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Articles by Ayman A. El-Badry
Total Records ( 3 ) for Ayman A. El-Badry
  Khalil H. Al-Ali , Hesham A. El-Beshbishy , Abdulaziz A. Alghaithy , Hossam Abdallah , Ayman A. El-Badry and Essam Abdel-Sattar
  Labiatae family is represented in Saudi Arabia. It is one of the most distinctive families of flowering plants with a great diversity. This study aimed to investigate in vitro antioxidant and antiprotozoal activities of methanolic extract of Mentha longifolia (ML) and Origanum syriacum (OS). OS exhibited high phenolic contents (mg gallic acid equivalent g-1 weight) 87±3.5 mg GAE g-1. ML produced 23±3.2 mg GAE g-1. Using potassium ferricyanide method, OS exhibited high absorbance after 120 h (0.53±0.037) that was directly proportional to the reducing power, however, ML produced lower absorbance (0.38±0.031). Percentage scavenging activity against diphenyl-2-picryl-hydrazyl radical was 875 (OS) and 128% (ML). Absorbencies of ferric thiocyanate assay was 0.86 and 0.25 for ML and OS, respectively. Using phosphomolybdenum method, OS exhibited antioxidant activity (80%) followed by ML (55%). Percentage superoxide scavenging activities was 47 (OS) and 15% (ML). OS exhibited antimalarial activity against P. falciparum-K1 strain in contrast to ML. OS extract exhibited activity against T. cruzi. ML exhibited activity against T. cruzi. OS exhibited activity against T. b. brucei. However, ML showed no marked activity against T. b. brucei. ML and OS exhibited no marked activity against L. infantum. Both ML and OS exhibited no marked cytotoxic activity against MRC-5 cells. It was concluded that, ML and OS have the potency to act as powerful antioxidants and to some extent as antiprotozoal.
  Khalil H. Al-Ali , Hesham A. El-Beshbishy , Ayman A. El-Badry and Moussa Alkhalaf
  Labiatae family is represented in Saudi Arabia. The aim of the present study was to go insight to investigate the anticancer activity and antioxidative potentials of methanolic extracts of Mentha longifolia L. (ML) and Ocimum basilicum L. (OB) that grown in Madina province, western region, Saudi Arabia. OB exhibited the greater phenolic contents as mg gallic acid equivalent/g weight (mg GAE/g) for a value of 105±5.5 mg GAE/g. On the other hand, ML produced 29±3.12 mg GAE/g. The standard antioxidant vitamin E used in this experiment elicited a value of total phenolic contents equal 22±2.2 mg GAE/g. The percentage scavenging activity of against diphenylpicrylhydrazyl (DPPH.) was 850 and 160% for OB and ML extracts, respectively. Vitamin E elicited% scavenging activity of against DPPH. equal to 198%. Brine shrimp cytotoxic assay clearly indicated the cytotoxic effects of either ML or OB extract. The brine shrimp survival is inversely proportional to the concentration of either ML or OB extract used with LD50 191.23 and 235.50 ppm, respectively. Toxic effects on brine shrimps indicated the anticancer potential of ML or OB extract. The ML or OB extract was unable to produce pbluescript (pBS) plasmid DNA damage, while the plasmid DNA treated with EcoRI produced a single band as a result of DNA damage. Also, both ML and OB extract exhibited marked cytotoxic activity against MCF-7 cells at various concentrations (20, 40, 80, 160 and 320 μg mL-1). The 160 and 320 μg mL-1 showed more cytotoxic effect against MCF-7 cells. Based on results achieved, we can concluded that, OB and ML extracts have the potency to act as powerful antioxidants and protect against DNA damage and have cytotoxic activity against MCF-7 cell line.
  Ayman A. El-Badry , Abdullah M. Al-Juhani , El-Kheir D. Ibrahim and Saleem F. Al-Zubiany
  An entomological survey was carried out in four provinces representing the surroundings of Al-Madinah Al-Munawarah, Western Saudi Arabia from May 2007 to April 2008, to determine diversity, seasonal variations and bionomics of sand flies, for better planning of their control strategies and prevention of leishmaniasis. Sand flies were collected using CDC light traps and sticky traps. A total of 4411 sand flies were collected. Six species belonging to two genera were identified; P. papatasi, P. sergenti, P. bergeroti, S. antennata, S. sergenti and S. clydei. P. papatasi was the predominant anthropophagic species (>80%). Sand flies were abundant intra-domiciliary (>75%) (mainly Phlebotomus) than extra-domiciliary with highly significance (mainly Sergentomyia), intra-domiciliary abundance was inflated by a greater proportion of female flies. Of the 845 dissected Phlebotomus females, Leishmania infection rate was ranged from 0.3 to 1.5%, mainly June and sometimes May and October which give spotlight on infectivity of sand flies and planning of control strategies, 362 (42.8%) were gravid, 417 (49.3%) were blood fed; Regarding parity and fecundity, the striking finding was that none of the dissected females were parous in January, it shed light on the mating season habits of sand flies in Almadinah region and to design novel control strategies.
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