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Articles by Ayhan Filazi
Total Records ( 2 ) for Ayhan Filazi
  Ulvi Reha Fidanci , Hidayet Yavuz , Cavit Kum , Funda Kiral , Mehmet Ozdemir , Selim Sekkin and Ayhan Filazi
  It was aimed to evaluate the effects of accumulated ammonia (NH3) concentration in poultry housing and Nitrite (NO2-) Nitrate (NO3-) concentrations in poultry litter on thyroid hormone levels (Triiodothyronine (T3) and Thyroxine (T4)), Body Weight (BW) and variables parameters such as blood methemoglobin, serum retinol, β-carotene and total cholesterol levels. Weighing 58.0±3.2 g (control group) and 60.0±4.3 g (experiment group), 1 day old, 180 male broiler chickens were used. Chicks were allowed ad libitum access to feed and water throughout the 45 days trials. In the experiment group, the ventilation was restricted without changing other conditions. NH3 concentration in poultry housing and moisture ratio, as well as NO2--NO3- concentrations in litter were measured with 5 days intervals throughout the 45 days trials. Plasma total T3, T4, blood methemoglobin and serum retinol, β-carotene and total cholesterol levels were evaluated at the 45 days. NH3 concentration in poultry housing was increased after 21 days (p<0.05) at 0.222 g bird-1 day and 26 days (p<0.01) at range 0.377-0.400 g bird-1 day in the experiment group as compared with the control group throughout the 45 days trials. In addition, moisture ratio in litter were increased after 26 days (p<0.05) at 0.444% bird-1 day and 36 days (p<0.01) at range 0.461-0.472% bird-1 day. Also, NO2- concentrations in litter were increased after 26 days (p<0.05) at range 7.50-8-40 ppm bird-1 day. As to NO3- concentrations in litter in the experiment group, no statistically significant difference was observed. Compared to control group, at 45 days, BW, plasma total T3, as well as serum retinol and β-carotene levels decreased significantly in experiment group (p<0.01). Total cholesterol level was increased (p<0.05). No statistically considerable differences were found in plasma total T4 and blood methemoglobin levels.
  Filiz Sen and Ayhan Filazi
  Researchers describe the development of a Novel Method that is sensitive and reliable for detecting isoxazolyl penicillins (Cloxacillin (CLO) and Dicloxacillin (DCL)) in commercially available cow milk using High-Performance Liquid Chromatography with UV-Diode Array Detection (HPLC-DAD). CLO and DCL were extracted via deproteinization of milk samples, adjusting the pH to 4.5-5 using hydrochloric acid and then adding acetone/chloroform, followed by centrifugation and cleanup using a 0.45 μm nylon filter. CLO and DCL were separated using an Inertsil ODS-C18 column (4.6x250 mmx3.5 μm) via elution with a mobile phase consisting of 0.05 M phosphate buffer solution at pH 4.7 and acetonitrile at 22°C. Recovery of CLO and DCL was 96.47-106.94 and 97.76-104.86%, respectively. The limit of quantifications were 23.15 μg kg-1 for CLO and 2.28 μg kg-1 for DCL which are lower than the limits for residues in cow milk established by the European Union, indicating that the method is suitable for performing routine analysis. In order to determine this Novel Method’s utility in a laboratory environment for analyzing real raw milk samples 100 raw milk samples were obtained from raw milk tanks belonging to milk producers. CLO and DCL were not detected in any of the samples. The present findings indicate that the Novel Detection Method is simple, rapid, sensitive and reliable, as compared to other detection methods and that as it uses fewer chemicals and does not require derivatization it could be considered the preferred method.
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