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Articles by B. Venkata Raman
Total Records ( 1 ) for B. Venkata Raman
  Malla Sudhakar and B. Venkata Raman
  Background and Objective: Ethnobotanical herbs are an important source for producing treatment cures for various oral infections. There is a huge demand in terms of using medicinal plants against many of the ailments which are both chronic and acute. The aim of our study was to determine the antibacterial activity of the Azadirachta plant tannin fractions especially on the oral bacteria Streptococcus mutans. It studied the tannin fractions on the bacterial growth and its antibiofilm activity along with the down-regulation of the genes responsible for the biofilms. Materials and Methods: Constituent major phytochemicals were screened from the plant extracts and tannin fractions were precipitated and purified. Antibacterial kinetics and antibiofilm assays were performed on the S. mutans by using the purified tannin fractions. The strain was treated with the fractions to screen for the down regulation of the Sortase A (srtA), Antigen I/II (AgI/II) and Competence D (comD) genes and was quantified using real time PCR. A recA, house keeping gene was used as normalize in the study. Results: Chloroform extract was shown to have more antibacterial activity and used for further purification for the tannin fraction. From the antibacterial kinetic study, it was confirmed that 2MIC values were almost similar to the positive control and helped in prolonging the lag phase for exactly 24 h and thereafter with a small log phase. Conclusion: This study on the possible inhibitory role of the tannin fractions of neem extract on the biofilm inducing proteins was confirmed and further its is planned to purify the tannin fractions to study the antibiofilm activity in detail.
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