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Articles by Ayhan ATASEVER
Total Records ( 3 ) for Ayhan ATASEVER
  Meryem Eren , Fatma Uyanik , Berrin Kocaoglu Guclu and Ayhan Atasever
  This study was performed to investigate the effect of adding boron (B) to broilers’ diets on performance, some blood biochemical parameters and visual and histopathological examinations of organs in broilers. One hundred and forty four chicks were randomly assigned to 4 groups with 3 replicates. The broilers were fed commercial diets supplemented with 0 (control group) and 500, 750, 1000 mg kg-1 (diet) B from Boric Acid (BA) for 42 days. Live body weight, weight gain, food consumption and feed conversion ratio were recorded at weekly intervals. At the end of the experimental period (42 days of age), serum Aspartate aminotransferase (AST), Alanine aminotransferase (ALT), Gamma Glutamyl Transpeptidase (GGT), Alkaline Phosphatase (ALP), Creatine Kinase (CK), Lactate Dehydrogenase (LDH) enzyme activities and glucose, creatinine, total protein, albumin, globulin, triglyceride, total cholesterol, High Density Lipoprotein-cholesterol (HDL-C), Low Density Lipoprotein-cholesterol (LDL-C), calcium (Ca), phosphorus (P), magnesium (Mg) and B levels were determined. Performance parameters were affected negatively and significantly by B supplementation. Serum AST, ALT, CK, LDH activities and glucose, total protein, albumin, globulin, triglycerides, total cholesterol, LDL-C and Mg levels were significantly influenced by dietary B supplementation. Necrosis in muscle fibers was observed but no gross and microscopic changes were determined in liver, kidney and brain in histological examinations in B supplemented groups. In conclusion; add boron to broilers’ diets at levels 500, 750 and 1000 mg kg-1 (feed) did not cause mortality or result in histopathological lesions except for muscles. On the other hand, it resulted in a significant decrease in performance and changed some biochemical parameters in broilers.
  Latife BEYAZ , Ayhan ATASEVER , Feyzullah BEYAZ and Anıl ICA
  The expression of the calcium-binding protein S100 and its subunits α and β in the intracellular protozoon Eimeria tenella was investigated. For this purpose, anti-S100 whole molecule polyclonal antibody and anti-S100 α and anti-S100 β monoclonal antibodies were used on paraffin sections collected from the cecum, ileum, colon, rectum, and bursa of Fabricius of chicks experimentally infected with E. tenella. One hundred seventy-five 2- to 3-day-old specific pathogen-free chicks were used. The chicks were divided into experiment and control groups, to include 94 and 81 chicks, respectively. All the chicks in the experimental group received 10,000 spored oocytes in 0.5 ml inoculum in a single dose. The duration of the experiment was limited to 18 days. All 175 chicks were sacrificed by cervical dislocation between hours 1 and 432 following inoculation. The streptavidin-biotin complex method was used to detect the immunoreactivity of the antibodies used. The results revealed a positive immune reaction for the S100 whole molecule and for α and β subunits in the schizonts, microgametes, macrogametes, and oocytes of the E. tenella in the cecum, ileum, colon, rectum, and bursa of Fabricius. These results show that both α and β subunits of the S100 protein existed in all the endogenous phases of E. tenella. These proteins are likely to mediate the parasitic development of E. tenella within host cells.
  Latife Beyaz , Ayhan Atasever , Fuat Aydin , K. Semih Gumussoy and Secil Abay
  This study aimed to experimentally induce Salmonella enterica subsp. enterica serovar Enteritidis (Salmonella Gallinarum) infection in turkey poults in order to detect S. Gallinarum using immunohistochemical, bacteriological, and histological procedures. The study included 90 white turkey poults that were divided into 3 groups (1-day-old, 3-week-old, and 2-month-old), each of which was orally inoculated with an inoculum of 0.3 mL of S. Gallinarum strain 9 broth culture that contained approximately 2 x 104, 1 x 106, and 1 x 109 cfu/mL, respectively. The duration of the experiment was 18 days. Clinical, pathological, and bacteriological findings were evaluated daily until 18 days post inoculation (pi). The most evident clinical symptoms in all 3 groups were diarrhea and somnolence, especially in 1-day-old group. S. Gallinarum strain 9 was isolated from organs and cloacal swabs up to 16 days pi. Inflammation and typical granulomatous nodules in the internal organs were observed in all 3 groups. Strong immunoreactivity was determined in the lungs, bursa of Fabricius, caecum, ileum, and cloaca of all infected poults up to 18 days pi. In conclusion, S. Gallinarum infection caused heterophilic granulomas, especially in the duodenum of turkey poults, and immunohistochemical analysis can be considered as an adjunct to bacteriological methods in the diagnosis of S. Gallinarum infection.
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