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Articles by B. Dhir
Total Records ( 3 ) for B. Dhir
  B. Dhir , S.A. Nasim , S. Samantary and S. Srivastava
  Accumulation of osmolytes in terrestrial plants in response to environmental stresses is well reported and information about aquatic plants is limited. Present study aimed to investigate if the aquatic weed, Salvinia natans accumulates osmolytes/compatible solutes on exposure to various heavy metals. Plants exposed to heavy metals viz. Cd, Cu, Ni, Cr, Mn, Fe, Co, Pb and Zn, were harvested after 48 h and various osmolytes including sucrose, mannitol, proline, glycine betaine and polyamines were estimated using biochemical methods. Results suggested that heavy metal stress does trigger the accumulation of osmolytes such as sucrose, mannitol and glycine betaine. In contrast proline accumulation was not observed. Studies of heavy metal stress on the endogenous levels of polyamines showed presence of free polyamines, while conjugated and bound forms were not detected. Among free polyamines, Putrescine (Put) and Spermidine (Spd) did not show significant decrease in heavy metal exposed Salvinia except Pb and Fe exposed plants. Spermine (Spm) content showed decline in heavy metal exposed Salvinia. The decrease in polyamine levels indicated their possible role in combating oxidative stress induced by heavy metals. Studies suggest that accumulation of osmolytes under heavy metal stress might help in imparting tolerance in Salvinia.
  B. Dhir
  Studies were conducted to assess if the sludge supplementation in soil induces stress in plants. Tomato plants variety Pusa Hybrid 2 was raised in soils supplemented with sludge in different proportions (25, 50, 75 and 100%). Measurement of morphological, physiological and biochemical indices indicated no alteration in the growth till 50%, though an inhibition in growth was noted at higher concentrations. The levels of malondialdehyde (MDA) and hydrogen peroxide (H2O2) did not show any change in comparison to control till 50% concentration. The increase in MDA and H2O2 at higher concentrations (75 and 100%) suggested the induction of oxidative stress. The accumulation of compatible solutes was evident in plants raised in soil supplemented with high proportions of sludge (75 and 100%). Studies suggested that sludge supplementation in soil promote growth in plants up to a certain concentration without inducing stress.
  B. Dhir , S.A. Nasim , S. Nafees and S. Srivastava
  In the present investigation, tomato plants irrigated with treated wastewater were evaluated for their toxicity using short term (acute) animal studies. Male wistar rats fed with tomato plants at the dosage of 150 and 300 mg kg–1 b.wt. for 14 consecutive days showed no symptoms of toxicity. Assessment of haematological parameters such as RBC, WBC, haemoglobin, platelet count showed no significant changes. Plasma and serum analysis also indicated no significant differences in parameters such as urea, AST, ALT, ALP, bilirubin in all the treatments. The present investigations suggested that short term wastewater irrigation in plants do not pose any toxicity to animals. The toxicity depends upon the level of composition of treated wastewater and its transfer to the soil and then plants. Further studies are required to assess toxic effect of long term irrigation with treated wastewater.
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