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Articles by Ayesha Khan
Total Records ( 2 ) for Ayesha Khan
  Ayesha Khan , Ikram-ul-Haq , Waseem Ahmad Butt and Sikander Ali
  Present investigation deals with the isolation and screening of Aspergillus niger isolates for the biosynthesis of xylanase. About 104 different strains of Aspergillus niger were isolated from soil collected from different areas of Lahore by serial dilution method on xylene agar malt extract medium. The strains with larger whitish zones of xylene hydrolysis were picked up and transferred to the PDA slants. The cultures were incubated at 30°C for 3-5 days for maximal sporulation. The Aspergillus niger strains were screened for xylanase production by submerged fermentation. Xylanase activity was ranged from 30-225 U ml-1 and dry cell mass from 6.48-26.56 g L-1. Maximum xylanase productivity was obtained by GCBT-35 (225 U ml-1). The mycelial dry weight was 9.54 g L-1 while mycelia were small in size and rounded in shape.
  Ayesha Khan , Sikander Ali and Ikram-ul-Haq
  The present study is concerned with the optimization of cultural conditions for maximum conversion of L-Tyrosine to L-DOPA by strain of Aspergillus oryzae ISB-9. The effect of time course, temperature and pH on the production of L-DOPA was checked. Surface culture method was employed for L-DOPA fermentation. Optimum temperature and time for L-DOPA production is 50 C and 60 minutes respectively. However, the optimum pH of mycelium development was 5.0 because maximum production of L-DOPA (0.95 mg ml-1) was observed at this pH. The acidic pH (3.0) of reaction mixture was found to be optimum for higher L-DOPA production (1.1mg ml-1).
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