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Articles by B. Mishra
Total Records ( 2 ) for B. Mishra
  K. Gill , A.K. Singh , S. Kumar , B. Mishra , V. Kapoor , S.N. Das , R.K. Somvanshi and S. Dey
  Ginger has been used in traditional Indian and Chinese medicine and is effective for a wide range of ailments including diarrhea, respiratory disorders, inflammatory diseases, arthritis etc. Recent studies have shown the role of ginger extract in the modulation of biochemical pathways involved in chronic inflammation and have thus provided evidences for its anti-inflammatory property. The aim of the study was to identify and purify a novel protein from ginger rhizomes (Zingiber officinales), of Zingiberaceae family possessing anti-fungal, anti-inflammatory and anti-proliferative properties as elicited using antibiotic diffusion method, surface plasmon resonance, spectrophotometric analysis and flow cytometry, respectively. The purified protein G-24 having molecular mass of 24 kD exhibited a potent anti-fungal activity against the mycelial growth in Fusarium exysporum and Candida albicans. It had shown 60% inhibition of human oral cancer cell line (KB cells) at 10 μM concentration. It inhibited inflammatory enzymes; lipooxygenase (LOX) and cyclooxygenase-2 (COX-2) with KD values of 2.04 μM and 2.74 nM, respectively. This confirmed the anti-inflammatory property of G-24.Thus, concluded that the G-24 protein possessed multiple functions viz. antifungal, anti-inflammation and antiproliferation.
  J. Lin , Z.L. Wu , X.H. Zhang , B. Mishra , J.J. Moore and W.D. Sproul
  Chromium nitride (CrNx) coatings were prepared by reactively sputtering chromium metal target with various nitrogen flow rate percentages (fN2) using a closed field unbalanced magnetron sputtering system operated in dc and middle frequency pulsed condition (100 kHz and 50% duty cycle). In this study, plasma examination proved that a large amount of ions with a wide range of ion energies (up to 65 eV and mainly from 10–30 eV region) was identified in the pulsed plasma compared to the low ion flux and energy (0–10 eV) in a dc discharged plasma. The results showed that the phase structure of CrNx coatings was changed from nitrogen doped Cr(N) to pure β-Cr2N, and to a mixture of β-Cr2N and c-CrN and then to pure c-CrN phases with an increase in the fN2 in both dc and pulsed conditions. However, the pulsed CrNx coatings exhibit lower N concentrations than dc CrNx coatings prepared under the same fN2, which leads to the existing of β-Cr2N phase within a wide range of fN2 (30–50%). In comparison with the typical large columnar structure in the dc sputtered coatings, the pulsed CrNx coatings exhibit dramatic microstructure improvements which benefited from the improved plasma density and ion bombardment from the pulsed plasma, where the super dense and nearly equi-axial structures were observed in a wide range of fN2. The microstructure improvements contributed to the enhancements in the hardness and wear resistance of pulsed CrNx coatings. In the pulsed CrNx coatings, the hardness values were above 30 GPa when the fN2 is in the range of 30–40%, which is related to the formation of the β-Cr2N phase. With the formation of a mixture of β-Cr2N and c-CrN phases in the coatings deposited with 40–50% fN2, a low COF of 0.36 and wear rate of 1.66 × 10− 6 mm3 N− 1 m− 1 can be achieved.
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