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Articles by B. Anand Kumar
Total Records ( 2 ) for B. Anand Kumar
  K.V. Deepika , B. Anand Kumar , S. Gnanender and P.V. Bramhachari
  Biosurfactant-producing bacteria, isolate KVD1, was isolated from Krishna river delta mangrove sediments. The taxonomic identification of KVD1 strain was done by sequencing of 16S rRNA gene showed that it formed a coherent cluster with the clad that comprised of Pseudomonas aeruginosa to be its closest phylogenetic neighbour. Strikingly, for the first time Pseudomonas aeruginosa strain KVD1 isolated from mangroves was shown to produce highest biosurfactant (4.08 g L-1) in optimised MSM containing 1.5% diesel as sole carbon source, 1% (NH4)2SO4 as nitrogen source and 1 g L-1 NaCl, at pH (7±2), 30°C, 150 rpm. The biosurfactant was obtained by cold acetone extraction method showed emulsification activity of E24% = 61±2% with n-hexadecane. The stability of the biosurfactant at different salinity, pH and temperature was also investigated. This biosurfactant also exhibited significant efficiency in oil recovery sand experiment. The present study emphasizes that biosurfactant produced by strain KVD1 is critical to open new biotechnological applications in enhanced oil removal from spillages and also as cleaning and emulsifying agents in the industry.
  P.V. Bramhachari , B. Anand Kumar , K.V. Deepika and S. Gnanender
  Microorganisms play a pivotal role in biogeochemical transformations and hence termed as natural decontamination agents. Therefore, screening for tributyltin (TBT) resistant and degrading bacteria is relevant for selection of isolates with decontamination ability of TBT polluted areas. With this rationale, 105 strains were isolated from sediment and surface waters of Vishakapatnam shipping harbor and their tolerance to TBT was evaluated. Further screening was done based on the ability of bacteria to grow in Minerals Medium (MM) containing 3 mM TBT as sole source of carbon. Ten selective TBT resistant isolates showed cross tolerance to six heavy metals and ten antibiotics. Among them, one interesting isolate VBAK101 showing highest TBT resistance (4 mM) and maximum growth yield (A600 nm of 1.6 after 36 h) was selected for further study. The taxonomic identification of strain VBAK101 strain was done by sequencing of 16S rRNA gene. The maximum-likelihood algorithm showed that it formed a coherent cluster with the clad that comprised of Alcaligenes sp., to be its closest phylogenetic neighbor. Under TBTCl stressed condition, VBAK101 produced more exopolysaccharide (EPS) 92 μg mL-1) compared to control conditions (82 μg mL-1). Overall, synthesis of EPS in bacteria is a protective barrier against TBTCl stress. These results suggest that Alcaligenes sp. VBAK101 is potentially useful for the bioremediation of TBT contamination.
 
 
 
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