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Articles by B. Molina Sanchez
Total Records ( 3 ) for B. Molina Sanchez
  A. Peniche Cardena , D. Martinez Herrera , J.L. Franco Zamora , F. Barradas Pina , B. Molina Sanchez , E.J. Gutierrez Ruiz , J.J. Williams , F. Morales Alvarez and R. Flores Castro
  In this study, the efficacy of vaccination with Brucella abortus RB51 strain as a measure for bovine brucellosis control was evaluated by a clinical assay in double purpose cattle that are naturally infected under tropical conditions. A herd with eight reactors to rivanol test with an initial serum reaction of 5% was selected. Confirmation of infected herd was carried out by isolation and identification of Brucella abortus from reactor animals, using bacteriological procedures. Also, the milk samples were analyzed by PCR technique whereby Brucella abortus infection was corroborated. Vaccinated and non-vaccinated groups were formed with 88 females each. Reactors were not eliminated nor segregated from the population. During 18 months of monitoring three new cases happened in the vaccinated group and therefore the initial serum reaction rate increased from 10 to 12.5%. The rate of vaccinated group remained at 0% due to 100% of protective efficacy that RB51 strain provided to the total vaccinated population (RR = 0; C.I. 95% 0-0). The conclusion is that under extensive double purpose livestock rearing conditions tropical climate, strain RB51 is a biological product efficacious for brucellosis control in infected herds with a prevalence of 6%.
  A. Peniche Cardena , D. Martinez Herrera , J.L. Franco Zamora , F. Barradas Pina , B. Molina Sanchez , E.J. Gutierrez Ruiz , J.J. Williams , F. Morales Alvarez and R. Flores Castro
  Efficacy of vaccination with Brucella abortus S19 vaccine as control measure against bovine brucellosis has been controversial; therefore, it is necessary to know the efficacy of this vaccine under different field conditions. In this study, a clinical assay was performed to establish the efficacy of this vaccine on double purpose cattle. Two groups of one hundred animals each were formed. Infected cattle were not eliminated or segregated. One herd was identified as infected, with four animal reactors to Card Test (CT) and confirmed by Rivanol Test (RT) with a serum reaction rate of 1.2%. Confirmation of infected herd was carried out by isolation and identification of Brucella abortus colonies and PCR on milk samples from RT reactor animals. In 18 months, the number of infected animals increased to eight females, seven within the non-vaccinated group and one in the vaccinated group for a serum reaction rate in the non-vaccinated group of 5.8% and the vaccinated one of 0.8%. Thus, in this period the accumulated serum reaction rate for both groups was increased from 1.2 to 3%. Vaccination efficacy of strain S19 was 86% and the risk of getting the disease in these animals was very low (RR = 0.112; I.C.95% 0.014-0.887). It is concluded that strain S19 vaccine is efficacious in the control of brucellosis in herds with a 3% prevalence of the disease; yet, before its use, interference of diagnosis problems that are produced should be assessed to properly evaluate economics and vaccination efficiency.
  D.I. Martinez Herrera , J.A. Morales Morales , A.E. Peniche Cardena , B. Molina Sanchez , M.A. Rodriguez Chessani , R. Loeza Limon , M.L. Robledo Salinas , J.F. Morales Alvarez and R. Flores-Castro
  With the aim to evaluate strain RB51 Brucella abortus vaccine under field conditions in goats and ovine communities, located at Perote and Coffer Region in Veracruz, Mexico, where, prevalence rates varies, several vaccination operatives were done between 12 to 15 months, using 3x108 to 3x109 Colony Forming Units (CFU) of such vaccine for each animal. A total of 5,168 goats or ovine females 3 months old and older, were vaccinated. Those animals belonged to 322 herds in different communities. Vaccination was done despite their reproductive condition. Twenty animals were randomly selected in each herd and blood samples collected, before and after 12 to 15 months of vaccination, in order to determine brucellosis seroprevalence. Sample size for each community was estimated by a probabilistic model, with unknown population and 50% known prevalence rate (n = 1-p/pv); where, n corresponds to sample size, p for prevalence rate and v variation coefficient (0.05), blood samples were taken by jugular vein punction using vacuum tubes system. The sera collected were tested by using 3% antigen concentration card test as screening and complement fixation as confirmation test, according with Mexican regulations. During the first sampling tests it was found that seroprevalence rates in the communities were: 0, 0.5, 4.5, 5, 38 and 1.4%. The rates of seroprevalence in the second sampling were: 0, 0, 5.5, 0 and 0%. The rates of serum reactors were reduced 80% from different rates to 0.0%. According with these results, RB51 Brucella abortus strain vaccination provided a successful response at the indicated dosage. It is conclusive that RB51 Brucella abortus strain goat and ovine vaccination at communities from Perote and Coffer Region is useful for brucellosis control.
 
 
 
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