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Articles by B. Prakash
Total Records ( 4 ) for B. Prakash
  Monika Sodhi , M. Mukesh , B. P. Mishra , S. P. S. Ahlawat , B. Prakash and R. C. Sobti
  The present study aims to understand the existing genetic diversity and structure of six native cattle breeds (Rathi, Tharparkar, Nagori, Mewati, Gir, and Kankrej) adapted to the north-western arid and semi-arid region of India based on microsatellite loci. Various diversity estimates, mean number of alleles (12.84); effective number of alleles (5.02); gene diversity (0.769), and observed heterozygosity (0.667) reflected the existence of substantial within-breed diversity in all the investigated cattle breeds. Mean estimates of F-statistics: FIT = 0.144 ± 0.023, FIS = 0.071 ± 0.021, and FST = 0.078 ± 0.014 were significantly different from zero (P < 0.05). The interbreed relationships indicated moderate level of breed differentiation between the six cattle breeds with least differentiation between Kankrej-Mewati pair. The phylogeny structuring further supported close grouping of Kankrej and Mewati breeds. Correspondence analysis plotted Rathi, Tharparkar, and Gir individuals into three separate areas of multivariate space; whereas, Kankrej, Mewati, and Nagori cattle showed low breed specific clustering. This reflected the existence of discrete genetic structure for Tharparkar, Rathi, and Gir, the prominent dairy breeds of the region; whereas, admixture was observed for Kankrej, Mewati, and Nagori individuals.
  B. Prakash , T. Suryanarayana , L. Muniyappa and G. Krishnappa
  Indirect enzyme linked immunosorbent assay was developed to measure specific antibody activity to outer membrane proteins in sera and egg yolk of chickens exposed to Salmonella Gallinarum infection and also following vaccination. The observed end point titers were used to formulate regression equation and the absorbance data obtained at a single serum dilution were converted directly to antibody titer by subtraction method. The assay was carried out at a single serum dilution of 1:320 standardized and the antibody titers of the Salmonella Gallinarum infected flocks were found to be of higher magnitude when compared to vaccinated flocks. The growth-decline trend of the egg yolk antibody levels was found to be similar to that of the serum antibody levels.
  Amitha R. Gomes , L. Muniyappa , G. Krishnappa , V.V.S. Suryanarayana , S. Isloor , B. Prakash and P.G. Hugar
  Fifteen serotypes of E. coli isolated from poultry were subjected to RAPD-PCR using eight oligonucleotide random primers. Amplification with each of the primers resulted in generation of different DNA fingerprinting profile with varied number of bands. Dendrogram based generation of clustering of E. coli serotypes showed two major clusters. The polymorphic amplicons served as markers for E. coli species where as the unique amplicons served as markers for E. coli serotypes. RAPD based fingerprinting provided a rapid means of discriminating E. coli serotypes and considered a valuable tool for molecular typing.
  B. Prakash , G. Krishnappa , L. Muniyappa and B. Santhosh Kumar
  The study was undertaken to elucidate the recent epidemiological status of salmonellosis in India. A total of 23 Salmonella isolates were recovered from different disease outbreaks in different geographical locations. The phenotypic analysis by serotyping, transmission electron microscopy and antibiogram profiles were able to classify the Salmonella isolates based on different serovars. The S. Gallinarum isolates were also classified into different strains based on the resistance pattern to different antimicrobials used. The plasmid profiles of the Salmonella isolates were also found to be serovar specific and the S. Gallinarum isolates were subdivided into different strains based on the geographical origin. There was a positive correlation between the antibiotic resistance pattern and the presence of plasmid within different serovars.
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