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Articles by B. Ramesh
Total Records ( 2 ) for B. Ramesh
  K.N. Purushotham , H.V. Annegowda , N.K. Sathish , B. Ramesh and S.M. Mansor
  Presence of polyphenolic content in various part of the plant exhibit wide pharmacological activities including antioxidant activity. The present study was designed to evaluate the phenolic contents (total phenols, flavonoid and tannins) and antioxidant properties of ethanolic extracts of flower, leaf, pod, bark and root obtained from Cassia auriculata. Ethanolic extracts of various parts of C. auriculata obtained by sonication extraction techniques are studied for their phenolic contents and DPPH (2,2-diphenyl-1-picrylhydrazine) radical scavenging assay as well as total antioxidant assays using UV visible spectrophotometer. Among the various parts of the plant studied, bark showed significant content of phenolics, flavonoids and tannins followed by the root, leaf, flower and pod. Even bark extract exhibited highest antioxidant capacity in DPPH assay followed by root, leaf, flower and pod with a value of 766.7, 679.3, 644.9, 572.5 and 474.7 mg vitamin C equivalent antioxidant capacity (mg VCEAC)/sample, respectively. In addition, mg VCEAC values obtained from the total antioxidant assay was in the increasing order of bark>root>leaf>flower>pod. Moreover, a strong correlation was also found between phenolic contents and antioxidant values indicating their influence in the found antioxidant activity, hence the bark extract can be employed as an ideal candidate for herbal based pharmaceutical product. Results of the present study also emphasize variation in the chemical composition as well as biological activity ensuring the importance of proper selection of particular part of the plant to evaluate their therapeutic potency.
  P.R. Mohan , B. Ramesh and O.V.S. Reddy
  Rice straw was pretreated biologically with Phenarocheate chrysosporium and subsequently used in the production of cellulases and xylanases employing Asperigillus niger isolate. The FTIR spectrum of pretreated rice straw was compared with standard cellulose library. The spectra of 1048 cm-1 for C-O stretch in cellulose and hemicellulose were observed in rice straw. The enzymatic activity produced by A. niger on biologically Pretreated Rice Straw (PRS) was higher than that of the control treatments, which were carried out with Untreated Rice Straw (URS) and Cellulose (CEL). For medium supplemented with PRS, the peak activities obtained were 1.03±0.06, 1.54±0.08, 0.82±0.14 and 1.02±0.08 U mL-1 for FPase, endoglucanase, β-glucosidases and xylanases, respectively. For the cellulose control, values of 0.68±0.04, 1.24±0.11, 0.54±0.16 and 0.90±0.06 U mL-1 and URS values of 0.48±0.08, 0.94±0.10, 0.46±0.12 and 0.64±0.14 U mL-1 were obtained, respectively. This was substantiated by higher contents of N-acetylglucosamine and fungal protein in PRS samples than the cellulose control and untreated rice straw samples.
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