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Articles by Awad G. Osman
Total Records ( 3 ) for Awad G. Osman
  Afrah T. Mohamed , Adil A. El Hussein , Marmar A. El Siddig and Awad G. Osman
  Three laboratory experiments were carried out to study the biodegradation of Oxyfluorfen herbicide (Goal) in a soil with no history of pesticides application at two temperatures (28 and 40°C) with or without the addition of mineral fertilizers (NPK). Different concentrations of Oxyfluorfen were applied to the soil samples and the herbicide residue was determined using Gas Liquid Chromatography (GLC) at 0 time and then at 15 days intervals for 45 days. Various genera of microorganisms were isolated on different semi-selective media from soils treated with different concentrations of Oxyfluorfen. The ability of these isolates to utilize Oxyfluorfen as a sole source of carbon and energy was studied. Results indicated that biodegradation of Oxyfluorfen in soil incubated at 40°C after 45 days of incubation was better (55.2-78.3%) than in soil incubated at 28°C (17.5-36.6%). Addition of mineral fertilizers (NPK) increased the biodegradation of Oxyfluorfen in soils. Intensive degradation (27.8-55.5%) was observed in NPK fertilized soils incubated at 40°C after 15 days of incubation at all Oxyfluorfen concentrations. Ten potential Oxyfluorfen degraders were identified. Results obtained showed that within 21 days, Bacillus spp. had the ability to degrade 80-95.6% of Oxyfluorfen followed by Pseudomonas sp. (82.2%), Arthrobacter spp. (82.2%), Aspergillus sp. (77.8%), Mycobacterium sp. (75.6%), Micrococcus sp. (73.3%) and Streptomyces sp. (68.9%). It could be concluded that biodegradation of Oxyfluorfen in soils is significantly affected by temperature and the microbial species. These microorganisms are considered as potential candidates for use in any program aiming at decontamination of Oxyfluorfen polluted sites.
  Gadalla A. Elhassan , Migdam E. Abdelgani , Awad G. Osman , Somaya S. Mohamed and Belgees S. Abdelgadir
  not available
  Adil A. El Hussein , Afrah T. Mohamed , Marmar A. El Siddig , Ashraf M. Sherif and Awad G. Osman
  This study was conducted under laboratory conditions to investigate the effect of different concentrations of oxyfluorfen herbicide on the numbers and diversity of the main groups of soil microflora in two soil types. The numbers of microorganisms in oxyfluorfen treated soils were determined at zero time and then at 15 days interval for 45 days. Oxyfluorfen residue in soils was determined by Gas Liquid Chromatography (GLC). Results showed that the numbers of organic nitrogen users were inhibited at 15 days with maximum inhibition percentage (65.2%) observed in Elsilait soil treated with 4000 mg kg-1. The highest increment percentage (97.8%) was observed in Gerif soil sample treated with 400 mg kg-1 after 30 days. Maximum inhibition percentage in inorganic nitrogen users was 42.4% which was recorded for soil treated with 96 mg kg-1 herbicide. Numbers of Mycobacterium spp. which were absolutely dominant in the nitrate agar medium decreased as from the beginning of the incubation period up to 45 days. The highest increment percent (180.1%) in fungal population was observed in Elsilait soil treated with 4000 mg kg-1. At low concentrations of the herbicide, degradation started after 15 days of incubation while at higher levels, a noticeable degradation was observed after 30 days of incubation in Elsilait loam soil. In Gerif silt loam soil the degradation percentages of the herbicide were generally very low at all concentrations tested compared to those observed in Elsilait loam soil. It could be concluded that the effect of oxyfluorfen on soil microorganisms depends on the group of microorganisms and soil type. While the growth of fungi, organic and inorganic nitrogen users, is enhanced, at least in low oxyfluorfen concentrations, the growth of Mycobacterium was inhibited at all concentrations tested. Higher degradation percentages were recorded in Elsilait soil (55.2-78.3%) compared to Gerif soil (6.0-9.5%).
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