chikungunya virus genome was identified as a unique DNA signature witch could be used in precise and fast diagnosing of Chikungunya virus in future.
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