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Articles by B. Singh
Total Records ( 11 ) for B. Singh
  A. Kaur , R. Bala , B. Singh and J. Rehal
  The effect of sodium chloride and different mineral salts on dough rheology for preparation of low sodium bakery products were studied on three commercial flours A, B and C. Five mineral salts viz. KCl, MgCl2, CaCl2, MgSO4 and Na2SO4 were used to replace NaCl at 0, 25, 50 and 100% level. Of all the salts studied for replacement of NaCl, KCl had little effect on farinographs, Na2SO4 had maximum strengthening effect and CaCl2 followed by MgCl2 had distinctive weakening effect. Addition of salts increased the paste viscosities in all the three flours. Paste viscosities were maximum when NaCl was replaced with Na2SO4. From the results it was found that sodium chloride can be easily replaced with other salts without having any adverse effect on dough rheology.
  B.R. Singh , M. Chauhan , R.K. Sindhu , B.R. Gulati , S.K. Khurana , B. Singh , H.S. Yadav and R.P. Yadav
  There are about 20 million equids in India and might be harbouring numerous diseases at any time, however there is little information on prevalence of most of the diseases. A survey of equine practitioners was undertaken to understand the diseases scenario in equids in India. A total of 2000 survey questionnaires were sent to equine practitioners in 64 districts in 14 states, plus 30 private equine farms and four government farms. A total of 532 (26.6%) equine practitioners responded to the questionnaire. On an average each vet treated >1000 animals and >50 equids per year. Out of the 72 health problems of equids identified by the equine practitioners, colic was the commonest followed by trypanosomiasis (surra), lameness, respiratory tract infections including pneumonia and bronchitis and abscess/wounds/injuries group. The information may help in prioritizing future research and resources to improve equine health and welfare.
  N.S. Gill , J. Bajwa , K. Dhiman , P. Sharma , S. Sood , P.D. Sharma , B. Singh and M. Bali
  The objective of present study was to evaluate the therapeutic potential of Cucumis melo seeds traditionally consumed and it was studied for free radical scavenging potential by hydrogen peroxide (H2O2) and DPPH method. Anti-inflammatory activity was evaluated using carrageenan-induced paw edema in rats. Analgesic activity was evaluated by tail immersion and tail flick methods in mice. The percentage scavenging of DPPH radical and H2O2 radical was 74.9% at 300 μg mL-1 and 58.91% at 200 μg mL-1 respectively concentration of. The H2O2 scavenging effect of MECM was 58.91%. The percentage reduction of paw edema was 56.6% at the dose of 300 mg kg-1. The high dose caused significant reduction in pain. Thus it can be concluded from the above results that Cucumis melo seeds have potent anti-inflammatory, antioxidant and analgesic activity.
  B. Singh , D. Katiyar , D.V. Singh , A.K. Kashyap and A.M. Lall
  Rice fields are one of the major biogenic sources of atmospheric methane. Apart from this contribution to the greenhouse effect, rice paddy soil represents a suitable model system to study fundamental aspects of microbial ecology, such as diversity, structure and dynamics of microbial communities as well as structure function relationships between microbial groups. The present study was conducted in rainfed rice fields planted to rice (Oryza sativa) cultivar, NDR-97, to evaluate the variation of population of Methane Oxidizing Bacteria (MOB) in different soil type (Bare, Bulk and rhizosphere) over a period of 13 weeks. Urea was the only fertilizer applied, at a rate of 100 kg N ha-1 in three split doses. The experiment was laid out in a randomized complete block design with three replicate plots for treatments. The soil exhibited higher numbers of MOB in control plots of bulk and rhizospheric (37.4x106 and 58.87x106 cells g-1 dry soil) than in plots treated with urea (28.6x106 and 51.9x106 cells g-1 dry soil) at 80 Days after Sowing (DAS) and were highest in the rhizospheric soil (58.87x106 cells g-1 dry soil) followed by bulk (37.4x106 cells g-1 dry soil) and bare (2.2x106 cells g-1 dry soil) in unfertilized soil but bare (control) soil was attained highest MOB (2.7x106 cells g-1 dry soil) on 40 DAS and MOB significantly decreased in fertilized soil. The concentrations of NH4+-N were significantly (p<0.05) lower in the rhizosphere (1.3 μg g-1 soil) than in bulk (3.7 μg g-1 soil) and bare soils (4.1 μg g-1 soil) on 80 DAS in unfertilized plots. In fertilized soil NH4+-N concentration were increased due to lower number of population at different day’s intervals. The study suggests that the development of the rice rhizosphere brings about a spatial pattern in the distribution of methanotrophic bacteria which increases in size, over time; within the rhizosphere and adjoining bulk soil and that the rhizosphere is a potential microsite of methanotrophic bacterial activity.
  A.R. Kermany , N.M. Mohamed and B. Singh
  Studies on gas sensor technology have intensified with the increasing demand in many fields which require high safety standard. In order to realize this aspect of utilization, gas sensors with improved sensitivity, selectivity and fast response time are critically needed to be developed. With the discovery of carbon nanotubes (CNTs), development is now focused towards CNT-based sensors because of their inherent properties such as small size, high strength, high electrical conductivity, low voltage operation, good stability, long lifetime and large surface area. CNTs are used as the active component in gas sensors. With ionization mechanism, the gas is sensed by measuring its unique electric field breakdown voltage. The non-aligned CNT has been tested as the gas sensing element, but there is still a critical need to produce and test vertically aligned CNTs, since they result in a higher electric field and lower breakdown voltage, producing a more efficient gas sensing device. The objective of the research is to improve the performance of the ionization-based gas sensor using aligned carbon nanotubes array. Testing has been done using different equipments to check the characteristics of the Multi-Walled CNT (MWCNT) sample in comparison with the non-aligned CNTs which was previously designed.
  N.S. Gill , J. Bajwa , P. Sharma , K. Dhiman , S. Sood , P.D. Sharma , B. Singh and M. Bali
  The present study was to investigate the anti-ulcer activity of methanolic extract of Cucumis melo (MECM) seeds. The seeds were extracted with methanol and the extract was evaluated for its free radical scavenging activity by DPPH. It shows maximum antioxidant activity. Thus extract was further evaluated against gastric ulcerations by Pyloric Ligation (PL), Water Immersion Stress (WIS) and NSAID (indomethacin) induced ulcer (NIU) models. In case of PL method, the extract inhibited gastric ulcerations in a dose-related manner (150 and 300 mg kg-1). Then it was evaluated for gastric volume, free acidity, total acidity, ulcerative index. The ulcerative index was also measured in WIS model and NIU with same doses. The MECM caused 57.6, 67.6 and 61.9% inhibition of ulcers in pyloric ligation, water immersion stress, NSAID induced ulcer models respectively at 300 mg kg-1. The results concluded that MECM possess high antiulcer potential which is due to its higher antioxidant activity.
  B. Singh , S. Sharma , G. Rani , A.A. Zaidi , V. Hallan , G.S. Virk and A. Nagpal
  Indian citrus ringspot virus (ICRSV)-free plants of Kinnow mandarin (Citrus nobilis Lourx C. deliciosa Tenora) were produced from a virus-infected plant using nucellar embryo culture. The parent kinnow plant was tested by indirect ELISA and RT-PCR before using its explants. An amplified product of 539 bp (partial cp gene) was obtained by RT-PCR in ICRSV infected plants. The nucellar embryos obtained from seeds collected from immature fruits of the infected plant were cultured on Murashige and Skoog's (MS) basal medium supplemented with various concentrations of 2,4-D and malt extract (ME) alone or in various combinations. Maximum embryogenic callus induction (33.33%) was observed on MS medium supplemented with 2,4-D (9.02 μM) in combination with malt extract (400 mg L-1). Transfer of embryogenic calli to MS medium containing different concentrations of malt extract alone or in combination with ABA resulted in somatic embryogenesis with a maximum of 56.94% cultures in MS medium supplemented with malt extract (500 mg L-1) and ABA (7.56 μM). Cotyledonary shaped embryos when transferred to different strengths of MS medium supplemented with NAA (10.74 μM) developed into complete plantlets in maximum of 72.22% cultures on ½ MS medium. The plantlets were successfully acclimatizated, transferred to screen house and indexed for ICRSV employing indirect ELISA and RT-PCR and all were found negative of virus. A distinct feature of this study is the induction of somatic embryogenesis from nucellar embryos to produce virus-free plants.
  V. Jain , S.K. Verma , S.S. Katewa , S. Anandjiwala and B. Singh
  Silk cotton tree (Bombax ceiba Linn.) is a well known ethnomedicinal plant. Root of this plant was investigated for its antioxidant potential for the first time. Assessment of antioxidant activity was done using 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging assay and Reducing power assay. Preliminary phytochemical screening of the roots showed presence of phenolics, tannins, flavonoids, steroids, saponins and cardiac glycosides. Methanolic extract of the roots showed high amount of phenolics (30.95% w/w) and tannins (15.45% w/w) and a very good DPPH radical scavenging activity (EC50 of 15.07 μg) in a dose dependent manner. The extract showed dose-dependent reduction ability (Fe3+ to Fe2+ transformation) with a maximum absorbance of 1.11 at a concentration of 500 μg of the extract. Acute study in healthy human volunteers showed a significant (p<0.05) rise in total antioxidant status at the end of 4 h after administration of 3 g root powder. This strong in vitro and in vivo antioxidant potential of B. ceiba dry root powder validates its uses in diabetes mellitus and heart disease as described in the traditional medicine.
  N.S. Gill , K. Dhiman , P. Sharma , J. Bajwa , S. Sood , P.D. Sharma , B. Singh and M. Bali
  In the present study free radical scavenging and antiulcer potential of Benincasa hispida seeds was evaluated. The powdered seeds were extracted with methanol. The extract was evaluated for its free radical scavenging activity by DPPH method followed by antiulcer activity using pyloric ligation, water immersion stress and NSAID (indomethacin) induced gastric ulcer model (NIU) in rats. The parameters assessed were gastric volume, free acidity, total acidity, ulcer index and percentage ulcer protection. Ranitidine was used as the reference antiulcer drug. The methanolic extract showed concentration dependent DPPH radical scavenging activity. The methanolic extract of Benincasa hispida seeds (MEBH) inhibited gastric ulceration by decreasing the gastric volume, free and total acidity. The high dose (300 mg kg-1) showed significant reduction in the above parameters which was comparable to the standard drug ranitidine (p<0.05). The MEBH caused 52.7, 67.4 and 61.2% inhibition of ulcers in pyloric ligation, water immersion stress and NSAID induced ulcer models, respectively at 300 mg kg-1. Thus it can be concluded that the seeds of Benincasa hispida possess potent antiulcer activity. The antiulcer action may be exerted through free radical scavenging mechanism.
  Namrata , Lokendra Kumar , D. Ghosh , S.C. Dwivedi and B. Singh
  Nutraceuticals have received considerable interest because of their presumed safety and potential nutritional and therapeutic effects. Pharmaceutical and nutritional companies are aware of the monetary success taking advantage of the more health-seeking consumers and the changing trends resulting in a proliferation of these value-added products aimed at heart health to cancer. Majority of the nutraceuticals are claimed to possess multiple therapeutic benefits though substantial evidence is lacking for the benefits as well as unwanted effects. The present review has been devoted towards better understanding of the nutraceuticals based on their disease specific indications.
  S.V. Singh , A.V. Singh , P.K. Singh , B. Singh , A.S. Ranjendran and N. Swain
  Study aimed to recover and genotype Mycobacterium avium subspecies paratuberculosis isolates from free-ranging wild bison (Bos gourus) found in the Nilgiri hills of Kodai Kanal region of Tamil Nadu (South India). Of the 13 fecal samples screened for the presence of MAP infection, 6 (46.1%) and 8 (61.8%) were positive in microscopic examination and culture, respectively. Further characterization and genotyping of cultures, using IS900 PCR and IS1311 PCR-REA, study first time revealed presence of Indian Bison Type genotype in the bison population of India. More studies may provide link on the evolution of Indian Bison Type genotype which has been widely reported from domestic ruminants in India. Control of MAP infection in bison will be crucial to check transmission to domestic ruminants and conserve fast dwindling population of wild in India.
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