Asian Science Citation Index is committed to provide an authoritative, trusted and significant information by the coverage of the most important and influential journals to meet the needs of the global scientific community.  
ASCI Database
308-Lasani Town,
Sargodha Road,
Faisalabad, Pakistan
Fax: +92-41-8815544
Contact Via Web
Suggest a Journal
 
Articles by B. Tabaraiee
Total Records ( 1 ) for B. Tabaraiee
  Q. Behzadiyannejad , S.D. Siadat , M. Kheirandish , B. Tabaraiee , H. Ahmadi , D. Norouzian , S. Najar Peerayeh , M. Nejati and M.H. Hedayati
  Production of effective vaccine formulations is dependent on the availability of assays for the measurement of protective immune responses. Antibody- and complement-mediated phagocytosis is the main defense mechanism against Neisseria meningitidis. Therefore, a newly developed phagocytosis assay based on flow cytometry (flow assay) and the Serum Bactericidal Activity (SBA) assay were using sera obtained from rabbit postvaccination with the Outer Membrane Vesicles (OMVs) of Neisseria meningitidis serogroup B was done in order to evaluation of the potential efficacy of (experimental) meningococcal vaccines. The OMVs were injected intramuscularly into of rabbits with boosters on days 14, 28 and 42 after the primary immunization. Phagocytic function of and intracellular oxidative burst generation by rabbit PMN, against Neisseria meningitidis serogroup B, was measured with flow cytometer (Coulter Epics-XL-Profile USA), using dihydrorhodamine-123 as probes, respectively. SBA titers are given as reciprocal Log 2 values of the dilution giving at least 50% killing of the inoculum measured as colony forming units. The results of SBA titers and quantitative flow cytometric analysis of rabbit PMN function in hyperimmun sera with the OMVs revealed a highly significant increase in opsonophagocytic responses and bactericidal antibody against serogroup B meningococci after 56 day (p< 0.05). Both SBA and opsonic activity are crucial for the protection against meningococcal disease. In conclusion, we have shown a very high correlation between opsonic activity and SBA (r = 0.91). Present results indicated that the OMVs could be as a candidate for vaccine toward serogroup B meningococci.
 
 
 
Copyright   |   Desclaimer   |    Privacy Policy   |   Browsers   |   Accessibility