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Articles by B. Ariyadi
Total Records ( 6 ) for B. Ariyadi
  J. Prastowo , A. Sahara , C. Marganingsih and B. Ariyadi
  In Indonesia, pigeon is one who is familiar with humans, beside can be consumed, usually maintained for hobby or pleasure. One of the parasitic worms that infect the pigeon's kidneys was Paratanaisia bragai (P. bragai). That worm can cause pathological changes in the renal pigeons. Evaluation of renal function can be done through checking of serum creatinine and urea. This study was conducted to identify the renal parasite and its blood urea-creatinine profile on the Indonesian indigenous pigeons. Fifty eight Indonesian indigenous pigeons were identified for renal parasite. Then all of the collected blood was evaluated for urea and creatinine levels. The difference values of urea and creatinine between infected and uninfected renal pigeon were analyzed by t-test. Fifty one percent (30/58) of them were infected by renal parasite. Identification of renal parasite showed the P. bragai. The creatinine levels in infected pigeons (2.650±1.7228) mg/dL were significantly higher than uninfected pigeons (1.732±1.2944) mg/dL. The blood urea levels showed non significant difference between the infected pigeons (7.7700±3.09775) mg/dL and the uninfected pigeons (6.4364±3.60415) mg/dL. The increase of urea and creatinine levels might be not correlated with the number of worms, since the Pearson correlation test showed no significant results. These results will be better if accompanied by histopathology of kidney, uric acid levels and other blood components.
  B. Ariyadi and Sri Harimurti
  Intestinal mucous containing mucins play an essential role as mucosal barrier to prevent invasion in the intestinal tissue of broilers. The aim of the study was to investigate the effect of supplementation of indigenous probiotics lactic acid bacteria on small intestinal histology structure and expression of mucins in the ileum of broiler chicken raised for 35 days. A total of 60 day old chick Lohmann strain broilers were randomly divided into four treatment groups, namely T0, T1, T2 and T3. The T0 group was raised with unsupplemented probiotics, while T1, T2 and T3 were orally supplemented multistrain probiotics at concentration 107, 108, 109 CFU/ml/bird/day, respectively. The results showed that supplementation of indigenous lactic acid bacteria probiotics significantly (P<0.05) increased villus height, villus width of duodenum, jejunum and ileum, as well as increased expression of mucin mRNA in the ileum compared to the control one in broilers. This results suggest that probiotics may stimulate proliferation of intestinal epithelium and regulate mucosal barrier formed by mucin in the intestine of broiler chickens.
  B. Ariyadi , J.H.P. Sidadolog , S. Harimurti , S. Sudaryati and Wihandoyo
  This study was conducted to analyze the relationship between the condition of non-feather distributions and its genetically traits as a multiple allele of the feather condition in the Indonesian indigenous naked neck chickens. Eight males Indonesian naked neck chickens were paired with the five females that had the different condition of non-feathers distribution, namely non-feathers on the neck only (30%); non-feathers on the crop and breast (60%) and non-feathers on the neck, crop, breast and the back of body (90%). Assuming that the non-feathers distribution was caused by a multiple alleles of Na gene and developing of distribution were caused by Na+ as a multiple alleles of Na gene. This study revealed the effect of multiple allele Na+ on the distribution of non-feather areas in the chicken bodies. Analysis of inheritance was conducted by Mendelian heredity with chi-square (X2) analysis. The results, in the Indonesian naked neck chicken, showed that the distribution of non-feathers areas from the neck to the back of body might be caused by genetic of Na+Na+ and Na+Na. The non-feather distribution areas in the crop, breast and the thigh might be genetics effect of Na+na and NaNa (90%). The non-feather distribution areas in the neck or in the crops might be genetics effect of Nana. The normal feathers of chicken might be effect of recessive gene of nana. Moreover, the study showed that the Indonesian naked neck chicken had a lower productivity, higher embryonic mortality and lower hatchability.
  J. Prastowo , A. Sahara , I. Widayati and B. Ariyadi
  This study was conducted to identify the type of Acanthocephala that invaded in the small intestine of laying hens based on morphological examination with Semichon’s Carmine staining. A total of 157 laying hens aged over 50 weeks were used. Worms were collected from the intestine, stored in distilled water at 4°C for 2 days and then stained by Semichon’s Carmine for worm identification. Nineteen laying hens (12%) were infected by worms and 187 Acanthocephala were collected from posterior part of the small intestine. Morphological observation and measurement of worms were the proboscis, trunk, genital system and eggs. The identified Acanthocephala were creamy white without pseudo segmentation, proboscis shaped like a pear with hooks, spines and divided into anterior and posterior parts. The number of hooks on the anterior proboscis were 18-22 rows, each contains 5 hooks, long cylindrical trunk and tapered at both ends. The trunk had no spine with two long Lemniscus and it was not tied. The reproductive system of male worms comprised of testis, cement glands, vesicles gland and bursa of copulatory. The reproductive system of female worms comprised of the uterus and vagina. Eggs were oval shape with 3 layers of walls and contain embryos. Based on morphological examination of Acanthocephala worms, it was concluded that the species was Mediorhynchus gallinarum.
  B. Ariyadi , J.H.P. Sidadolog , S. Harimurti , S. Sudaryati , Wihandoyo and H. Sasongko
  Background: The cecum of the chicken gut may be susceptible to pathogens because it is readily colonized by microbes. The lower segment of the gut is also the primary tissue that permits the invasion of microorganisms from the external environment and the cloaca. Mucins, which are composed of glycoproteins, play significant roles in forming the barrier against infection on the mucosal surface. Objective: The aim of this study was to determine the differences in the mucosal barrier of the lower segment of the gut between Indonesian naked neck chickens and normal feathered chickens. Methodology: The lower segments of the gut (rectum, colon and cecal tonsil) of Indonesian indigenous naked neck chickens and normal chickens were collected. The expression of the mucin 2 gene in the gut mucosa was analyzed by reverse-transcription-polymerase chain reaction (RT-PCR). The localization and molecular sizes of the mucosal glycoproteins were analyzed by Western blot. Wheat Germ Agglutinin (WGA) and jacalin lectins were used for Western blot analysis. Results: The mucin 2 gene was expressed in the mucosal gut of the rectum, colon and cecal tonsil in both naked neck chickens and normal chickens. Western blot analysis showed a single band for both WGA and jacalin from the mucosal gut of the rectum, colon and cecal tonsil in both naked neck chickens and normal chickens. Conclusion: These results suggest that the mucin 2 gene and glycoproteins containing WGA and jacalin positive sugars cover the surface of mucosal gut in both naked neck chickens and normal chickens, most likely to form a mucosa barrier.
  J. Prastowo and B. Ariyadi
  Background and Objective: Chickens infected with Ascaridia galli worms (A. galli ) suffer from slow growth and decreased weight gain. Infection with A. galli usually causes severe damage to the intestines due to migration of the worms in the tissue phase of the intestinal mucosa layer, which affects the proliferation of goblet cells in the duodenums of affected chickens. It is necessary to conduct a study that evaluates the effects of A. galli infestation on the number of goblet cells in the duodenal epithelium of infected chickens. Methodology: In this study, we used a total of 20day-old chicks (DOC). The A. galli worms used in this study were obtained from local chicken slaughterhouses. The worm eggs were embryonized to the L2 stage and inoculated with as many as 5000 eggs/chicken. Samples of the duodenum were taken after the chickens were 6 weeks old, at which time Periodic Acid Schiff (PAS) staining was performed. The elimination rate of the number of worm eggs after the infection was observed every once a week up to week 6 of the research period. Results were analyzed using Student’s t-test and descriptive analysis. Results: There tended to be a higher number of mucin-producing goblet cells in the treatment group than in the control group. The number of worm eggs found increased until the 21st day after infection. The peak of worm egg elimination occurred on the 21st day after infection, with a mean of 2000±250 eggs per gram (EPG); however, the number decreased again on the 28th day after infection. Conclusion: Based on the results of this study, it can be concluded that A. galli infection has a significant effect (p<0.05) on the increased number of goblet cells in the duodenal epithelium of broiler chickens. This worm infection also affects the length of the duodenum and the increase in chicken body weight on a weekly basis.
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