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Articles by B. Baradaran
Total Records ( 2 ) for B. Baradaran
  B. Baradaran , J. Majidi , ZM. hassan and J. Abdolalizadeh
  Monoclonal antibodies are key reagents that are used in biomedical researches, diagnosis of immunodeficiency diseases such as IgG subclasses deficiency and treatment of diseases like infections and cancers .For large scale production of monoclonal antibody, hybridoma cells that produce monoclonal antibody against human IgG were injected into the peritoneum of the Balb/c mice which have previously been primed with 0.5 ml Pristane. After ten days, approximately 3 ml ascitic fluid was harvested from the peritoneum of each mouse. Ascitic fluid was assayed for the titer of monoclonal antibody in reaction with human IgG and its cross reactivity in reaction with IgM & IgA. The titer of mAb was 100,000 and didn’t show cross reactivity with IgM & IgA. Immunobloting was done for confirming the ELISA method. In immunobloting, only one sharp band in the heavy chain position of IgG was developed. The subclass of antibody was IgG1 and its light chain was kappa. Ascitic fluid was purified by ion exchange chromatography and the purified monoclonal antibody was conjugated with HRP. The conjugated monoclonal antibody could have application in diagnosis of infectious diseases like Toxoplasmosis, Rubella and IgG class of all other infectious diseases.
  A. Jafari , H. Ejtehadi , N. Taghizadeh and B. Baradaran
  Five specimen of 9 populations and 17 populations of Muscari neglectum were analysed for karyotypic characters and seed proteins analysis respectively using multivariate statistical methods. Karyotypes were asymmetrical and placed in 2A and 2B classes of Stebbins karyotype classification. The level of ploidy were tetraploid, autopentaploid, hexaploid. Some of the popoulations showed decreasing and increasing aneuploidy. Seed protein analysis showed 32 bands which some of them were specific for a population. Cluster analysis of populations on the basis of karyotype and seed protein data carried out and showed close relation of the populations.
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