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Articles by B. Yaro
Total Records ( 2 ) for B. Yaro
  Y. Potchoo , I.P. Guissou , M. Lompo , E. Sakie and B. Yaro
  The aim of the present study is to evaluate and compare the antioxidant potential of the leaves extracts of Annona senegalensis (Annonaceae) of Togo versus the one of Burkina Faso. To this end, aqueous methanol and ethyl acetate extracts by splitting and by steeping were achieved and the determination of total polyphenols of which flavonoids was carried out. A survey of the antioxidant activity using the DPPH methods was performed. The content in total polyphenol (3.47 ± 0.03%) and flavonoid (2.33 ± 0.17%) of 70% (v/v) aqueous methanol extract of the specimen from Togo was significantly higher than the one from Burkina (2.66 ± 0.08 and 1.64 ± 0.04%, respectively) (p<0.00001 for total polyphenol; p<0.05 for total flavonoid), whereas, the amount of total flavonoid in the ethyl acetate extract of the species from Burkina (40.38%) was triplicated. For the two types of extracts, the species of Burkina Faso showed an improved antioxidant activity than the one of Togo (IC50 = 8.51 and 21.08 μg mL-1 versus 12.46 and 29.22 μg mL-1, respectively) (p<0.05). These free radicals inhibition activity of the extracts may be due at least to polyphenolic flavonoids identified by means of HPLC assay performed in the preliminary study. These flavonoids were rutin and isoquercetrin as flavanols (specimen from Togo) of which are added epicatechin and catechin derivatives (flavanols) in the specimen from Burkina. The traditional use of plant leaves may imply in part this activity against the free radicals.
  Y. Potchoo , D. Richard , E. Sakie , I.P. Guissou , F. Kini and B. Yaro
  In view to valorize the traditional medicine of Togo, a comparative phytochemical screening was undertaken on the leaves powder extracts of Annona senegalensis of Togo and the one originates from Burkina Faso. The different extracts were obtained by splitting (percolation) and by steeping the vegetable material with appropriate solvents. The main chemical groups of the extracts were characterized by the aid of specific chemical reactions. The 70% aqueous methanol and aqueous extracts were analysed using High-Performance Liquid Chromatography (HPLC). The rate of the relative humidity in Togolese leaves powder was higher (9.7 ± 0.9%) compared to the one from Burkina Faso (7.0 ± 1.1%) (p<0.05). The chemical tests in the limit of their sensitivity and their specificity allowed us to detect some various components hereafter: sterols/triterpenes, carotenoids, flavonoids, anthocyanosides, saponosides and tannins. The HPLC assay has confirmed the presence of some flavonoids, namely rutin and isoquercetrin (specimen from Togo) and the preceding compounds plus epicatechin and catechin derivatives (specimen from Burkina). These differences in the qualitative composition of bioactive compounds of extracts corroborate with the role of the ecosystem on the phytochemical constituents of the two species.
 
 
 
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