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Articles by B. Chutichudet
Total Records ( 9 ) for B. Chutichudet
  B. Chutichudet , P. Chutichudet and S. Kaewsit
  An analyzing study to evaluate the relationship between Polyphenol Oxidase (PPO) activity, internal qualities and degree of browning was conducted on lettuce var. Grand Rapids in order to understand the basis changes relating to the appearance of leaf browning and some quality characteristics. For PPO activity, Factorial experiment in completely randomized design was arranged with two factors: developmental stage (Factor A) at four levels (28, 42, 59 and 73 days after planting, DAP) with three different parts of plant (Factor B) (stem, leaf and root), while Completely Randomized Design (CRD) was applied for studying the internal qualities and browning appearance in leaves at 28, 42, 59 and 73 DAP. The experiment was carried out during May-July, 2009, with four replications, ten plants per replication. The results showed that each developmental stage and the various sections of the plant affected PPO activity. The leaf section of the plant particularly affected PPO, especially at 73 DAP. In addition, at the more mature stages of the leaf, more phenolic substance, ascorbic acid content and pH value were apparent, while quinone content decreased at advancing leaf age. In addition, as the plants reached an older stage, the leaf colour in terms of L* and b* decreased. Thus, the appropriate harvest for lettuce var. Grand Rapids should be at 59 DAP in order to face fewer incidence of browning and maintain good quality.
  P. Chutichudet , B. Chutichudet and K. Boontiang
  The siam tulip is a relatively new exported cut flower that has become widely recognized for its attractive colorful pink bract. The main problem limiting this lovely flower’s value is its poor vase life after cutting. The objective of this study was to extend siam tulip’s shelf life by using a substance against ethylene action, 1-Methylcyclopropene (1-MCP). The experiment was arranged in a factorial completely randomized design, composed of two factors: 1-MCP concentration at four levels (0, 300, 600 or 900 ppb) and a period of fumigation at two levels (4 or 8 h) in a hermetically sealed plastic bucket (50 L) at 25°C. The results showed that flowers treated with 300 ppb 1-MCP for 8 h had maximal water uptake, preserved the highest anthocyanin content and the least occurrence of bract browning during postharvest life. No marked differences in weight loss of flowering stalk was observed.
  B. Chutichudet and P. Chutichudet
  A study to evaluate boron, in terms of borax (B4O.2Na.10H2O) or boric (H3BO3) by foliar spraying, on growth and external qualities was conducted on lettuce var. Grand Rapids under field conditions. A Factorial in Completely Randomized Design was arranged with four replications and composed of two factors; two types of boron (borax or boric) with four concentration rates (0, 0.0625, 0.125 or 0.1875%). The results showed that plants-treated with 0.0625% boric had the maximal plant height and bush size. While two types of boron at any concentration had no effect to biomass, chlorophyll content and the leaf colour. Furthermore, plants treated with 0.0625% boric experienced the lowest browning appearance at harvesting stage.
  P. Chutichudet , B. Chutichudet and K. Boontiang
  Patumma is one of the most popular exported cut flowers in Thailand due to its attractive large pink bracts. Its export value, however is limited because of its poor vase life. The objective of this research was to extend patumma’s shelf life by using a substance against ethylene action, 1-Methylcyclopropene (1-MCP). The experiment was arranged in a Factorial in Completely Randomized Design, composed of two factors : 1-MCP concentration at three levels (0, 100 and 300 ppb) with period of fumigation at two levels (12 and 15 h) in a hermetically sealed plastic bucket (50 L) at 20°C. Afterwards, the treated and untreated flower stems were dipped in a plastic bottle filled with distilled water and stored in ambient temperature (27°C, 91% RH). Weight loss of flowering stalk, water uptake by flowering stem, vase life, anthocyanin content and bract colour were recorded every other day at ambient temperature. The results showed that flowers treated with 300 ppb of 1-MCP for 15 h had the least weight loss of the flowering stem and preserved the highest anthocyanin content at 8 DAV. For water uptake by the flowering stem, flowers treated with 100 ppb of 1-MCP for 12 h gave the highest water uptake from six to ten days after vase life (DAV), while the maximal vase life (10.25 days) of flowers treated with 100 ppb of 1-MCP for 12 h was observed. For bract colour, the results showed that treatment with 100 ppb of 1-MCP for 15 h gave the maximal L* and a* values at 12 DAV.
  B. Chutichudet and Prasit Chutichudet
  Pitaya fruit is a popular fruit in Thailand due to its attractive reddish colour and highly delicate flavour. However, the shelf life of this fruit is rather short under ambient condition. The objective of this research was to extend the fresh quality of pitaya fruit by using a natural coating substance, chitosan. The effects of chitosan coating in extending the postharvest life of pitaya fruits relating to weight loss, stomatal conductance, stomatal size in terms of stomatal width and stomatal length, stomatal aperture, wilting percentage and shelf life were investigated. The experiment was arranged in a completely randomized design, composed of coating with chitosan at four levels (0, 1, 2 and 3%) and then stored under ambient temperature. Each treatment consisted of four replicates, ten fruits per replication. The results indicated that fruits treated with 3% of chitosan had the least attributes of stomatal conductance, stomata size in terms of stomatal width, stomatal length and stomatal aperture. In addition, treatment of 3% chitosan also showed the lowest wilting percentage and gave the maximal postharvest life of 8.17 days.
  Chomdao Khumjing , B. Chutichudet , P. Chutichudet and K. Boontiang
  This study was aimed at evaluating the effect of different calcium formulas with various concentration rates on leaf color, contents of phenolic compounds and quinone, Polyphenol oxidase (PPO) activity and browning percentage in Grand Rapids lettuce. A Factorial in Completely Randomized Design was arranged with four replications and composed of two factors: three forms of calcium in terms of calcium chloride (CaCl2), calcium nitrate [(Ca(NO3)2] or calcium oxide (CaO) by soil dressing application with five concentrations (0, 0.5, 1.0, 1.5 or 2.0%). The results showed that plants-treated with 1.5% CaCl2 had the maximal leaf color in term of greenness (a*) values, while treatment of 2% CaCl2 had the lowest contents of phenolic compounds, quinone and activity of Polyphenol oxidase (PPO) which corresponded to the least browning level at harvesting stage.
  B. Chutichudet and P. Chutichudet
  This study aimed to evaluate the effect of two calcium formulas (CaCl2 and CaH4O8P2) at 0.4 and 0.8% on physical and chemical changes to Chiang Mai Pink patumma. The evaluation which included a comparison with control, included an assessment of weight loss, water absorption, bract color, polyphenol oxidase (PPO) activity, phenolic content, browning incidence and vase life. A completely randomized design was arranged with five treatments: CaCl2 0.4%, CaCl2 0.8%, CaH4O8P2 0.4%, CaH4O8P2 0.8% compared with control. The experiment was carried from May to July 2010 with four replications and ten flowers per replication. The results showed that treating with both calcium solutions (CaH4O8P2 0.4%, CaH4O8P2 0.8%) led the flowering stem to absorb less water. At 0.8% CaH4O8P2 caused maximal bract color in terms of a* and b*, highest phenolic content and browning severity. These above characteristics brought about the shortest vase life (7.60 days). The Control flower was found to have minimum PPO activity, phenolic substance and browning damage. Thus, applications of CaCl2 and CaH4O8P2 at 0.4 and 0.8% were found to be not the appropriate substances for maintaining the quality and prolonging the vase life of the patumma’s flower.
  Prasit Chutichudet , B. Chutichudet and S. Kaewsit
  A comprehensive study to evaluate calcium, in terms of gypsum (CaSO4.2H2O) by soil dressing application, on enzymatic browning activity of Polyphenol oxidase (PPO) and internal qualities was tested on lettuce var. Grand Rapids under field conditions. A factorial in completely randomized design was arranged with four replications. The results showed that plants-treated with 50 mg kg-1 gypsum applied at 40 DAP had the maximal fresh weight of 25.83 g plant-1. The internal qualities of the lettuce at harvest showed that plants treated with 50 mg kg-1 gypsum had the maximal chlorophyll content (26.80 mg m-2), while all gypsum concentrations applied in this study, had less content of ascorbic acid than the control plants. Plants-treated with 100 mg kg-1 gypsum affected to the lowest level of PPO activity at week 3 after transplanting. Furthermore, gypsum application had no effect to biomass, leaf colour, the contents of phenolic and quinone in lettuce at harvesting stage.
  P. Chutichudet , B. Chutichudet and K. Boontiang
  The objective of this study was to extend patummas shelf life by using a substance against ethylene action, 1-Methylcyclopropene (1-MCP). The experiment was arranged in a Factorial in completely randomized design, composed of two factors : 1-MCP concentration at four levels (0, 300, 600 or 900 ppb) and period of fumigation at two levels (4 or 8 h) in a hermetically sealed plastic bucket (50 L) at 25°C. Afterwards, the treated and untreated flower stems were dipped in a plastic bottle filled with distilled water and stored in ambient temperature (27.5°C, 91% R.H.). Each treatment consisted of 10 replicates, one flower per replication. The experiment was carried out from June to August, 2008 at the laboratory of the Division of Agricultural Technology, Faculty of Technology, Mahasarakham University, in the Northeast of Thailand. The results showed no obvious differences in shelf life, except that the flower-treated with 600 ppb of 1-MCP for 8 h had the least storage life of 6.40 days. Treatment with 1-MCP had no effect on water uptake, rot appearance and wilting percentage. In addition, flower-treated with 1-MCP at 600 and 900 ppb for 4 and 8 h retained a higher content of anthocyanin by 26.57 and 16.32 mg/100 g Fresh Weight (FW) by 4 and 10 days after vase life (DAV), respectively.
 
 
 
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