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Articles by Azadeh Mohammadirad
Total Records ( 9 ) for Azadeh Mohammadirad
  Mojdeh Mohammadi , Shokoufeh Atashpour , Nazila Pourkhalili , Amir Nili-Ahmadabadi , Maryam Baeeri , Azadeh Mohammadirad , Shokoufeh Hassani , Shekoufeh Nikfar and Mohammad Abdollahi
  Increased oxidative stress plays a role in the pathogenesis of cellular death and β-cell failure. The aim of this study was to evaluate the action of different types of phosphodiesterase (PDE) inhibitors including milrinone (PDE-3), rolipram (PDE-4) and sildenafil (PDE-5) on viability, production of Reactive Oxygen Species (ROS) and secretion of insulin from isolated rat pancreatic islets. Pancreatic islets were carefully isolated and incubated in RPMI 1640 for 24 h. After overnight incubation, islets were picked up and divided into ten in each groups. Then, milrinone, rolipram and sildenafil at doses of 0.1, 1, 10 and 100 FM were added to islet groups and incubated for further 24 h. Then static insulin secretion at 2.8 and 16.7 mM concentrations of glucose, was tested. Then the viability of cells, level of ROS and insulin were examined. The results of static experiments showed that secretion of insulin increased significantly in response to glucose at both basic (2.8 mM) and stimulation (16.7 mM) levels by the lower doses of tested PDE inhibitors. The level of ROS at the lower doses of milrinone decreased. The viability of islets at the lower doses of all of PDE inhibitors were increased; however, viability at the higher doses of sildenafil and rolipram reduced significantly. Milrinone was the most effective PDE inhibitors on the function of isolated pancreatic islets. PDE inhibitors show the most significant anti-oxidative effects at lower doses. Concerning improvement of isolated islets function, PDE-3 inhibitor is the best among tested compounds. PDE inhibitors may help management of diabetes and facilitate conditions of islet transplantation.
  Azadeh Mohammadirad , H.R. Khorram-Khorshid , Farhad Gharibdoost and Mohammad Abdollahi
  This review focuses on the efficacy of Setarud (IMOD) that is an effective new drug in the management of different conditions in animal and human. IMOD is a new natural mixture that demonstrated immune modulating activity in preliminary investigations. To conduct this review, all relevant databases were searched up to 20th August 2011 for the term IMOD or Setarud and all human, animal and in vitro studies considered the effects of IMOD. A total of 13 studies were included. Some human studies showed anti-inflammatory and anti-oxidative stress effects of IMOD by lowering tumor necrosis factor alpha (TNF-α) and CD4 (a glycoprotein expressed on the surface of T helper cells, monocytes, macrophages and dendritic cells) that are involved in process of inflammation and oxidative stress consequences. The positive effects of IMOD in the control of experimental models of colitis, diabetes, hyperchlosteromia, polycyctic ovary syndrome and liver injury were found that were mediated mainly through antioxidant and anti-inflammatory properties. IMOD has also enhanced secretion of insulin from the rat isolated pancreatic islets and human B lymphocyte. It is concluded that IMOD has optimistic effects on oxidative stress and pro-inflammatory conditions in various diseases which most probably is resulted from combinational effects of herbs and the elements present in the mixture of IMOD.
  Seyedeh Farnaz Ghasemi-Niri , Sara Solki , Tina Didari , Shilan Mozaffari , Maryam Baeeri , Mohammad Amin Rezvanfar , Azadeh Mohammadirad , Hossein Jamalifar and Mohammad Abdollahi
  Alteration of intestinal microflora has an important role in Inflammatory Bowel Disease (IBD) and probiotics by balancing microflora and modulating inflammatory cytokines seem effective in management of IBD. In the present study, two combinations of probiotics Lactobacillus casei (L. casei) plus Bifidobacterium bifidum (B. bifidum) or plus Saccharomyces boulardii (S. boulardii) were tested for their potential to prevent or treat experimental colitis in rats. Nine groups of animals including sham (normal group), control (vehicle-treated), dexamethasone as standard, treatment groups (oral administration of L. casei and mixture of (L. casei-B. bifidum and L. casei-S. boulardii after induction of colitis) and prevention groups (oral administrations of L. casei and mixture of (L. casei-B. bifidum and L. casei-S. boulardii before induction of colitis) were used. Tumor necrosis factor-α (TNF-α), myeloperoxidase (MPO) and lipid peroxidation (LPO) were determined in all groups. In treatment groups, histological scores and TNF-α, MPO and LPO levels attenuated significantly but colonic lesions in the prevention groups did not recover. The results revealed that, combination of L. casei-B. bifidum and L. casei-S. boulardii alleviated inflammatory parameters in colitis but these combinations were ineffective to prevent colitis. In addition, we found that mixture of probiotics is significantly more effective than L. casei group in improving barrier function of epithelium in experimental colitis model.
  Mahnaz Khanavi , Marzieh Taheri , Afsaneh Rajabi , Saeed Fallah-Bonekohal , Maryam Baeeri , Azadeh Mohammadirad , Gholamreza Amin and Mohammad Abdollahi
  The aqueous, dichloromethane, ethyl acetate and methanol extracts of dried aerial fruiting parts of Centaurea bruguierana ssp. belangerana were investigated for hypoglycemic mechanism in diabetic rats. Diabetes was induced by intravenous administration of streptozotocin-alloxan. The methanol and ethyl acetate extracts were administered in a single effective dose of 200 mg kg-1 and dichloromethane and aqueous extracts were administered in a single effective dose of 400 mg kg-1. Blood glucose was determined every 1 h until 3 h post administration of the extracts. In the second experiment, the liver was surgically removed 3 h post treatment of diabetic rats with various extracts, homogenized and used for measurement of key enzymes of glycogenolysis (glycogen phosphorylase, GP) and gluconeogenesis (phosphoenolpyruvate carboxykinase, PEPCK). Treatment by dichloromethane, ethyl acetate, methanol and aqueous extracts and the glibenclamide, reduced blood glucose to 41.7, 55.0, 45.7, 29.5 and 34.5%, respectively. The aqueous extract showed the best effect in reduction of hepatic PEPCK activity (84.0%) and increased hepatic GP activity (134.5%), while glibenclamide showed 62.5 and 133.0% activity, respectively. None of the extracts affected blood insulin. Presence of sugar in dried aqueous extract could suppress the hypoglycemic effect during the first hour of the experiment. After 1 h, the hepatic mechanism overwhelmed and thus lowering effect in blood glucose appeared. The conclusion is that C. bruguierana ssp. belangerana is able to lower blood glucose via stimulation of hepatic glycogenolysis and inhibition of gluconeogenesis.
  Mahban Rahimifard , Mona Navaii-Nigjeh , Amir Nilli-Ahmadabadi , Nazila Pourkhalili , Maryam Baeeri , Azadeh Mohammadirad and Mohammad Abdollahi
  Glycyrrhizic Acid (GA) a major component of licorice, has been reported to have potent antioxidant effects and used widely throughout the world. In the present study, the effects of GA on the function, viability and level of Reactive Oxygen Species (ROS) in isolated rat pancreatic islets were evaluated. After Laparotomy, pancreas was removed and islets were isolated and incubated in RPMI 1640 for 24 h and then islets were separated. GA at logarithmic doses (1, 10, 100 and 1000 μM) were added to islets and incubated for 24 h and then static insulin secretion was tested. Also, viability of cells and their ROS level were determined using Mitochondrial Toxicity Test (MTT) and fluorometric assay. Then islets were stained by dithizone and observed under microscope. The results of MTT test indicated that rang of 1-100 μM of GA is safe. In the dose of 1000 μM, GA increased ROS and reduced viability of islets. GA at 1 μM significantly increased secretion of insulin via isolated islets in the presence of stimulation level of glucose (16.7 mM). Results of dithizone staining showed a reduction in live cells at high dose of GA. The LC50 study was done to determine the toxicity of GA on rat pancreatic islets and a 24 h LC50 of 15 mM was found. GA showed remarkable anti oxidative effects at low doses and improved islet’s viability and insulin secretion in stimulation level of glucose. Interestingly, high dose of GA induced oxidative stress and reduced function of islets. The results of the present study indicate that GA is a good candidate to be examined in islet transplantation procedures to maintain islets viable and functional.
  Amir Baghaei , Neda Hajimohammadi , Maryam Baeeri , Azadeh Mohammadirad , Shokoufeh Hassani and Mohammad Abdollahi
  Aluminum phosphide (AlP) intoxication is becoming a major concern worldwide due to its high mortality rate (30-100%) besides non-availability of effective antidote till date. The aim of present study was to determine the effect of IMOD, a novel mixed herbal medicine on energy depletion, oxidative stress and change of electrocardiographic (ECG) parameters in the heart tissue of the rats poisoned by AlP. IMOD at doses of 13, 20 and 30 mg kg-1 was administered intraperitoneally 30 min after gavage intragastric administration of AlP (0.25 LD50). Sodium bicarbonate was used as the control. After anesthesia, animals were rapidly connected to PowerLab® device for monitoring of ECG, blood pressure and heart rate for 180 min. At 24 h post treatment, rats were decapitated and hearts removed for evaluation of oxidative stress markers and production of energy. AlP ingestion led to significant heart rate and blood pressure decrement as well as ST variation and shortening of PR interval. Administration of IMOD normalized AlP-disturbed cardiovascular parameters. IMOD also restored heart energy via re-establishment of cellular ATP pool and elimination of oxidative stress markers. These findings confirm the potential benefits of IMOD as an effective treatment for acute AlP poisoning that remain to be trialed clinically.
  Background and the purpose of the study: Date palms (Phoenix dactylifera L., Arecaceae) are one of the oldest cultivated plants which are used in folk medicine for treatment of various diseases. Due to the presence of antioxidant compounds in this plant and the role of oxidative stress in pathogenesis of chronic diseases, the aim of this study was to determine the antioxidant activity and total phenolic contents of date palm fruits extracts with water, methanol 50%, DMSO, and mixture of water-methanol-acetone-formic acid (20:40:40:0.1). Methods: Antioxidant activity of extracts were measured by two tests: inhibition of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and FRAP (Ferric Reducing Antioxidant Power).
Results and major conclusion: Among 10 different varieties which were examined, the DMSO extract of Khenizi showed the highest antioxidant activity with the FRAP value of 3279.48 µmol/100 g of the dry plant and DPPH inhibitory percentage of 56.61%. DPPH scavenging radical and FRAP values of some varieties including Khenrizi, Sayer, Shahabi and Maktub showed a significant increase and were comparable to α-tocopherol (10 mg/L) when extracted by DMSO. Formic acid extract of Shahabi variety with 276.85 mg GAE/100 g of the dry plant showed the highest total phenolic content compared to other varieties. There was no correlation between accumulation of total phenol and antioxidant activity of extracts, explaining existence of other antioxidant components in date.
  Sanaz Shahriari , Narguess Yasa , Azadeh Mohammadirad , Reza Khorasani and Mohammad Abdollahi
  Rosa damascena Mill, (Rosaceae) is a widely cultivated ornamental plant. Several therapeutic effects including calmative, antianxiety, laxative and antispasmodic have been described for the flower of R. damascena. The petals of R. damascena are specially used as cardiotonic by the people of Guilan province. In this study antioxidant potential of R. damascena petals were determined by FRAP test and its ability to inhibit lipid peroxidation was determined by TBARS test in rat. In vivo examination was performed by oral administration of ethanol extract of R. damascena petals at doses of 50, 75, 100 and 200 mg/kg/day for 10 days which compared to vitamin E (10 mg/kg/day) and control groups. In vivo evaluation of antioxidant effects of R. damascene with these two methods showed that the extract of R. damascena has a high ability to inhibit lipid peroxidation and has a high antioxidant power with all doses comparing to control (p<0.001). The highest activity was observed with the dose of 200 mg/kg/day. This preliminary study indicates the interesting anti oxidative stress activity of R. damascena, which is comparable to the known antioxidant compound, alpha-tocopherol. R. damascena can be considered as a medicinal source for the treatment and prevention of many free radicals related diseases.
  Azadeh Mohammadirad and Mohammad Abdollahi
  The purpose of this study was to provide a systematic review on the animal or human evidences linking aluminium (Al) toxicity to oxidant/antioxidant imbalance. Embase, Scopus, Pubmed, Web of Science, Google Scholar and SID databases were searched up to 1st October 2010. Over 50 studies including animal and human linking oxidative stress to Al were reviewed. Most of animal and human studies show a significant increase in lipid peroxidation (LPO) by Al. The maximum LPO was reported in the brain. Data about changes of enzymatic antioxidants such as Superoxide Dismutase (SOD), Catalase (CAT), Glutathione Peroxidase (GPx) post exposure to Al are controversial. Animal studies showed that vitamin E, C, melatonin and pinoline reduce LPO in Al-exposed subjects. Al can affect body oxidant/antioxidant balance in favor of oxidative toxic stress. Among parameters tested in various studies, LPO seems the best indicator of Al toxicity. The role of iron homeostasis in mediation of cytotoxic effects of Al seems important. Since, oxidant/antioxidant imbalance is involved in the pathogenesis of many diseases including inflammatory bowel diseases, diabetes, osteoporosis, it would not be surprising to track roles of Al in many deliberating diseases in future.
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