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Articles by B. Sharma
Total Records ( 3 ) for B. Sharma
  M. Kumar , B. Sharma , A. Kumar , H.P. Lal , V. Kumar and M.K. Tripathi
  Toxocara canis is a very important gastrointestinal nematode affecting canines with considerable public health importance. This study was conducted to find out the prevalence and corresponding haemato-biochemical changes in dogs infested with T. canis and to determine its zoonotic implication to dog owners. A total of 121 dogs were screened from October 2008 to May 2009 by direct smear and Mc-master technique, to determine correlation between overall prevalence of T. canis infestation with respect to sex, age, breed, size and season-wise infestation. Haemato-biochemical profile was performed in 24 infested dogs, randomly selected to evaluate changes in Hb, PCV, TEC, TLC, DLC count, serum protein, serum glucose and serum enzymes. The overall prevalence was found to be 28.93%. The prevalence was not influenced by sex but non-descript breeds had significantly higher rates. Pups were more infested than adults and the disease was more prevalent in winters. Dogs having active infection with T. canis infestation showed anemia, leucocytosis and significant eosinophilia (p<0.05). A significant decrease (p<0.05) was observed in serum protein and glucose whereas highly significant increase (p<0.01) was found for both serum enzymes (SGOT and SGPT). Very few dog owners (4.13%) were aware about potential public health significance of the parasite. Survey revealed that unaware owners who belonged to lower/middle/upper middle class, did not maintain hygiene and scheduled deworming and always remain at high zoonotic risk. Being zoonotic, the parasite poses a significant danger to humans mainly children who remain in their vicinity. Thus immediate action needs to be taken to control this parasite and to increase awareness among the dog-owners about the zoonoses being spread by the companion animals.
  D. Sharma , B. Sharma and A.K. Shukla
  Lipases (Triacylglycerol acylhydrolases E.C. are ubiquitous enzymes which catalyze the hydrolysis of triacylglycerols to glycerols and free fatty acids. Lipolytic reactions occur at the lipid-water interface, where lipolytic substrates usually form equilibrium between monomeric, micellar and emulsified states. Due to their high enantioselective and regioselective nature, lipases have been utilized for the resolution of chiral drugs (Flurbiprofen, Naproxen, Ibuprofen and Suprofen) fat modification, fragrance development in dairy product and for the synthesis of personal care product and cosmetics. In addition to these, lipases find use in variety of biotechnological fields such as cheese ripening, detergent, biosurfactant, bioremediation, polymer synthesis, agrochemicals and perfumery and in paper and pulp industry. Lipases are also being used for the development of biosensors for the qualitative determination of triacylglycerols. Nowadays, novel lipases are being developed by site directed mutagenesis and recombinant DNA technology to improve their selectivity and stability.
  R. Acharya , T. Saify , B. Sharma and J. Mehta
  Toxicity is main restrictive factors of plant productivity both in aquatic and terrestrial, natural and anthropically changed environment. Fresh water algae have concerned considerable attention in this respect since they are unpretentious structural organization and short life cycle and can make available indicators for environment monitoring. Thus the study planned to distinguish the toxicity of copper sulphate and sodium chloride on growth and cell plasticity of microalgae species, Scenedesmus that isolated from a polluted site of lower Lake of Bhopal, were accordingly evaluated. At concentration of 1.5 mg L-1 CuSO4 solution, cells become yellow due to the degradation of green pigment whereas NaCl affect only cell size. At concentration 2 to 2.5 mg L-1 of CuSO4, the coenobium cell become scatter with holes and the cell dimension become10.1x2.3 to 9x1.6 μm. At the same concentration of NaCl the cell dimension of Scenedesmus sp. reduced to 11.4x3.4 to 10.9x2.9 μm. The mean dimension changes due to copper toxicity are 10.2x2.5 μm and NaCl toxicity are11.4x3.5 μm. From the above result it is clear that the copper sulphate is highly toxic then NaCl that damage the cell plasticity of Scenedesmus sp.
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