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Articles by Azamal Husen
Total Records ( 4 ) for Azamal Husen
  Azamal Husen
  Rooting and sprouting traits of single-node leafy cuttings (SNCs) of Grewia optiva have been studied in relation to age of donor plants and auxin treatments. SNCs from 1-, 5-, 10- and 15-year- old donors and treated IBA 3000 mg L-1 gave the best responses. Periodic sampling was performed to examine Total Soluble Sugar (TTS), Indole Acetic Acid (IAA) oxidase and peroxidase (PER) activities in the rooting zone during Adventitious Root Formation (ARF). TTS decreased with passage of time from 0 to 40 days of analysis. Auxin treatments exhibit higher TSS in all donors. IAA-oxidase and PER reduced with maturation. IAA-oxidase was highest in untreated cuttings while auxin treatments had enhanced PER activity. IAA-oxidase was found to decrease up to 20 days and increased thereafter. PER increased with time from the day of planting upto its 30 days, followed by a remarkable decline at 40, irrespective of age of donor plants or auxin treatments. Both enzyme activities remained higher in SNCs of 1-year-old donors which rooted more profusely as compared other donors. Present study suggests auxin treatments have activated carbohydrate metabolism to release energy for ARF. IAA-oxidase and PER activities seems to involve for triggering, initiation and elongation of root primordia.
  Azamal Husen and Sayyada Khatoon
  Adventitious root formation in woody plants is highly influenced by the process of physiological ageing. There are increasing efforts to identify markers for distinguishing between mature and juvenile stages of trees. However, it appears to be very little information on this aspect at the chemical and molecular levels. The aim of the work was to identify potential chemical marker of juvenile and mature state; and accomplish maximum rooting in teak (Tectona grandis Linn. f.) leafy shoot cuttings. Experiments with teak leafy shoot cuttings of three different physiological ages 30- and 15-year-old (mature); and 2-month-old (juvenile) were conducted in mist propagation system. Maturity of donor teak plants decreased rooting ability. Cuttings obtained from 2-month-old donors rooted more profusely in comparison to 15- and 30-year-old donors. Indole-3-butyric acid treatment at 4000 mg L-1 significantly increased rooting percentage and produced highest number of roots. High Performance Thin Layer Chromatography (HPTLC) analysis of anthraquinones (AQs; C14H8O2) was utilized to detect qualitative and quantitative differences in AQs in stem tissues from 2-month, 15- and 30-year-old donor plants. The HPTLC analysis showed that AQs varied from 2.8 to 18.3% in cuttings derived from 2-month-old donor plants, while the variations were 4.9 to 27.3% and 11.8 to 43.4% in those from 15- and 30-year-old donor plants, respectively. Altogether, data support that AQs could be a reliable marker for maturity vis-à-vis juvenility in teak.
  Zelalem Getnet , Azamal Husen , Masresha Fetene and Gietahun Yemata
  Drought is a major limiting factor for crop expansion. Currently efforts are focused on improving crop genotypes for drought-prone areas. Considering this, a field experiment was conducted in the Shewa robit (a drought-prone area), Amhara Regional State, Ethiopia to evaluate the various growth attributes, physiological, biochemical and agronomic performances of different Stay-Green (SG) Sorghum accessions. Three Sorghum collections (Sorcoll) namely, Sorcoll 141/07, Sorcoll 146/07, Sorcoll 163/07 and a check (Afeso) genotypes were used. The experiment was arranged in a randomized complete block design. Data on the height, girth diameter, number of tillers, number of green leaf, leaf senescence, leaf area, chlorophyll content index, flowering, physiological maturity, Relative Water Content (RWC), net photosynthetic rate (Pn), transpiration rate (E), Water Use Efficiency (WUE), maximum quantum yield of PSII efficiency (Fv/Fm), leaf pigments, proline, nitrogen, protein, soluble sugar contents, Shoot Dry Weight (SDW), Root Dry Weight (RDW), Root to Shoot Ratio (RSR) and yield revealed significant difference among the accessions at p<0.05 level. Afeso and Sorcoll 163/07 showed better stress tolerance and the SG property. These accessions were also given maximum grain yield per hectare, while Sorcoll 146/07 has shown early maturity. Further, the correlation analysis revealed that girth diameter, number of tillers, chlorophyll content, leaf area, RWC, Pn, E, WUE, Fv/Fm, rate of leaf senescence, SDW, RDW and RSR had significant and positive relation with grain yield per hectare; and have been found to be effective screening tools for stress tolerance and SG property. Thus, they can be used in Sorghum breeding program.
  Alachew Embiale , Muhammad Hussein , Azamal Husen , Samuel Sahile and Kasim Mohammed
  In recent years, drought has been a serious problem in Ethiopia and elsewhere which has adversely affected plant productivity. This study aimed to investigate the effects of water stress on growth, biomass and foliar characteristics in three cultivars (Brukitu, Tegegnech and Adi) of Pisum sativum. The control plant pots were uniformly irrigated at 3 day intervals to maintain 100% field capacity. Water-stress conditions were imposed by subjecting plants to a gradual decrease of soil water availability such as watering at 6 day intervals (slight-stress condition), 9 day intervals (mild-stress condition) and 12 day intervals (severe-stress condition). Results revealed significant differences among the cultivars, water-stress treatments and their interaction, indicating the cultivars variability and differential response to water stress. Water stress adversely affected growth, biomass production, leaf water status and other leaf characteristics such as pigment concentration (chlorophyll a, b and total chlorophyll), maximum quantum yield of photosystem II (PS II) (Fv/Fm), net photosynthetic rate, stomatal conductance and transpiration rate in all cultivars, as stress level was increased in comparison to control plants. The relatively less decline in the studied parameters of Tegegnech exhibited a reasonable tolerance ability of this cultivar, whereas Brukitu and Adi proved to be sensitive to water-deficit condition.
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