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Articles by Azra Quraishi
Total Records ( 7 ) for Azra Quraishi
  Muhammad Shah Zaman , Azra Quraishi , Ghulam Hassan , Raziuddin , Sardar Ali , Abdul Khabir and Naheed Gul
  The effect of three different auxins viz, naphthalene acetic acid (NAA), indole acetic acid (IAA) and indole butyric acid (IBA) each at four levels (0, 0.1, 0.5 and 1.0 mg l-1) was evaluated on meristem culture of Potato (Solanum tuberosum L.) for production of virus-free plantlets. Maximum plantlet height (8.3 cm), largest number of nodes/plantlet (7.3) and highest number of leaves/plantlet (8.9) were recorded at 0.5 mg l-1 of NAA followed by IBA at 1 mg l-1, whereas extensive number of roots/plantlet (23.7) as well as the earliest microtuber formation (17 days, after transplantation) were recorded at 1 mg l-1 of IBA followed by NAA at 0.1 mg l-1. Largest root (4.2 cm) was observed for IAA at 1 mg l-1 concentration. Enzyme Linked Immunosorbent Assay (ELISA) test proved that all plants were free of Potato Virus X (PVX), Potato Virus Y (PVY) and Potato Leaf Role Virus (PLRV), except one which was positive for Potato Virus X (PVX) virus.
  Zubeda Chaudary , Imran Feroz , Waseem Ahmed , Hamid Rashid , Bushra Mirza and Azra Quraishi
  The varietal response of Feston and Nagina was tested using hypocotyl and leaf disc as explant source. The callus formation was achieved on MS medium supplemented with 2,4-Dichlorophenoxy acetic acid (2,4-D) and on MS medium supplemented with Napthalene Acetic Acid (NAA) and Benzylamino Purine (BAP) from both explant sources. Shoot formation from calli of Feston and Nagina were successfully obtained on different hormonal levels of BAP & NAA on MS medium. Maximums shoot percentages of calli derived from hypocotyl (56.00%) and leaf disc (42.30%) was achieved on MS medium with 0.05mg/l IAA and 4.0mg/l BAP for Feston. For Nagina, maximum shoot percentage was obtained from leaf explant (39.13%) and hypocotyl (32.00%) on similar medium. Shoot regeneration produced 3.137 and 2.353 mean number of shoots/explants for hypocotyl and leaf disc of Feston whereas Nagina responded with 2.187 and 1.783 mean number of shoots/explant for leaf disc and hypocotyl respectively.
  Hamid Rashid , Syed Yassir Abbas Bokhari and Azra Quraishi
  A protocol has been developed for callus induction, regeneration and hygromycin selection of rice (Oryza sativa L. cv. Super Basmati). Mature seeds of rice were used as starting material for callus induction and cultured on Murashige & Skoog medium (MS) and N6 medium having different levels and combinations of 2,4-Dicholro Phenoxy Acetic Acid (2,4-D). 2,4-D @ 2 mg/l gave good response to callus induction. For regeneration, calli were cultured on MS medium having different concentrations of Naphthalene Acetic Acid (NAA) and Benzyl Amino Purine (BAP). Medium containing NAA @ 1mg/l with BAP 5 mg/l showed best results with regeneration frequency of 45.3%. Hygromycin at the concentration of 50 mg/l proved to be lethal for secutellum-darived calli of Super Basmati.
  Farheen Niaz and Azra Quraishi
  Somatic embryogenesis was studied in sugarcane cultivars CPF-237 and SPF-213. Explants used were leaves, lateral buds and pith. MS medium along with NAA and 2,4-D in various concentrations were used and it was observed that 1.0 mg NAA and 3-mg/l 2,4-D was optimal for embryogenesis. Leaf portion showed maximum embryogenesis and proved a better explant source than pith.
  Muhammad Ramzan Khan , Hamid Rashid and Azra Quraishi
  Effects of different growth regulators on uni-nodal explants of field-grown olive (Olea europaea L.) cv. Leccino were investigated. Single-node cuttings were obtained from 8th bud pair. Disinfection protocols were standardized by using 0.1% mercuric chloride for five min followed by 50% Clorox for 15 min. sterilized cuttings were tested on MS medium supplemented with various plant growth regulators viz. gibberellic acid kinetin benzylaminopurine, and isopentenyladenine. These growth regulators were used singly in different combinations ranging from 1.0 to 8.0 μM l -1 In vitro. GA3 @ 4 μM l -1 was found to be more effective in enhancing shoot elongation. A higher concentration of kinetin, BAP and 2iP induced more callusing and hyperhydrated microshoots. Inclusion of polyvinylpyrrolidone @ 0.2 g/l in media was found to be successful to eliminate phenolic exudates.
  Aish Muhammed , Mustafa Sajid , Iqbal Hussain and Azra Quraishi
  The objective of this study was to observe the in vitro morphogenetic response of a commercial hubrid SF-187 which is used in sunflower breeding programme. Basal MS media supplemented with NAA and BA was used. Cultures were incubated in complete obscurity at a temperature of 25°C. Morphogenesis and callogenesis were observed after 14 days of incubation. When exposed to light, shootlets turned green within two days. A maximum of fifty plants were regenerated from single explant when cultured on MS medium supplemented with 2.0 mg/l each of BAP and NAA. The plantlets were rooted when subcultured on MS media supplemented with 0.5 mg/l IAA.
  Muhammad Ramzan Khan , Hamid Rashid and Azra Quraishi
  Hypocotyl explants from in vitro seedlings were cultured aseptically on Gamborg (B5) medium supplemented with various concentrations and combinations of dichlorophenoxyacetic acid, indoleacetic acid, naphthalene acetic acid and benzylaminopurine for callus formation and shoot regeneration. 2,4-D alone @ 2 mg/l gave the highest callus formation (96%). The results revealed that BAP @ 2 mg/l with IAA 0.5 mg/l was the most appropriate combination to produce the multiple shoots (14.5 shoots per culture). Efficient rooting was achieved on half-strength MS medium supplemented with 0.250mg/I IBA and 0.125mg/l IAA. In vitro raised plantlets were transferred to potted soil and finally to experimental fields to assess their acclimatizing potential.
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