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Articles by Aya Saito
Total Records ( 2 ) for Aya Saito
  Ken Onda , Yukiko Emura , Aya Saito , Tooru Ikeyama , Reiichiro Sato , Hideharu Ochiai , Kazuyuki Kaneko , Tsunenori Iriki and Yasunori Wada
  Acute stress following surgical castration of calves is a common animal welfare concern. Administration of Non-Steroidal Anti-Inflammatory Drugs (NSAIDs) can reduce post-castration stress but treatments vary in cost and efficacy. In this study, the effectiveness of sodium salicylate in relieving stress during invasive castration of calves was assessed on the basis of changes in serum cortisol levels and was compared with the effectiveness of ketoprofen. Twenty male Holstein calves were divided into 4 groups: a sham-operated untreated group, a castrated untreated group and 2 castrated groups receiving NSAIDs (sodium salicylate or ketoprofen). NSAIDs were injected intravenously immediately before administering local anesthetic. Blood samples were collected at regular time intervals for measurement of cortisol concentrations. Serum cortisol concentrations of the calves that were administered sodium salicylate were lower than those of the calves that were not administered any NSAID at 1, 1.5 and 2 h after castration and higher than those of the calves that were administered ketoprofen at 2 h after castration (p<0.05). Although, the analgesic and anti-inflammatory effects of sodium salicylate are weaker than those of ketoprofen, the use of salicylic acid derivatives in surgical castration is useful from the viewpoint of animal welfare and entails a significantly lower cost.
  Akikazu Fujima , Yoshitsugu Ochiai , Aya Saito , Yuki Omori , Atsuya Noda , Yukumasa Kazuyama , Hiroshi Shoji , Kiyoshi Tanabayashi , Fukiko Ueda , Yasuhiro Yoshikawa and Ryo Hondo
  The antigenic cross-reactive characteristics of herpes B virus and herpes simplex virus (HSV) type 1 (HSV-1) and HSV-2 are responsible for false-positive diagnoses by serological assays in humans and macaques. In the present study, we developed a fluorometric indirect enzyme-linked immunosorbent assay (ELISA) with recombinant herpes B virus glycoprotein D (gD) and HSV-1 and HSV-2 gG (gG-1 and gG-2, respectively) to discriminate between the three primate herpesvirus infections. The secreted form of gD, gDdTM, was used to detect antibody to herpes B virus gD. Sera positive for herpes B virus, HSV-1, and HSV-2 showed specific reactions to gD, gG-1, and gG-2, respectively. Sera collected from humans and rhesus macaques were investigated for the presence of antibodies to the recombinant proteins of the three herpesviruses. The results suggested that the approach is able to discriminate between herpes B virus and HSV infections. The ELISA was also found to be able to detect infections with multiple primate herpesviruses and may have the potential to identify a subsequent infection in individuals that have already been infected with another herpesvirus. In addition, we found evidence of a greater cross-reactivity of herpes B virus with HSV-1 than with HSV-2. It is suggested that the ELISA with the recombinant antigens is useful not only for the serodiagnosis of primate herpesvirus infections but also for elucidation of the seroprevalence of herpesviruses in humans and primates.
 
 
 
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